LOCUS       sequence-329878-        7035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal
            mRFP fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, 
            Jin WL, Frost A, Polleux F
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required for
            neuronal migration and morphogenesis.
  JOURNAL   Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047.
  PUBMED    19737524
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.06"; journalName: "Cell"; date: "2009-09-4- 4";
            volume: "138"; issue: "5"; pages: "990-1004"
COMMENT     Sequence Label: pCAG-SRGAP2A-FBAR-mRFP
FEATURES             Location/Qualifiers
     source          1..7035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        34..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        415..692
                     /label=chicken beta-actin promoter
     intron          693..1709
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1633..1655
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1717..1736
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3288..3965
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3288..3965
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3418..3436)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3686..3705
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3880..3903
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4056..4075)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4121..4176
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4175..4194)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4567..4583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4567..4583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4580..4602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4591..4607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4615..4621,4622..4639,4640..4645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4660..4681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4740..4935
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4786..4921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4941..5075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4978..4997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5032..5051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5143..5160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5314..5902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5394..5413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6073..6864,6865..6933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6696..6715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6934..7035,1..3))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      421 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      481 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
      541 cgccrggsgg ggsggggsgg ggsgrggggs ggggsggggs grggcggaga ggtgcggcgg
      601 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      661 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
      721 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
      781 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
      841 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
      901 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
      961 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1021 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     1081 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     1141 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     1381 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     1441 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     1501 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1561 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     1621 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1681 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     1741 ctcatcattt tggcaaagaa ttgctcgagg ccaccatgac gtctccagcc aaattcaaaa
     1801 aggataagga gatcatcgca gagtacgata ctcaggtcaa agagattagg gctcagctca
     1861 cagagcagat gaaatgccta gatcagcaat gtgagctccg ggtgcagctg ttgcaggacc
     1921 tgcaggactt cttccgcaag aaggctgaga ttgaaatgga ctactctcga aacctggaga
     1981 agctagccga gcgctttcta gccaagacac gaagcaccaa ggaccagcaa tttaagaagg
     2041 accagaatgt tctttctcca gtcaactgct ggaacctcct cttaaaccaa gtgaagcggg
     2101 agagcaggga tcataccacc ctgagtgaca tctacctgaa taatataatt cctcgatttg
     2161 ttcaagtcag cgaggactca ggaagactct ttaaaaagag taaagaagtt ggccagcaac
     2221 tccaagatga tttgatgaaa gtcctgaacg agctttactc ggtcatgaag acatatcaca
     2281 tgtacaatgc cgacagcatc agtgctcaga gcaaactgaa ggaagcagag aaacaagaag
     2341 agaagcaaat tggcaaatca gtaaagcaag aggaccggca gacccctcgc tcccctgact
     2401 ccacagccaa tgtccgcatt gaggagaagc atgtccggag gagctcagtg aagaagattg
     2461 agaagatgaa ggagaagcga caagccaagt acacagaaaa taagctgaag gccattaaag
     2521 cccggaatga gtatttattg gctttggagg caaccaatgc atctgtcttc aagtactata
     2581 tccatgacct gtctgatatt attgatcaat gttgtgacct aggctaccat gctagcctga
     2641 accgggctct acgcactttc ctatctgctg aattaaatct ggaacagtca aaacatgaag
     2701 gtctggatgc tattgaaaat gcagtagaaa acctagatgc caccagtgac aagcaacggc
     2761 tcatggagat gtacaacaat gttttttgcc cccctatgaa atttgaattc cagccccaca
     2821 tgggagatat ggcctctcaa ctctgtgccc agcagcctgt ccagagtgag ctggtgcaga
     2881 gatgccaaca gctgcagtct cgcttatcca ctttgaagat tgagaatgaa gaggtgaaaa
     2941 agacaatgga ggccaccctg cagaccattc aagacattgt gactgttgag gattttgatg
     3001 tatctgactg cttccagtat agcaattcca tggagtctgt caaatcaact gtctctgaaa
     3061 cattcatgag caagcccagc attgctaaga ggagagcgaa ccagcaagag acggaacagt
     3121 tttatttcac gaaaatgaag gagtacttgg agggtaggaa cctcatcaca aagctacaag
     3181 ccaagcatga cctcctgcag aaaaccctgg gagaaagtca gcggacagac tgtagtcttg
     3241 ccaggcgaag ctcaactgtg aggaaacagg attccagcgc ccgggcaatg gcctcctccg
     3301 aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
     3361 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3421 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     3481 tccagtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     3541 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     3601 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     3661 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     3721 cctccaccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagatgaggc
     3781 tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga
     3841 agcccgtgca gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca
     3901 acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg
     3961 cctaagcggc cgcaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     4021 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     4081 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     4141 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     4201 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     4261 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     4321 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4381 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4441 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     4501 tccctcgacc tgcaccgtcg accagctkgt cgacrgtgca ccgtcgacca gcttggcgta
     4561 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4621 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4681 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     4741 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4921 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     4981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5041 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     5101 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5161 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5221 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5281 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5341 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5521 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5641 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5701 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5761 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5821 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5941 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6001 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6061 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6121 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6181 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6241 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6301 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6361 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6421 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6481 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6541 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6601 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6661 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6721 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6781 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6841 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6901 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6961 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7021 acaaataggg gttcc
//
