LOCUS       sequence-330096-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding mouse Gnaq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, 
            Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M
  TITLE     Intravital imaging identifies the VEGF-TXA2 axis as a critical 
            promoter of PGE2 secretion from tumor cells and immune evasion.
  JOURNAL   Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 
            10.1158/0008-5472.CAN-20-4245.
  PUBMED    34035084
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 
            10.1158/0008-5472.CAN-20-4245."
COMMENT     Sequence Label: pPBbsr2-Gnaq
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..635
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(231..248)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     458..477
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(1106..1123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1112..1336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1383..1399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1407..1423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1431..1437,1438..1455,1456..1461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1476..1497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   1731..1793
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1834..1854)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1834..1852)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1873..1889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1897..1913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1921..1927,1928..1945,1946..1951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1966..1987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2104..2121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2275..2863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2355..2374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3034..3825,3826..3894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3657..3676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3895..3999)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4025..4480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4162..4183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4374..4393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4607..4629
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4621..4638
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4622..4638
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4648..4667
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4648..4666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4696..4730
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        5033..5412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5415..5690
                     /label=chicken beta-actin promoter
     intron          5691..6699
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6623..6645
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt tccctctagc
       61 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
      121 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
      181 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
      241 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
      301 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
      361 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
      421 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
      481 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
      541 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
      601 ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa catttaacat
      661 ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa tgctttatga
      721 ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc
      781 agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca ttgcgattgg
      841 tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta gacaccctta
      901 ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt
      961 gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca agttagtcaa
     1021 aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt cgagatgcat
     1081 cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1141 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1201 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1261 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1321 gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt ggcgtaatca
     1381 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1501 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatcttaaaa
     1561 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     1621 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     1681 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     1741 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct
     1801 agtatacgcg tttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     1861 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2701 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4021 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4081 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4141 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4201 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4261 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4321 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4381 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4441 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     4501 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     4561 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     4621 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggggcgc
     4681 gccattctag attaacccta gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa
     4741 atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg acatctcagt
     4801 cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa
     4861 ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac ttttaagatt
     4921 taactcatac gataattata ttgttatttc atgttctact tacgtgataa cttattatat
     4981 atatattttc ttgttataga tatcaactag aatgctagca tgggcccatc tcgacattga
     5041 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5101 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5161 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5221 tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5281 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5341 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5401 gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     5461 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     5521 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
     5581 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     5641 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     5701 cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac
     5761 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     5821 agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc
     5881 tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     5941 gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     6001 cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     6061 gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     6121 gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt
     6181 tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc
     6241 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     6301 cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg
     6361 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     6421 tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     6481 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     6541 gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt
     6601 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     6661 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     6721 gttgtgctgt ctcatcattt tggcaaagaa ttgggatccg aattcaccat gactctggag
     6781 tccatcatgg cgtgctgcct gagcgaggag gccaaggaag cccggaggat caacgacgag
     6841 atcgagcggc agctgcgcag ggacaagcgc gacgcccgcc gggagctcaa gctgctgctg
     6901 ctggggacag gggagagtgg caagagcacc ttcatcaagc agatgaggat catccacggg
     6961 tcgggctact ctgacgaaga caagcgcggc ttcaccaagc tggtgtatca gaacatcttc
     7021 acggccatgc aggccatgat cagagcgatg gacacgctca agatcccata caagtatgaa
     7081 cacaataagg ctcatgcaca attggttcga gaggttgatg tggagaaggt gtctgctttt
     7141 gagaatccat atgtagatgc aataaagagc ttgtggaatg atcctggaat ccaggagtgc
     7201 tacgacagac gacgggaata tcagttatct gactctacca aatactatct gaatgacttg
     7261 gaccgtgtag ccgacccttc ctatctgcct acacaacaag acgtgcttag agttcgagtc
     7321 cccactacag ggatcatcga ataccccttt gacttacaaa gtgtcatttt cagaatggtc
     7381 gatgtagggg gccaaaggtc agagagaaga aaatggatac actgctttga aaatgtcacc
     7441 tccatcatgt ttctagtagc gcttagcgaa tatgatcaag ttcttgtgga gtcagacaat
     7501 gagaaccgca tggaggagag caaagcactc tttagaacaa ttatcaccta cccctggttc
     7561 cagaacagta gcgtcatctt atttctgaat aagaaagatc ttctagagga gaaaatcatg
     7621 tattcccacc tagtcgacta cttcccagaa tatgatggac cccagagaga tgcccaggca
     7681 gctcgagaat tcatcctgaa aatgttcgtg gacctgaacc ccgacagtga caaaatcatc
     7741 tactcccact tcacgtgcgc cacagatacc gagaacatcc gcttcgtctt tgcagccgtc
     7801 aaggacacca tcctgcagct gaacctgaag gagtacaatc tggtctaa
//
