LOCUS       sequence-329971-        8136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length SRGAP1 with a C-terminal Myc fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Coutinho-Budd J, Ghukasyan V, Zylka MJ, Polleux F
  TITLE     The F-BAR domains from srGAP1, srGAP2 and srGAP3 regulate membrane 
            deformation differently.
  JOURNAL   J Cell Sci. 2012 Jul 15;125(Pt 14):3390-401. doi: 
            10.1242/jcs.098962. Epub 2012 Mar 30.
  PUBMED    22467852
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-07-15"; volume: "125(Pt 14):3390-401. doi"; 
            pages: " 10.1242/jcs.098962. Epub 2012 Mar 30"
COMMENT     Sequence Label: pCAG-SRGAP1-Myc
FEATURES             Location/Qualifiers
     source          1..8136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(801..1592,1593..1661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1424..1443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1662..1766)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1797..2176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2178..2455
                     /label=chicken beta-actin promoter
     intron          2456..3472
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3396..3418
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3480..3499
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6797..6826
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6920..6939)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6985..7040
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7039..7058)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7431..7447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7431..7447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7444..7466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7455..7471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7479..7485,7486..7503,7504..7509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7524..7545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7604..7799
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7650..7785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7805..7939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7842..7861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7896..7915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8007..8024)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     1801 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2161 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2221 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2281 ggcggggggg gggggggggc gcgcgccrgg sggggsgggg sggggsgrgg ggsggggsgg
     2341 ggsgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     2401 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     2461 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     2521 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     2581 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     2641 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     2701 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     2761 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     2821 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     2881 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     2941 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     3001 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
     3061 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     3121 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     3181 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     3241 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     3301 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     3361 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     3421 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     3481 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgctcg agataatgtc
     3541 caccccgagc cgattcaaga aggacaaaga gatcatagcc gagtatgaaa gtcaagtcaa
     3601 agaaattcga gctcaactgg tagaacaaca aaaatgcctg gagcagcaaa cggagatgcg
     3661 agttcagctt ctccaggatc tgcaagattt cttccgaaaa aaagctgaaa ttgagacgga
     3721 atattcccgg aatctagaga agttagcaga aaggttcatg gcaaaaacaa gaagcactaa
     3781 ggatcatcaa caatacaaga aagaccagaa cctgttgtct ccagtgaact gctggtattt
     3841 gctcctgaac caagtaagga gagaaagcaa agaccatgca accttgagtg acatctatct
     3901 gaacaatgtg attatgcggt tcatgcagat aagtgaggat tctaccagga tgtttaaaaa
     3961 gagcaaagag attgcattcc aacttcatga ggatttaatg aaggttctta atgagcttta
     4021 tacggtgatg aaaacatacc atatgtatca tgcagagagc atcagtgcag agagcaagct
     4081 gaaagaggcc gaaaaacaag aggaaaagca aattgggaga tctggtgatc cagtcttcca
     4141 tattcgacta gaggagagac atcaacggcg aagctctgta aagaaaattg aaaaaatgaa
     4201 agaaaaaaga caagcaaaat attcagaaaa taagctaaaa tcaattaagg cacggaacga
     4261 atatctccta acacttgaag ccaccaatgc ctcagttttc aagtactata ttcatgatct
     4321 ttctgattta attgattgct gtgatcttgg ctaccatgca agtctgaaca gagccctaag
     4381 aacatatctg tctgcggagt acaaccttga aacctccaga catgagggct tagacattat
     4441 tgagaatgca gttgataatt tagagcccag gagcgataag cagagattca tggagatgta
     4501 ccctgctgcg ttctgtccac caatgaagtt tgagtttcag tctcacatgg gtgatgaggt
     4561 gtgccaggtc agtgcccagc agccagtcca ggcagagctc atgctcaggt accaacagtt
     4621 gcagtcccgc cttgccacgc tcaaaatcga gaatgaagag gttaagaaaa cgactgaagc
     4681 caccttgcag acgatacaag atatggtcac catcgaggac tatgatgttt ctgaatgctt
     4741 ccagcacagt cgttccacag aatctgtgaa gtccactgtc tctgaaacct acctgagtaa
     4801 acccagcatc gccaagagaa gagccaacca gcaggaaact gaacagttct acttcatgaa
     4861 actcagagaa tatttggaag gcagtaatct catcacaaaa cttcaagcca aacatgactt
     4921 gctgcagagg accctgggag aaggtcatag agctgaatat atgactacaa ggcctccaaa
     4981 tgttccccct aagccccaga aacacaggaa gtccaggccc cgctcacagt ataatactaa
     5041 gttgtttaat ggggatttgg aaacattcgt caaggactca ggacaggtta ttcccctcat
     5101 tgtggaaagc tgtattcggt tcatcaatct ctatggtctt cagcatcagg ggattttcag
     5161 agtgtctggt tcccaggtgg aagtcaatga tattaaaaat tcatttgaga gaggtgaaaa
     5221 tcctttggct gatgaccaga gtaaccatga tattaactca gttgctggcg ttctgaagct
     5281 ctatttccgt gggctggaaa accccctctt tcctaaggaa agatttaacg atctgatttc
     5341 ttgtatcaga atagataatc tctatgagag ggcgcttcac atccgcaaac tcctcctgac
     5401 tttgcccagg tcggtcctta tagtgatgag gtacctcttt gccttcctca atcatctatc
     5461 acagtacagc gatgagaata tgatggaccc ttataacctg gccatttgct ttggcccaac
     5521 attgatgcct gtcccagaaa tacaggatca agtgtcttgc caggcacatg tgaatgaaat
     5581 tatcaaaacc atcatcatcc accatgagac tattttccca gatgctaaag agctggatgg
     5641 ccctgtttat gagaaatgta tggctggaga tgactattgc gacagcccat acagtgagca
     5701 cggtacattg gaggaagtgg accaagatgc tggtacagag ccccacacaa gtgaagatga
     5761 atgtgagcca atagaagcaa tagccaagtt tgactatgtt gggcggtccg ccagagaact
     5821 atccttcaag aagggtgcct ccctgctgct gtatcaccgt gcatctgagg actggtggga
     5881 aggcaggcac aacgggattg acgggctggt gcctcaccag tatatagtgg tgcaggatat
     5941 ggatgatacg ttttcagaca ctctgagcca aaaagccgac agtgaggcca gcagtgggcc
     6001 agtcacggaa gacaagtcct catccaagga catgaactcc ccgacagacc gtcatcctga
     6061 cggctattta gccaggcaac gaaaaagagg agagccaccc cctccagtaa ggcgtcctgg
     6121 caggaccagt gatggccatt gcccgctcca ccctccacat gccctttcta actcctcagt
     6181 tgacctaggg tccccaagcc ttgccagtca cccccggggc ctgctgcaga accgtggcct
     6241 caacaatgac agtcctgagc ggaggcgcag gcctggccat ggcagcctga ccaacatcag
     6301 ccggcacgac tccctcaaga agatcgacag ccctcccatt agaaggtcca cgtcatcagg
     6361 gcaatacacg ggcttcaatg accacaagcc actggaccca gagacaattg ctcaggatat
     6421 tgaagaaacg atgaacacag ctttgaatga actccgagaa ctggagagac agagcacagc
     6481 aaagcatgcc cctgatgtgg tgctggatac cctggagcaa gtgaaaaact ctcccacccc
     6541 tgccacttcc acggaatctc tcagcccttt gcacaacgtt gccctcagga gctccgagcc
     6601 tcagattcga cgtagcacga gctcctccag tgacacaatg agtactttca agcctatggt
     6661 ggcacccaga atgggcgtgc agctgaagcc tccagccctt aggccaaaac ctgctgttct
     6721 tccaaaaaca aatcctacca taggacctgc cccacctccc cagggtccaa cagacaagtc
     6781 atgcacaatg gctagcgaac aaaaactcat ctcagaagag gatctgtaag cggccgcaat
     6841 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     6901 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6961 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     7021 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     7081 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     7141 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     7201 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     7261 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     7321 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcacc
     7381 gtcgaccagc tggtcgacgg tgcaccgtcg accagcttgg cgtaatcatg gtcatagctg
     7441 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7501 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7561 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     7621 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7801 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7921 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7981 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8041 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8101 gataacgcag gaaagaacat gtgagcaaaa ggccag
//
