LOCUS       sequence-329945-        4836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRE::YFP, inducible YFP expression when co-express with rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC811
FEATURES             Location/Qualifiers
     source          1..4836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1153..1171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1189..1207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1225..1243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1261..1279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1297..1315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1333..1351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1369..1387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(1633..1661)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2409..2429
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3396..3418)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3518..3537
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3615..3633)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3701..3805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3806..3874,3875..4666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4024..4043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt aagtttaaac
     1021 atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat ggctgaaatc
     1081 actcacaacg atggatacgc taacaacttg gaaatgaaat aagcttgcat gcccgtcttc
     1141 actcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     1201 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     1261 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     1321 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     1381 atagagaacg tatgtcgagg taggcgtgat cgattttttg caaattacga gcgttgtagg
     1441 gggcggagcg ataggtccta taggttttgg tatatcatca ttcattcatt cattggtaca
     1501 ttcatttacc caccttcctc tttctgagct tctctggagt tctgtgcttc ctttttccct
     1561 tatctttata ctgtaatttt taactttcag ccggtagaaa aaatgagtaa aggagaagaa
     1621 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1681 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     1741 atttgcacta ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact
     1801 aaccctgatt atttaaattt tcagccaaca cttgtcacta ctttcggtta tggtcttcaa
     1861 tgcttcgcca gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
     1921 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
     1981 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc
     2041 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     2101 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     2161 atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact
     2221 aactaatctg atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc
     2281 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     2341 ccagacaacc attacctgtc ctaccaatct gccctttcga aagatcccaa cgaaaagaga
     2401 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     2461 ctatacaaat agcattcgta gaattccaac tgagcgccgg tcgctaccat taccaacttg
     2521 tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact gagttctact
     2581 aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga cttctaagtc
     2641 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     2701 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     2761 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     2821 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     2881 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
     2941 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
     3001 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
     3061 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
     3121 tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta
     3181 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
     3241 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga
     3301 cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc gtacgggccc
     3361 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3421 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3481 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3541 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3601 aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3661 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3841 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3901 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3961 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4021 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4081 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4141 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4201 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4261 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4321 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4381 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4801 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
