LOCUS       sequence-329893-        8874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Ollas.C.V5_NGFR
FEATURES             Location/Qualifiers
     source          1..8874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        317..392
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    440..557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        697..1875
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          928..1866
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1823..1843
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2026..2067
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2089..2124
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             2143..2184
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2947..3535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3000..3020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3418..3429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3610..3843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3915..4036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3952..3971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4006..4025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4076..4211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4231..4250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4232..4250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4260..4277)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4260..4276)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4269..4291)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4418..4873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4505..4524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4715..4736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4899..5003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5004..5072,5073..5864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5222..5241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6035..6623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6524..6543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6777..6794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6911..6932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6947..6952,6953..6970,6971..6977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6985..7001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6990..7012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7009..7025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7009..7025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7044..7064
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7046..7064
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7092..7318
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7319..7499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7546..7671
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8164..8397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8582..8626
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cgatcacgag actagtatcg agcggccgcc cccttcaccg agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 gtcttcgaga agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 atcaacttga aaaagtggca ccgagtcggt gcttttttta attctcgacc tcgagacaaa
      421 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      481 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      541 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
      601 tcgaggcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      661 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      721 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      781 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      841 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      901 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      961 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     1021 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     1081 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     1141 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     1201 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     1261 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     1321 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1381 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1441 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1501 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1561 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1621 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1681 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1741 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1801 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1861 tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc gggatccacg
     1921 gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg cctgctgctg
     1981 ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcagcgg cttcgccaac
     2041 gagctgggcc ccaggctgat gggcaagtgc ggaggaggcg ggggagggga ggaccaggtg
     2101 gaccccaggc tgatcgacgg caagggagga ggcgggggag ggggcaaacc aattccaaac
     2161 ccactgctgg gactggattc cacacccaca ggcctgtaca cacacagcgg tgagtgctgc
     2221 aaagcctgca acctgggcga gggtgtggcc cagccttgtg gagccaacca gaccgtgtgt
     2281 gagccctgcc tggacagcgt gacgttctcc gacgtggtga gcgcgaccga gccgtgcaag
     2341 ccgtgcaccg agtgcgtggg gctccagagc atgtcggcgc cgtgcgtgga ggccgacgac
     2401 gccgtgtgcc gctgcgccta cggctactac caggatgaga cgactgggcg ctgcgaggcg
     2461 tgccgcgtgt gcgaggcggg ctcgggcctc gtgttctcct gccaggacaa gcagaacacc
     2521 gtgtgcgagg agtgccccga cggcacgtat tccgacgagg ccaaccacgt ggacccgtgc
     2581 ctgccctgca ccgtgtgcga ggacaccgag cgccagctcc gcgagtgcac acgctgggcc
     2641 gacgccgagt gcgaggagat ccctggccgt tggattacac ggtccacacc cccagagggc
     2701 tcggacagca cagcccccag cacccaggag cctgaggcac ctccagaaca agacctcata
     2761 gccagcacgg tggcaggtgt ggtgaccaca gtgatgggca gctcccagcc cgtggtgacc
     2821 cgaggcacca ccgacaacct catccctgtc tattgctcca tcctggctgc tgtggttgtg
     2881 ggccttgtgg cctacatagc cttcaagagg tggaacaggg gtatcctcta gagcggccgc
     2941 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3001 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3061 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3121 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3181 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3241 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3301 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     3361 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3421 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3481 catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg
     3541 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3601 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     3661 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3721 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3781 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3841 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3901 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3961 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4021 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4141 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4201 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4261 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4321 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4381 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4441 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4501 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4561 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4621 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4681 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4741 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4801 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4861 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5221 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5521 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6901 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6961 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7021 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7081 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7141 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg tggaagtaag
     7201 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7261 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7321 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7381 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7441 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7501 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7561 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7621 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7681 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7741 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7801 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7861 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7921 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7981 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8041 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8101 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8161 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8221 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8281 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8341 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8401 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8461 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8521 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8581 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8641 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8701 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8761 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     8821 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatc
//
