LOCUS       sequence-33003-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Benink HA, Bement WM
  TITLE     Concentric zones of active RhoA and Cdc42 around single cell wounds.
  JOURNAL   J Cell Biol. 2005 Jan 31. 168(3):429-39.
  PUBMED    15684032
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 Jan 31. 168(3):429-39."
COMMENT     Sequence Label: GFP-pGBD
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..132)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(112..130)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(151..167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(151..167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(164..186)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    175..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(199..205,206..223,224..229))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    244..265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(382..399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(633..652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
      121 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      181 tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
      241 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      361 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtcca
//
