LOCUS       sequence-329935-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length SRGAP2 with a C-termonal EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Guerrier S, Coutinho-Budd J, Sassa T, Gresset A, Jordan NV, Chen K, 
            Jin WL, Frost A, Polleux F
  TITLE     The F-BAR domain of srGAP2 induces membrane protrusions required for
            neuronal migration and morphogenesis.
  JOURNAL   Cell. 2009 Sep 4;138(5):990-1004. doi: 10.1016/j.cell.2009.06.047.
  PUBMED    19737524
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.06"; journalName: "Cell"; date: "2009-09-4- 4";
            volume: "138"; issue: "5"; pages: "990-1004"
COMMENT     Sequence Label: pCAG-SRGAP2A-EGFP
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        59..438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        440..717
                     /label=chicken beta-actin promoter
     intron          718..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1658..1680
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1742..1761
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5060..5069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5066..5068,5069..5071,5072..5785)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5066..5068,5069..5071,5072..5782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5111..5132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5372..5391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5719..5740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5877..5896)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5942..5997
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5996..6015)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6388..6404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6388..6404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6401..6423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6412..6428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6436..6442,6443..6460,6461..6466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6481..6502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6561..6756
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6607..6742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6762..6896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6799..6818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6853..6872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6964..6981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7135..7723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7215..7234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7894..8685,8686..8754))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8517..8536
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8755..8831,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
       61 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      421 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      481 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      541 ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg ggsggggsgr ggggsggggs
      601 ggggsgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      661 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      721 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      781 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      841 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      901 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      961 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     1021 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1081 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1141 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1201 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1261 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1321 ccgcctcggg ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1381 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1441 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1501 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1561 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct cgagctcaag
     1801 ctaattcatg acgtctccag ccaaattcaa aaaggataag gagatcatag cagagtacga
     1861 tactcaggtc aaagagatcc gtgctcagct cacagagcag atgaaatgcc tggaccagca
     1921 gtgtgagctt cgggtgcaac tgttgcagga cctccaggac ttcttccgaa agaaggcaga
     1981 gattgagatg gactactccc gcaacctgga gaagctggca gaacgcttcc tggccaagac
     2041 acgcagcacc aaggaccagc aattcaagaa ggatcagaat gttctctctc cagtcaactg
     2101 ctggaatctc ctcttaaacc aggtgaagcg ggaaagcagg gaccatacca ccctgagtga
     2161 catctacctg aataatatca ttcctcgatt tgtacaagtc agcgaggact caggaagact
     2221 ctttaaaaag agtaaagaag tcggccagca gctccaagat gatttgatga aggtcctgaa
     2281 cgagctctac tcggtgatga agacatatca catgtacaat gccgacagca tcagtgctca
     2341 gagcaaacta aaggaggcgg agaagcagga ggagaagcaa attggtaaat cggtaaagca
     2401 ggaggaccgg cagaccccac gctcccctga ctccacggcc aacgttcgca ttgaggagaa
     2461 acatgtccgg aggagctcag tgaagaagat tgagaagatg aaggagaagc gtcaagccaa
     2521 gtacacggag aataagctga aggccatcaa agcccggaat gagtacttgc tggctttgga
     2581 ggcaaccaat gcatctgtct tcaagtacta catccatgac ctatctgacc ttattgatca
     2641 gtgttgtgac ttaggctacc atgcaagtct gaaccgggct ctacgcacct tcctctctgc
     2701 tgagttaaac ctggaacagt cgaagcatga gggtctggat gccatcgaga atgcagtaga
     2761 aaacctggat gccaccagtg acaagcagcg cctcatggag atgtacaaca acgtcttctg
     2821 cccccctatg aagtttgagt ttcagcccca catgggggat atggcttccc agctctgtgc
     2881 ccagcagcct gtccagagtg agctggtaca gagatgccaa caactgcagt ctcgcttatc
     2941 cactctaaag attgaaaacg aagaggtaaa gaagacaatg gaggccaccc tgcaaaccat
     3001 ccaggacatt gtgactgtcg aggactttga tgtgtctgac tgcttccagt acagcaactc
     3061 catggagtcc gtcaagtcca cggtctctga aaccttcatg agcaagccca gcattgctaa
     3121 gaggagagcc aaccagcaag agacagagca gttttatttc acaaaaatga aagagtacct
     3181 ggagggcagg aacctcatca ccaagttaca agccaagcat gaccttctgc agaaaaccct
     3241 gggagaaagt cagcggacag attgcagtct agccaggcgc agctcaactg tgaggaaaca
     3301 ggactccagc caggcaattc ctctggtggt ggaaagctgt atccggttta tcagcagaca
     3361 cggactacag catgaaggaa ttttccgggt gtcaggatcc caggtggaag tgaatgacat
     3421 caaaaatgcc tttgagagag gagaggaccc cctggctggg gaccagaacg accatgacat
     3481 ggattccata gctggtgtcc tgaagcttta cttccggggg ctggaacacc ctctcttccc
     3541 caaggacatc tttcatgacc tgatggcctg cgtcacaatg gacaacctgc aggagagagc
     3601 tctgcacatc cggaaagtcc tcctagtcct gcccaaaacc actctgatta tcatgagata
     3661 cctctttgcc ttcctcaatc atttatcaca gttcagtgaa gagaacatga tggaccccta
     3721 caacctcgcc atctgcttcg ggccctcgct aatgtcagtg ccagagggcc acgaccaggt
     3781 gtcctgccaa gcccacgtga atgagctgat caaaaccatc atcatccagc atgagaacat
     3841 cttcccaagc cccagggagc tggagggccc tgtctacagc agaggaggaa gcatggagga
     3901 ttactgtgat agccctcatg gagagactac ctcggttgaa gactcaaccc aggatgtgac
     3961 cgcagagcac cacacgagcg atgacgaatg tgagcccatc gaggccattg ccaagtttga
     4021 ctacgtgggc cggacagccc gagagctgtc ctttaagaag ggagcatccc tgctgcttta
     4081 ccagcgggct tccgacgact ggtgggaagg ccggcacaat ggcatcgacg gactcatccc
     4141 ccatcagtac atcgtggtcc aagacaccga ggacggtgtc gtggagaggt ccagccccaa
     4201 gtctgagatt gaggtcattt ctgagccacc tgaagaaaag gtgacagcca gagcgggggc
     4261 cagctgtccc agtgggggtc atgtagccga tatttatctt gcaaacatca acaagcaaag
     4321 gaagcgtcca gaatctggga gcatccggaa aacttttcgg agtgacagcc atgggctgag
     4381 cagttccctg actgactcct cctccccagg ggtgggggct agctgccgcc catcctccca
     4441 gcccatcatg agccagagcc tccccaaaga agggccagat aagtgttcca tcagtgggca
     4501 cgggagcctc aactccatca gccgccactc atccctgaag aatcggctgg atagtccaca
     4561 gatccggaag actgccacag cgggaaggtc aaaaagcttc aataaccatc ggcccatgga
     4621 ccctgaggtc attgctcagg atattgaggc aacaatgaac tcggccctga atgagctacg
     4681 ggaactagaa cggcagagca gtgtcaaaca cacccctgac gtggttctgg acaccttgga
     4741 gcccctcaaa acctccccag tggtggcccc cacgtcagag ccctccagcc ctctgcacac
     4801 ccagctcctc aaggaccccg agcccgcctt ccagcgcagc gccagtactg ctggggacat
     4861 cgcctgcgcc ttccggcctg tcaagtctgt caagatggct gccccggtca aaccaccagc
     4921 cacacggccc aagcccactg tcttccccaa aacaaatgcc actagccctg gtgtcaactc
     4981 atcaacttcc ccacagtcta ctgacaagtc ttgtactgtc gctcgatcga cggtaccgcg
     5041 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     5101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5221 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5281 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     5581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     5641 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     5701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     5761 cggcatggac gagctgtaca agtaaagcgg ccgcaattca ctcctcaggt gcaggctgcc
     5821 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5881 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5941 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     6001 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     6061 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6121 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6181 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6241 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6301 gtccctcttc tcttatggag atccctcgac ctgcaccgtc gaccagctgg tcgacggtgc
     6361 accgtcgacc agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6541 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6601 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6661 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6721 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     6781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     6901 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7141 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7561 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7801 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7861 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7921 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7981 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8041 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8101 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8161 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8221 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8281 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8341 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8401 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8461 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8521 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8581 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8641 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8701 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8761 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8821 tgaatgtatt t
//
