LOCUS       sequence-329936-        9545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of rat WT Myosin Va with deletion of the 1005 
            N-terminal residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.Halo-myosin Va tail 1006-1828
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1636..1658
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1750..1770
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1786..2670
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     polyA_signal    5381..5515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5418..5437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5472..5491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5546..5562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5546..5562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5559..5581)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5570..5586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5594..5600,5601..5618,5619..5624))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5639..5660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5777..5794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5948..6536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6028..6047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6707..7498,7499..7567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7330..7349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7568..7672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7740..7758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7796..7818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7918..7937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8131..8153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8145..8162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8146..8162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcactg cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgrgggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      721 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
     1021 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
     1081 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1141 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1201 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1261 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggrgggct
     1321 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1381 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1441 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1501 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1561 gtccccttct ccatctccag cctcggggct gtccgcaggg ggacggctgc cttcgggggg
     1621 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt
     1681 ctctgttcct ccgcagcccc caagcttggc gcgcctaggc ctaccatggc tagcctcgag
     1741 ccaaccactg aggatctgta ctttcagagc gataacgatg gatccgaaat cggtactggc
     1801 tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt
     1861 ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg
     1921 tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc
     1981 ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc
     2041 atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg
     2101 ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca
     2161 tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag
     2221 accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt
     2281 tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac
     2341 cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac
     2401 gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac
     2461 tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc
     2521 ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc
     2581 ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg
     2641 cgctggctgt ctactctgga gatttccggt ctgtacggaa tgcagaagct gatctcagag
     2701 gaggacctgc aaaaacttat ttctgaagag gatctgcaaa agcttatttc tgaagaagat
     2761 ctgtacaagg gaggtggctc tagcaaaaag tccattgaag aacgagcaga taaatacaaa
     2821 caagaaactg agcagctggt gtcaaacttg aaggaagaaa acactctgct gaagcaggaa
     2881 aaggagaccc tcaatcacct catggtggag caggctaagg agatgacaga aactatggag
     2941 aggaagttag tagaagaaac aaaacaactg gagctcgacc tgaatgatga gaggctgagg
     3001 tatcagaacc tcctgaatga gttcagtcgt ctggaggagc gctatgacga cctcaaggaa
     3061 gagatgaccc tgatgctgaa tgtgcctaag ccaggacaca agagaacaga ctctacccac
     3121 agcagcaatg agtctgaata caccttcagc tcagaatttg cagaaactga agacattgca
     3181 ccaaggacag aggagccaac tgagaagaag gtgcctctgg atatgtcatt gttccttaag
     3241 ctccagaagc gtgtcacaga gctggaacag gagaagcagt tgatgcagga tgagctggac
     3301 cgaaaggagg agcaggtgtt gcgcagcaag gcaaaggaag aagaaagacc acagattaga
     3361 ggtgctgaac tggaatatga gtccctcaag cgtcaggaac tggagtcaga aaacaaaaag
     3421 ctgaagaatg agctgaatga gctacgcaaa gccctgagtg agaagagtgc ccccgaagtg
     3481 actgcgccag gcgcgcccgc ttaccgcgtc ctcatggagc agctgacggc cgtgagcgag
     3541 gagctcgacg tccgcaagga ggaagtcctc atcttaaggt ctcagctggt gagccagaaa
     3601 gaagccatcc aacccaagga tgacaagaat acaatgacag attccacaat acttttggaa
     3661 gatgtacaga aaatgaaaga caaaggtgaa atagcacaag catatattgg tttgaaagaa
     3721 acgaacaggc tcttagaatc ccagctacag tcgcagaaga ggagccatga aaatgaggct
     3781 gaggccctcc gtggggagat ccagagccta aaggaagaaa acaaccggca acagcagctg
     3841 ctggcccaga acctgcagct gccccctgag gcccgcattg aggccagcct gcagcatgag
     3901 atcacccggc tgaccaatga aaacctggat ctgatggaac aacttgaaaa gcaggataaa
     3961 accgtccgga aactgaagaa acaactaaaa gtctttgcca aaaaaatcgg tgaactagaa
     4021 gtggggcaga tggagaacat atccccagga cagatcatcg atgagcctat ccggccggtc
     4081 aacattcccc ggaaagaaaa ggatttccaa gggatgctgg agtacaagcg ggaggacgaa
     4141 cagaagcttg ttaagaacct gattctagaa ctaaagccac gtggtgtggc tgtcaatctg
     4201 attccagggt taccggcata tatcttgttt atgtgtgtgc gacatgccga ctacctgaac
     4261 gatgatcaga aagtaagatc attgctgaca tcaacaatta acagcatcaa aaaagtcctg
     4321 aagaaaagag gtgacgattt tgaaactgtc tccttctggc tctctaacac atgtcgattt
     4381 ttgcactgtt tgaagcaata tagtggagaa gagggcttca tgaaacacaa cacgtctcgc
     4441 cagaatgaac actgcctcac caattttgac cttgctgagt atcggcaagt actgagtgac
     4501 ttggccattc agatctatca gcagcttgtg agggtgttag agaacattct tcagccaatg
     4561 atagtctcag gcatgctaga gcatgaaaca attcagggag tatctggggt gaagcccaca
     4621 ggactcagaa agcgaacctc cagtatcgcc gatgagggca cctacacact ggactccatc
     4681 ctgcggcagc tcaactcctt ccattccgtc atgtgtcagc atggcatgga cccagagcta
     4741 atcaagcagg tggtcaagca gatgttctac atcgtgggcg ccatcaccct gaacaacctc
     4801 ctgctgcgca aggacatgtg ctcctggagc aaaggcatgc agatacggta caatgtcagt
     4861 caattggaag aatggctacg tgacaagaat ctaatgaaca gtggggcaaa agagactctg
     4921 gaacctctta tccaggctgc tcagcttttg caagtgaaaa agaaaactga cgatgacgcg
     4981 gaagccatct gctccatgtg caacgctctg accacagccc agatcgtcaa agtgttgaat
     5041 ctgtacacac ccgttaatga gtttgaggaa agggtctctg tttcatttat ccgcactata
     5101 caggtgcggt tacgagacag gaaagactct ccacagctgc tcatggatgc aaaacacatc
     5161 tttcctgtca ctttcccctt taacccatcc tccctggccc tagaaaccat ccagattcca
     5221 gccagcctgg gcctgggctt cattgcaagg gtctgaaggg ctaccgaatt cattgatcat
     5281 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5341 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccccgg
     5521 gtaccgagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     5581 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5641 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5701 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5761 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6361 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7681 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     7741 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     7801 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     7861 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     7921 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     7981 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     8041 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     8101 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     8161 gtgccaagtt gggatctttg cattggccca cggctctcag gatggggatg ctccccttca
     8221 gcacccggtt ccccttggaa actgatggtc ctggctctgt ggcatggcag tggcactgtg
     8281 aggagccccc taccagcagc acacagtggg gttggcactg ccacgctccg gatgccgcgc
     8341 tctgatccaa ccccataatc aagggaaccc gaattgcccc atcattgccc ccaccacccc
     8401 catcctgccg ggccctcaca ccccacgctg ccttctggtg acattcccca gcccaaaccc
     8461 acggcttcat ggctaccgcg gggcatttcc cattgccgcc ccattatcag ctctgcacac
     8521 ctcccgctgt acccatgcct cgtggctgcc cttctttgac gtataatctt ctaattaata
     8581 cccggccttg tcaaagtgga gcacaaacgt taattaattc cccagcaggc aggtaattaa
     8641 cagtgtgact ccctttttgc tgcgagtggg gctgatacag agagatgtgg cactatggag
     8701 cccacggggt cctggcactg ggtgcccacg gaggtcccca tgtgctgcag tgtcaccgcc
     8761 tccgaggaga cagtattgtc cctgcggtgt ccctgcagct cagctctgtc cacagggcca
     8821 cctccagttt ggaggggaca caatgcagcc ccgatgcaac ccatcctcgc agcatcccag
     8881 ggacaaagac cccactgcaa gaccgcacac agggctgggt cccgctcccc taatatctac
     8941 agtgcttctt gcatggcccc ttaatcaatg cagttaatca gcatgcgctc atgcaccgct
     9001 ctggagctgc aaagcccctc gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag
     9061 cctgcagcac gcgctgcaaa caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc
     9121 attcggctgc cttgacctcc gccgggccgg gccctgcctg actcagctcc ttactcagcg
     9181 ctcgcttcct ccctccggct gccaccgccg cagcgcacac cctgacaaag agtggccctt
     9241 aacgggctct gaggtgcacc cagcagtgca ctcagcagtc caagggccgg cctggaggtt
     9301 tgcaccgcta cgtgctgaca ttagcattga acttggccct gggtagtgct gcaggccggg
     9361 cggggtgggt gtagagagtg cagcgcgcgt tgcacccggt gccccttccc ctcccttgca
     9421 tcccagcagg ctgcacccca gcaccaggcc cgtgcatgca tgctcctggt gttattgcag
     9481 cctggtgcat gcatgcgtct tgatggtgca gcgctgtgca tgcatcctcc ttggtgtgta
     9541 gcagt
//
