LOCUS       sequence-330053-        2577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6-driven sgRNA targeting LMNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Xu H, Wang J, Liang Y, Fu Y, Li S, Huang J, Xu H, Zou W, Chen B
  TITLE     TriTag: an integrative tool to correlate chromatin dynamics and gene
            expression in living cells.
  JOURNAL   Nucleic Acids Res. 2020 Oct 26. pii: 5940501. doi: 
            10.1093/nar/gkaa906.
  PUBMED    33104788
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 26. pii: 5940501. doi: 10.1093/nar/gkaa906."
COMMENT     Sequence Label: PHY55-mouse U6-sgLMNA
FEATURES             Location/Qualifiers
     source          1..2577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    complement(455..488)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     492..512
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     rep_origin      complement(822..1410)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(902..921)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1581..2372,2373..2441))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2204..2223
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2442..2546)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacgtt
      241 agtaccgggc ccgctctaga gatccgacgc gccatctcta ggcccgcgcc ggccccctcg
      301 cacggacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt
      361 tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata
      421 ctagctacat tttacatgat aggcttggat ttctataact tcgtatagca tacattatac
      481 gaagttataa acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga
      541 gactataagt atcccttgga gaaccacctt gttggccatg gagaccccgt cccaggttta
      601 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
      661 aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt
      721 ggataaccgt attacgggct aattcactcc caacgaactc acatgtgagc aaaaggccag
      781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      841 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      901 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      961 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1021 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1081 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1141 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1201 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1261 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1321 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1381 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1441 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1501 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1561 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1621 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1681 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     1741 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1801 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1861 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1921 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1981 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2041 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2101 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2161 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2221 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2281 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2341 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2401 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2461 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2521 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
