LOCUS       sequence-330078-        2382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct 
            expression in mammalian cells. pAAV vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pAAV-CaMKII-GAL4-iLight-VP16
FEATURES             Location/Qualifiers
     source          1..2382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2146..2379
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 gccaccatgg cgcgcaagaa gaagaggaag tccggaggtg gtggaaagct actgtcttct
       61 atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg
      121 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg
      181 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag
      241 actagtgctg gtgggtccgc cggcgggagt gctggcggta gtgccggcgg tgaattcgcc
      301 gccgacctgg gctctgacga tatcagcaag ctgatcgccg cctgcgatca ggagccaatc
      361 cacatcccca atgccatcca gccatttggc gccatgctga tcgtggagaa ggacacacag
      421 cagatcgtgt acgcctctgc caacagcgcc gagtacttca gcgtggccga caataccatc
      481 cacgagctgt ccgatttcaa gcaggccaac atcaattctc tgctgcccga gcagctgatc
      541 agcggcctga catccgccat ctctgagaac gagcctatct gggtggagac cgacaggctg
      601 agctttctgg gctggcgcca cgagaactac tatatcatcg aggtggagag ataccacgtg
      661 cagacatcca attggttcga gatccagttt cagcgggcct tccagaagct gagaaactgt
      721 aagacccaca acgatctgat caataccctg acacggctga tccaggagat cagcggctac
      781 gacagagtga tgatctatca gttcgatccc gagtggaatg gcagagtgat cgccgagagc
      841 gtgagacagc tgtttacctc catgctgaac caccacttcc cagcctctga catccctgca
      901 caggccaggg ccatgtacag catcaaccca atccgcatca tccccgatgt gaatgccgag
      961 ccccagcctc tgcacatgat ccacaagcca cagaacacag aggccgtgaa tctgtcctgc
     1021 ggcgtgctga gggccgtgtc tccactgcac atgcagtatc tgcgcaactt tggcgtgtct
     1081 gccagcacct ccatcggcat cttcaatgag gacaagctgt ggggcatcgt ggcctgtcac
     1141 cacacaaagc ctagggccat cggccggaga atcaggcacc tgctggtgcg caccgtggag
     1201 tttgcagcag agcgcctgtg gctgatccac tccaggaatg tggagcggta catggtgaca
     1261 gtgcaggcag cccgggagca gctgtctacc acagccgacg ataagcacag ctcccacgag
     1321 atcgtgatcg agcacgccgc cgactggtgc aagctgttcc ggtgtgatgg cgtgggctac
     1381 ctgagaggcg aggagctgac cacatatggc gagacccctg atcagaccac aatcaacaag
     1441 ctggtggagt ggctggagga gaatggcaag aagagcctgt tttggcactc ccacatgctg
     1501 aaggaggacg cacctggact gctgccagat ggcagccggt tcgcaggact gctggccatc
     1561 ccactgaagt ctgacgccga tctgtttagc tacctgctgc tgttcagggt ggcacagaac
     1621 gaggtgcgca catgggcagg caagcctgag aagctgtccg tggagacctc tacaggcacc
     1681 atggtgggcc cacggaagtc ttttgaggcc tggcaggacg aggtgagcgg caagtcccag
     1741 ccttggagaa ccgcacagct gtatgcagcc cgggacatcg cccgggacct gctgatcgtg
     1801 gccgatagca tgcagctgaa cctgctgaat gaccagctgg ccgatgccaa cgagaatctg
     1861 gagaagctgg cctccttcga cgatctgacc tctggcggcg gtaccagcgg gggtggtgga
     1921 tcaggtggag gaggttctgg aggtggtgga tcaggaggag gtggttctgg aggtggtgga
     1981 tcaggaggaa gatctgcgta cagccgcgcg cgtacgaaaa acaattacgg gtctaccatc
     2041 gagggcctgc tcgatctccc ggacgacgac gcccccgaag aggcggggct ggcggctccg
     2101 cgcctgtcct ttctccccgc gggacacacg cgcagactgt cgacggcccc cccgaccgat
     2161 gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac
     2221 gcgctagacg atttcgatct ggacatgttg ggggacgggg attcccctgg tccgggattt
     2281 accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga gtttgagcag
     2341 atgtttaccg atgcccttgg aattgacgag tacggtgggt aa
//
