LOCUS       sequence-330032-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CHD7 (K999R) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7 (K999R)
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..136
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      478..487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggattattc ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc
       61 aaaggcctac gtcgacaaga tgcatcatca tcatcatcac ggtggtggtg gtggtgaaaa
      121 cctgtacttc cagggaatgg cagatccagg aatgatgagt ctttttggcg aggatgggaa
      181 tattttcagt gaaggtcttg aaggcctcgg agaatgtggt tacccggaaa atccagtaaa
      241 tcctatgggt cagcaaatgc caatagacca aggctttgcc tctttacagc catcccttca
      301 tcatccttca actaatcaaa atcaaacaaa gctgacacat tttgatcact ataatcagta
      361 tgaacaacaa aagatgcatc tgatggatca gccgaacaga atgatgagca acacccctgg
      421 gaacggactc gcgtctccgc actcgcagta tcacacccct cccgttcctc aggtgcccca
      481 tggtggcagt ggtggcggtc agatgggtgt ctaccctggc atgcagaatg agaggcatgg
      541 gcaatccttt gtggacagca gctccatgtg gggccccagg gctgttcagg taccagacca
      601 gatacgagcc ccctaccagc agcagcagcc acagccgcag ccaccgcagc cggctccgtc
      661 ggggccccct gcacagggcc accctcagca catgcagcag atgggcagct atatggcacg
      721 tggggatttt tccatgcagc agcatggtca gccacagcag
//
