LOCUS       sequence-32999-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Benink HA, Bement WM
  TITLE     Concentric zones of active RhoA and Cdc42 around single cell wounds.
  JOURNAL   J Cell Biol. 2005 Jan 31. 168(3):429-39.
  PUBMED    15684032
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 Jan 31. 168(3):429-39."
COMMENT     Sequence Label: mRFP-wGBD
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    126..260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(163..182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     217..236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(632..655)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ccctcactaa agggaacaaa agctgggtac cgggcccaat gcattggcgc cgcggccgcg
       61 aattaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      241 tatcttatca tgtctggatc tacgtaatac gactcactat agttctagaa ctcctggcgc
      301 ctcatctcct gccgcacagc ctccagccca ccctggtcct caatgaagtc gtagataagt
      361 ttagaggtct cggcgtcggt gagctgggcc tcgctgattc ctgccctgga gaacagactc
      421 cgcagatctg ggtcgaggtt gttcacgtca aatccattct gggggtccca ccccacgtgg
      481 ctgacatgct tgaatccact gggtgcacca atatcagctt tgctgatctt cttcttccct
      541 gagcgtttct tatcagctgg gctaggtccg gaggcgccgg tggagtggcg gccctcggcg
      601 cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttgatg
      661 tcggtcttgt ag
//
