LOCUS       sequence-330091-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of Necl-5, the plasmid encodes a recombinant 
            Necl-5 fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, 
            Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
  TITLE     Functional visualization of NK Cell-mediated killing of metastatic 
            single tumor cells.
  JOURNAL   Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
  PUBMED    35113018
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-3"; volume: "11. pii: 76269. doi"; pages: " 
            10.7554/eLife.76269"
COMMENT     Sequence Label: pPBbsr2-Necl-5-ScNeo
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1393..1668
                     /label=chicken beta-actin promoter
     intron          1669..2678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2602..2624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2686..2705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2832..3527
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2965..2983)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3233..3252
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5497..6045
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5641..5658)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5868..5887
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6516..6533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6522..6746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6793..6809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6817..6833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6841..6847,6848..6865,6866..6871))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6886..6907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7141..7203
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7244..7264)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7244..7262)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7283..7299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7307..7323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7331..7337,7338..7355,7356..7361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7376..7397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7514..7531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7685..8273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7765..7784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8444..9235,9236..9304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9067..9086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9305..9409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg
     1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1801 cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc
     1861 cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1921 ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     1981 gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2041 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct ccccgagttg
     2161 ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg
     2221 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     2281 gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     2461 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     2521 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     2581 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     2641 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     2701 ttgtgctgtc tcatcatttt ggcaaagaat tgggatccga attcaccatg gctcaactcg
     2761 cccgagccac ccgctccccg ctgtcatggc tgctgctgct gttctgctat gcactccgga
     2821 aagcgggtgg gatggtgagc aagggcgagg cagtgatcaa ggagttcatg cggttcaagg
     2881 tgcacatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     2941 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     3001 tctcctggga catcctgtcc cctcagttca tgtacggctc cagggccttc accaagcacc
     3061 ccgccgacat ccccgactac tataagcagt ccttccccga gggcttcaag tgggagcgcg
     3121 tgatgaactt cgaggacggc ggcgccgtga ccgtgaccca ggacacctcc ctggaggacg
     3181 gcaccctgat ctacaaggtg aagctccgcg gcaccaactt ccctcctgac ggccccgtaa
     3241 tgcagaagaa gacaatgggc tgggaagcgt ccaccgagcg gttgtacccc gaggacggcg
     3301 tgctgaaggg cgacattaag atggccctgc gcctgaagga cggaggccgc tacctggcgg
     3361 acttcaagac cacctacaag gccaagaagc ccgtgcagat gcccggcgcc tacaacgtcg
     3421 accgcaagtt ggacatcacc tcccacaacg aggactacac cgtggtggaa cagtacgaac
     3481 gctccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga ggctcggata
     3541 tacgtgtgct ggtgccctac aattcgacag gcgtcttggg agggtcgacc accttgcact
     3601 gtagtctgac ttctaatgag aatgtgacta tcactcaaat aacctggatg aagaaggatt
     3661 caggtggatc ccacgctctt gtggctgtct tccaccccaa gaaggggccc aacatcaaag
     3721 agccagagag ggtgaaattc ttggctgccc aacaggatct gaggaacgca tctctggcca
     3781 tctcgaactt aagtgtagaa gacgaaggca tctatgaatg tcagattgcc acattcccca
     3841 gaggcagtag aagcaccaat gcctggctga aggtgcaagc ccgacctaag aacactgcag
     3901 aggccctgga gccctctccc accttgatac tgcaggatgt ggctaaatgc atctctgcca
     3961 atggtcaccc tcctggacga atctcttggc cctcgaatgt gaatggaagt caccgtgaaa
     4021 tgaaggaacc agggtcccag ccgggcacca ccacagttac cagctacctc tccatggtac
     4081 cttctcgcca ggcagacggc aagaacatca cctgcacggt ggagcatgaa agcttacagg
     4141 agctggacca gctgctggtg accctttccc aaccctatcc acctgaaaac gtgtccatct
     4201 ctggctatga cggcaactgg tatgttggcc tcactaactt gaccctgacc tgtgaagctc
     4261 acagcaaacc agcgcctgac atggctggat ataactggag cacgaacacg ggtgactttc
     4321 ccaactctgt taagcgccag ggcaatatgc ttctaatctc caccgtagag gatggtctca
     4381 ataacacggt cattgtgtgc gaagtcacca atgccctagg gtctgggcag ggccaagtgc
     4441 acatcattgt taaagagaaa cctgagaata tgcagcaaaa tacaagatta cacctaggct
     4501 acatctttct tatcgtcttt gtcctcgctg tagtcatcat catcgcagca ctatacacta
     4561 tacgaagatg caggcatggt cgtgctctgc agtccaatcc ctcagagagg gagaacgtcc
     4621 agtattcatc tgtgaacggc gactgtagac tgaacatgga gccaaacagc acaaggttcg
     4681 aaatggtaag caagggggag gaagataata tggcttccct tccagcaacg cacgaattgc
     4741 atatctttgg aagtattaat ggagttgatt tcgacatggt ggggcagggt acgggaaacc
     4801 caaacgatgg ctatgaagaa cttaacctca agtctaccaa aggcgatctc caattctcac
     4861 cctggatatt ggtcccacac atcggctacg ggttccatca atacctgcct tatcctgacg
     4921 ggatgagtcc ttttcaagct gctatggttg acggaagtgg ctaccaagtt caccgcacaa
     4981 tgcaatttga ggatggtgca tctcttacgg ttaattaccg atatacatat gagggttcac
     5041 acatcaaggg ggaagctcag gtcaagggca ctggatttcc cgcagatggc ccagttatga
     5101 ccaattctct gaccgcagcc gattggtgtc gaagcaagaa gacctaccct aacgacaaga
     5161 ctatcatctc aaccttcaaa tggtcctaca ccacaggtaa cggaaagcgg tataggtcta
     5221 cagctcggac aacatatacc tttgcgaagc ctatggccgc taactatttg aaaaaccaac
     5281 cgatgtatgt ttttcggaag accgaattga aacattcaaa aacggagttg aatttcaaag
     5341 agtggcagaa ggcattcacc gacgtaatgg gtatggatga gttgtataaa taagcggcgg
     5401 ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt
     5461 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     5521 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     5581 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     5641 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     5701 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5761 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5821 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5881 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5941 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
     6001 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa
     6061 catttaacat ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa
     6121 tgctttatga ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa
     6181 tcatttcggc agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca
     6241 ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta
     6301 gacaccctta ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt
     6361 gtagggagtt gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca
     6421 agttagtcaa aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt
     6481 cgagatgcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6541 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6601 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6661 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6721 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt
     6781 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     6961 gatcttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     7021 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     7081 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     7141 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     7201 gggttaatct agtatacgcg tttaattaat ccagcttttg ttccctttag tgagggttaa
     7261 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7321 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7381 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7441 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8101 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9421 gaaaagtgcc ac
//
