LOCUS       sequence-330057-        5182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast display plasmid for expression of C-terminal fusions with 
            Aga2p yeast agglutinin. The reporter DnbALFA was fused to the 
            N-terminus of Aga2p..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDC3
FEATURES             Location/Qualifiers
     source          1..5182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(164..838)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(839..1119)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(1140..1159)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1220..1238)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1279..1783
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        1820..1924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1925..2740
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1925..2740
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1998..2017)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2911..3499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3400..3419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3682..4123
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3682..3799
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4094..4117
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4905..4916
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4917..4943
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5074..5103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 caatatactt ttcatttctc cgtaaacaac atgttttccc atgtaatatc cttttctatt
       61 tttcgttccg ttaccaactt tacacatact tttgcggtat ttcacaccgc atagatcggc
      121 aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc ttagcatttt
      181 tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta
      241 catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc
      301 caccagctaa cataaaatgt aagctttcgg ggctctcttg ccttccaacc cagtcagaaa
      361 tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac aagggaataa
      421 acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt ggaaatacga
      481 gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac tcatctccat
      541 gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc tccttaggtt
      601 gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta tatgctttta
      661 caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta ttgggcacac
      721 atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc tctgtgctct
      781 gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata acagacatac
      841 tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt caccaatgcc
      901 ctccctcttg gccctctcct tttctttttt cgaccgaatt aattcttaat cggcaaaaaa
      961 agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc tatttttcaa taaagaatat
     1021 cttccactac tgccatctgg cgtcataact gcaaagtaca catatattac gatgctgttc
     1081 tattaaatgc ttcctatatt atatatatag taatgtcgtg atctatggtg cactctcagt
     1141 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1201 gcgcccttga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1261 ataatggttt cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat
     1321 gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
     1381 ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa aataaacaaa
     1441 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
     1501 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
     1561 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
     1621 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
     1681 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
     1741 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
     1801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1921 gagtatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
     1981 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     2041 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     2101 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     2161 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     2221 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     2281 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     2341 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     2401 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     2461 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     2521 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     2581 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     2641 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     2701 taaattgcag tttcatttga tgctcgatga gtttttctaa ctgtcagacc aagtttactc
     2761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccac ctacatacct
     3121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3481 ggggcggagc ctatgaaaaa acgccagcaa cgcggcactt tgtaactgag ctgtcatttt
     3541 gctggcccag ctggtcgacc gcggctaagc agtactggta ccgttgtgga aatgtaaaga
     3601 gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat acgcttaact
     3661 gctcattgct atattgaagt acggattaga agccgccgag cgggtgacag ccctccgaag
     3721 gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc
     3781 ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa
     3841 gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atgaacgaat caaattaaca
     3901 accataggat gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga
     3961 agcgatgatt tttgatctat taacagatat ataaatgcaa aaactgcata accactttaa
     4021 ctaatacttt caacattttc ggtttgtatt acttcttatt caaatgtaat aaaagtatca
     4081 acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac cccggatcga
     4141 attccctact tcatacattt tcaattaaga tgaggttccc atctattttc accgctgttg
     4201 tttttgctgc ttcttctgct ttggctgctc cagctaatgg tatgagcggt gaagttcagc
     4261 tgcaagaaag cggtggtggt ctggttcagc ctaatggtag cctgcgtctg agctgtaacg
     4321 caagcggtgt taccattagc gcactgaatg caatggcaat gggttggtat cgtcaggcac
     4381 cgggtgaacg tcgtgttatg gttgcagcag ttagcgaacg tggtaatgca atgtatcgtg
     4441 aaagcgttaa aggtcgtttt accatttctc gtgataatgc taacaatatg gttagcctgc
     4501 agatggataa tctgaaaccg gaagataccg cagtgtatta ttgtcatgtt ctggaagatc
     4561 gtggtgatac ttttcatgat tattggggtc agggcaccca ggttaccgtt agcagctctg
     4621 gtggaggtgg ttcgggcgga ggtggtaacg gatctaatgg ttctggcggt agctctgcac
     4681 aggaactgac aactatatgc gagcaaatcc cctcaccaac tttagaatcg acgccgtact
     4741 ctttgtcaac gactactatt ttggccaacg ggaaggcaat gcaaggagtt tttgaatatt
     4801 acaaatcagt aacgtttgtc agtaattgcg gttctcaccc ctcaacaact agcaaaggca
     4861 gccccataaa cacacagtat gtttttaagg acaatagctc gacgattgaa ggtagatacc
     4921 catacgacgt tccagactac gctctgcagg ctagtggtgg aggaggctct ggtggaggcg
     4981 gtagcggagg cggagggtcg gctagccata tgcacgatga actataatga agcatgctta
     5041 attaaggcgc cttatctcga ggggggcgga tccgaacaaa agcttatttc tgaagaggac
     5101 ttgtaataga gatctgataa caacagtgta gatgtaacaa aatcgacttt gttcccactg
     5161 tacttttagc tcgtacaaaa ta
//
