LOCUS       sequence-329968-        6576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of mCherry and EP300-C-IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: pmCherry-C-EP300
FEATURES             Location/Qualifiers
     source          1..6576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             636..1343
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(781..799)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1049..1068
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3364..3485
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3401..3420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3455..3474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3492..3947)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3629..3650)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3841..3860
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3974..4078
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4080..4437
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4080..4271
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4103..4123)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4288..4423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4472..5266
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4472..5266
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4526..4545)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5136..5155
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5454..5473)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5498..5545
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5874..6462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6363..6382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcgg ccaccatggt gagcaagggc gaggaggata
      661 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      721 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      781 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      841 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      901 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      961 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1021 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1081 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1141 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     1201 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     1261 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1321 gcggcatgga cgagctgtac aagggtgcgc cgggcagcgc aggcagcgct gcgggtggca
     1381 gcggccactg ccaggagaac aaatgcccgg tgccgttctg cctaaacatc aagcagaagc
     1441 tccggcagca acagctgcag caccgactac agcaggccca aatgcttcgc aggaggatgg
     1501 ccagcatgca gcggactggt gtggttgggc agcaacaggg cctcccttcc cccactcctg
     1561 ccactccaac gacaccaact ggccaacagc caaccacccc gcagacgccc cagcccactt
     1621 ctcagcctca gcctacccct cccaatagca tgccacccta cttgcccagg actcaagctg
     1681 ctggccctgt gtcccagggt aaggcagcag gccaggtgac ccctccaacc cctcctcaga
     1741 ctgctcagcc accccttcca gggcccccac ctgcagcagt ggaaatggca atgcagattc
     1801 agagagcagc ggagacgcag cgccagatgg cccacgtgca aatttttcaa aggccaatcc
     1861 aacaccagat gcccccgatg actcccatgg cccccatggg tatgaaccca cctcccatga
     1921 ccagaggtcc cagtgggcat ttggagccag ggatgggacc gacagggatg cagcaacagc
     1981 caccctggag ccaaggagga ttgcctcagc cccagcaact acagtctggg atgccaaggc
     2041 cagccatgat gtcagtggcc cagcatggtc aacctttgaa catggctcca caaccaggat
     2101 tgggccaggt aggtatcagc ccactcaaac caggcactgt gtctcaacaa gccttacaaa
     2161 accttttgcg gactctcagg tctcccagct ctcccctgca gcagcaacag gtgcttagta
     2221 tccttcacgc caacccccag ctgttggctg cattcatcaa gcagcgggct gccaagtatg
     2281 ccaactctaa tccacaaccc atccctgggc agcctggcat gccccagggg cagccagggc
     2341 tacagccacc taccatgcca ggtcagcagg gggtccactc caatccagcc atgcagaaca
     2401 tgaatccaat gcaggcgggc gttcagaggg ctggcctgcc ccagcagcaa ccacagcagc
     2461 aactccagcc acccatggga gggatgagcc cccaggctca gcagatgaac atgaaccaca
     2521 acaccatgcc ttcacaattc cgagacatct tgagacgaca gcaaatgatg caacagcagc
     2581 agcaacaggg agcagggcca ggaataggcc ctggaatggc caaccataac cagttccagc
     2641 aaccccaagg agttggctac ccaccacagc agcagcagcg gatgcagcat cacatgcaac
     2701 agatgcaaca aggaaatatg ggacagatag gccagcttcc ccaggccttg ggagcagagg
     2761 caggtgccag tctacaggcc tatcagcagc gactccttca gcaacagatg gggtcccctg
     2821 ttcagcccaa ccccatgagc ccccagcagc atatgctccc aaatcaggcc cagtccccac
     2881 acctacaagg ccagcagatc cctaattctc tctccaatca agtgcgctct ccccagcctg
     2941 tcccttctcc acggccacag tcccagcccc cccactccag tccttcccca aggatgcagc
     3001 ctcagccttc tccacaccac gtttccccac agacaagttc cccacatcct ggactggtag
     3061 ctgcccaggc caaccccatg gaacaagggc attttgccag cccggaccag aattcaatgc
     3121 tttctcagct tgctagcaat ccaggcatgg caaacctcca tggtgcaagc gccacggacc
     3181 tgggactcag caccgataac tcagacttga attcaaacct ctcacagagt acactagaca
     3241 tacactaggc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     3301 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3361 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3421 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3481 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     3541 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3601 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3661 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3721 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3781 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3841 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3901 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     4081 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4141 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4261 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4321 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4381 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     4441 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     4501 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4561 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4621 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4681 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4741 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4801 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4861 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4921 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4981 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5041 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5101 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5161 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5221 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     5281 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     5341 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5401 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     5461 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     5521 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     5581 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     5641 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5701 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6001 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6541 cctgattctg tggataaccg tattaccgcc atgcat
//
