LOCUS       sequence-330072-        2355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry 
            under ColE promoter with a LexA408 operator in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pLEVI(408)-ColE-iLight-msfGFP
FEATURES             Location/Qualifiers
     source          1..2355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1636..1638,1639..1641,1642..2355)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1681..1702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2289..2310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgctggtg gggcagcaga tctgggtagt gatgatatca gcaaactgat tgcagcatgt
       61 gatcaagaac cgattcatat tccgaatgca attcagccgt ttggtgcaat gctgattgtt
      121 gaaaaagata cccagcagat tgtttatgca agcgcaaata gcgcagaata tttcagcgtt
      181 gcagataaca ccattcatga actgagcgat tttaaacagg ccaacattaa tagcctgctg
      241 ccggaacaac tgattagcgg tctgacaagc gcaattagtg aaaatgaacc gatttgggtt
      301 gaaaccgatc gtctgagctt tctgggttgg cgtcatgaaa actattacat cattgaagtg
      361 gaacgctatc atgtgcagac cagcaattgg tttgaaattc agtttcagcg tgcctttcag
      421 aaactgcgta attgcaaaac ccataacgat ctgattaata ccctgacccg tctgattcaa
      481 gaaatcagcg gttatgatcg cgtgatgatc tatcaatttg atccggaatg gaatggtcgt
      541 gttattgcag aaagcgttcg tcagctgttt accagcatgc tgaatcatca ttttccggca
      601 agcgatattc cggcacaggc acgtgcaatg tatagcatta atccgattcg tattatcccg
      661 gatgttaatg cagaaccgca gccgctgcac atgattcata aaccgcaaaa taccgaagca
      721 gttaatctga gctgcggtgt tctgcgtgca gttagccctc tgcacatgca gtatctgcgt
      781 aattttggtg ttagcgcaag caccagcatt ggcattttta acgaagataa actgtggggt
      841 atcgttgcat gccatcatac caaaccgcgt gcaattggtc gtcgtattcg tcatctgctg
      901 gttcgtaccg ttgaatttgc agcagaacgt ctgtggctga ttcatagccg taatgttgaa
      961 cgttatatgg ttaccgttca ggcagcacgt gaacagctga gcaccaccgc agatgataaa
     1021 cattcaagcc atgaaatcgt gattgaacat gcagcagatt ggtgtaaact gtttcgttgt
     1081 gatggtgttg gttatctgcg tggagaagaa ctgaccacct atggtgaaac accggatcag
     1141 accaccatta acaaactggt tgaatggctg gaagagaacg gtaaaaaaag cctgttttgg
     1201 catagccaca tgctgaaaga agatgcaccg ggtctgctgc cggatggtag ccgttttgca
     1261 ggtctgctgg caattccgct gaaaagtgat gcagacctgt ttagctatct gctgctgttt
     1321 cgtgtggcac agaatgaagt tcgtacctgg gcgggtaaac cggaaaaact gagcgttgaa
     1381 accagcactg gcaccatggt gggtccgcgt aaaagttttg aagcatggca ggatgaagtt
     1441 agcggtaaaa gccagccgtg gcgtaccgca cagctgtatg cagcacgtga tattgcacgt
     1501 gatctgctga ttgtggcaga tagcatgcag ctgaatctgc tgaatgatca gctggcagat
     1561 gcaaatgaaa atctggaaaa actggccagc tttgatgatc tgaccgtcga ctccggtggt
     1621 ggttctggtg gtggaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1681 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag
     1741 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1801 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1861 cccgaccaca tgaagcgcca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1921 gagcgcacca tcagcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc
     1981 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2041 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcaccgcc
     2101 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacgt ggaggacggc
     2161 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2221 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagatcc caacgagaaa
     2281 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2341 gagctgtaca agtga
//
