LOCUS       sequence-330063-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. 
            Plasmid for lentivirus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Webb BA, Aloisio FM, Charafeddine RA, Cook J, Wittmann T, Barber DL
  TITLE     pHLARE: A New Biosensor Reveals Decreased Lysosome pH in Cancer 
            Cells.
  JOURNAL   Mol Biol Cell. 2020 Nov 25:mbcE20060383. doi: 
            10.1091/mbc.E20-06-0383.
  PUBMED    33237838
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Nov 25:mbcE20060383. doi: 10.1091/mbc.E20-06-0383."
COMMENT     Sequence Label: pLenti sfGFP-LAMP1-mCherry (pHLARE)
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        189..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     647..667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     693..717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             780..869
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             870..1580
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGYTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(909..930)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1517..1538
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2811..2820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2817..3527
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2962..2980)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3230..3249
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3562..3582)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3567..3896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3747..3882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3944..3991
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4010..4408
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4495..4728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4800..4921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4837..4856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4891..4910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4961..5096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5116..5135)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5117..5135)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5145..5162)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5145..5161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5154..5176)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5303..5758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5390..5409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5600..5621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5784..5888
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5889..5957,5958..6749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6107..6126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6920..7508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7409..7428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7662..7679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7796..7817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7832..7837,7838..7855,7856..7862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7870..7886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7875..7897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7894..7910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7894..7910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7929..7949
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7931..7949
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7977..8203
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8204..8384
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8431..8556
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9049..9282
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
       61 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
      121 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ataagcttgg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      721 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatccaa
      781 tggacagcaa aggttcgtcg cagaaagggt cccgcctgct cctgctgctg gtggtgtcaa
      841 atctactctt gtgccagggt gtggtctcca gcaagggcga ggagctgttc accggggtgg
      901 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg
      961 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     1021 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1081 gccgctaccc cgaccacatg aagcgccacg acttcttcaa gtccgccatg cccgaaggct
     1141 acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc cgcgccgagg
     1201 tgaagttcga gggctacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1261 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
     1321 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacgtgg
     1381 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1441 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc aaagacccca
     1501 acgagaagcg cgaccacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg
     1561 gcatggacga gctgtacaag tccggatcga gcgccaccgc cccagcactg ttcgaggtga
     1621 aagacaacaa cggcacagcg tgtataatgg ccagcttctc tgcctccttt ctgaccacct
     1681 atgaggctgg acatgtttct aaggtctcga atatgaccct gccagcctct gcagaagtcc
     1741 tgaagaatag cagctcttgt ggtgaaaaga atgcttctga gcccaccctc gcaatcacct
     1801 ttggagaagg atatttactg aaactcacct tcacaaaaaa cacaacacgt tacagtgtcc
     1861 agcacatgta tttcacatat aacctgtcag acacacaatt ctttcccaat gccagctcca
     1921 aagggcccga cactgtggat tccacaactg acatcaaggc agacatcaac aaaacatacc
     1981 gatgtgtcag cgacatcagg gtctacatga agaatgtgac cattgtgctc tgggacgcta
     2041 ctatccaggc ctacctgccg agtagcaact tcagcaagga agagacacgc tgcccacagg
     2101 atcaaccttc cccaactact gggccaccca gcccctcacc accacttgtg cccacaaacc
     2161 ccagtgtgtc caagtacaat gtgactggtg acaatggaac ctgcctgctg gcctctatgg
     2221 cactgcaact caacatcacc tacatgaaga aggacaacac gactgtgacc agagcattca
     2281 acatcaaccc aagtgacaaa tatagtggga cttgcggtgc ccagttggtg accctgaagg
     2341 tggggaacaa gagcagagtc ctggagctgc agtttgggat gaatgccact tctagcctgt
     2401 ttttcctgca aggagttcag ttgaacatga ctcttcctga tgccatagag cccacgttca
     2461 gcacctccaa ctattccctg aaagctcttc aggccagtgt cggcaactca tacaagtgca
     2521 acagtgagga gcacatcttt gtcagcaagg cgctcgccct caatgtcttc agcgtgcaag
     2581 tccaggcttt cagggtagaa agtgacaggt ttgggtctgt ggaagagtgt gtacaggacg
     2641 gtaacaacat gctgatcccc attgctgtgg gcggggccct ggcagggctg gtcctcatcg
     2701 tcctcatcgc ctatctcatc ggcaggaaga ggagtcacgc gggctatcag accatctcgg
     2761 aattcggctc caccggctcc accggctcca ccggcgcgga tccaccggtc gccaccatgg
     2821 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     2881 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2941 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     3001 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     3061 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     3121 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     3181 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     3241 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     3301 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     3361 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     3421 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     3481 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaatac cggttagtaa
     3541 tgagtttgga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3601 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3721 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3781 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     3841 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     3901 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     3961 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc
     4021 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     4081 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     4141 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     4201 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     4261 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     4321 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     4381 gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa
     4441 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4501 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4561 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4621 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4681 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     4741 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4921 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5041 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5101 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5161 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5221 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5281 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5341 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5401 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5461 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5521 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5581 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5641 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5701 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5761 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5881 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5941 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6001 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6061 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7801 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7861 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7921 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7981 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     8041 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8101 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8161 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8221 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8281 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8341 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8401 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8461 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8521 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8581 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8641 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8701 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8761 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8821 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8881 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8941 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9001 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9061 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9121 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9181 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9241 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9301 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9361 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9421 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga attaacaaga
     9481 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9541 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9601 t
//
