LOCUS       sequence-329942-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUAS::CFP, express CFP when co-express with QF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC812
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3147..3169)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3269..3288
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3366..3384)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3452..3556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3557..3625,3626..4417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3775..3794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac
     1021 atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
     1081 actcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgcat gcccaattcg
     1141 gatccgggta atcgcttatc ctcggataaa caattatcct cacgggtaat cgcttatccg
     1201 ctcgggtaat cgcttatcct cgggtaatcg cttatcctta agcttgatat ctctaggatc
     1261 gattttttgc aaattacgag cgttgtaggg ggcggagcga taggtcctat aggttttggt
     1321 atatcatcat tcattcattc attggtacat tcatttaccc accttcctct ttctgagctt
     1381 ctctggagtt ctgtgcttcc tttttccctt atctttatac tgtaattttt aactttcagc
     1441 cggtagaaaa aatggtttct aagggagaag aacttttcac tggagttgtt ccaattcttg
     1501 ttgaattgga tggagatgtt aacggacata agttctcagt ttctggagaa ggagaaggag
     1561 atgctacata cggaaaactt actttgaagt tcatctgtac aactggaaaa ttgccagttc
     1621 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     1681 cacttgttac aactttgact tggggagttc aatgctttgc aagataccca gatcatatga
     1741 agcaacatga tttctttaag tcagctatgc cagaaggata cgttcaagaa agaacaattt
     1801 tctttaagga tgatggaaac tacaagacac gagcagaggt taagttcgaa ggagatactc
     1861 ttgttaacag aatcgaattg aagggaatcg attttaaaga agatggaaac atccttggac
     1921 ataagttgga atacaatgct atctctgata acgtttacat cactgcagat aagcaaaaga
     1981 atggaattaa agctaacttc aagattcgac ataatattga agatggatca gttcaacttg
     2041 ctgatcatta ccaacaaaac acaccaattg gagatggacc agttcttttg ccagataacc
     2101 attacctttc aactcaatct aagttgtcaa aggatccaaa cgaaaagaga gatcatatgg
     2161 ttcttttgga attcgttaca gctgcaggaa ttactcttgg aatggatgaa ttgtataaat
     2221 aagcattcgt agaattccaa ctgagcgccg gtcgctacca ttaccaactt gtctggtgtc
     2281 aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac taactaacga
     2341 gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
     2401 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
     2461 aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     2521 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     2581 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     2641 tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca cacaaaatac
     2701 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     2761 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     2821 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg gtttccccta
     2881 ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc
     2941 acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa
     3001 gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca
     3061 ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc
     3121 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     3181 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     3241 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     3361 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3421 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3541 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3601 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3661 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3721 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3781 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3841 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3901 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3961 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4021 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4081 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4141 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4201 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4261 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4321 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4381 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4441 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4501 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4561 ccccgtagaa aagatcaaag gatcttc
//
