LOCUS       sequence-330112-        8637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1
            C-terminally tagged with 2x Strep-tag II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, 
            Rappsilber J, Skehel M, Carter AP
  TITLE     Shulin packages axonemal outer dynein arms for ciliary targeting.
  JOURNAL   Science. 2021 Feb 26;371(6532):910-916. doi: 
            10.1126/science.abe0526.
  PUBMED    33632841
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-26- 26"; volume: "371"; 
            issue: "6532"; pages: "910-916"
COMMENT     Sequence Label: pACEBac1-Q22MS1-2xStrep
FEATURES             Location/Qualifiers
     source          1..8637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5992..6015
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6016..6039
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6076..6099
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    6259..6393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6296..6315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6350..6369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6514..6679
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      6869..7457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7358..7377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  7592..7815
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7882..8415)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8604..8609,8610..8626,8627..8632))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcgatccct cgagcatgtt ccaatcttat ctccaggaca tcgagaagga gaacaactct
      361 agcatctaca acgcccaggc tcagcagaac tcaagcacac agcagtcaat ttgggatgac
      421 tacattgacc gccaactgga acacctcgta gtcgaatatc aattcgattt cgacaagatc
      481 gctcagagac tggctggagt cattggcgat aagcaacaac agaaggtgaa cttcgacacg
      541 tgtcgtcaaa gatggaagca gttgcatttg aggaggtatg aggacaagga caaggatagg
      601 ctcgaccgtg ttagcaaatc cgtcatgaag atggaagacc ctaacaacgc cctgaaggtg
      661 caagagctgg tcgagaagct gcccaaagcc cgtcagaata aggacttcct gaagattagc
      721 gacgaagaga agttgcaggc gttctacctg aacgacaagg gagagaagat ccaacctacc
      781 acctttaacg tgctcaaaga cagcgtaatg ggaatcaaag acaagacaga acgcgaaaga
      841 gtcttcaaga agcagcagga agagttcttc aagtctctgg agaagctggc aggcgagcaa
      901 gacgaggaca tgttcaccaa accactcatc ttcagagcca agtttacaga gcccaccaac
      961 tttaacttta tcgttgagag ggaagaattg acaagagagc aggctcaaag gattatgtct
     1021 cagaaggttc agatcaaagg tgcaggcttg gtgccaccag agcctgaaat gggcatcaag
     1081 atggacaatt acgtgtctat gtttgctaga cccgacgaag aagaggagga gttgaagaag
     1141 aaagaccagg agaaggaggt tcaggtcaac caacagatca tgcaggacca gcaggagact
     1201 aaggaggagc aagaacgcga tcaggaagaa ctgaagaaac agatggagct gacagaggag
     1261 gagaagaaga agcagcagga acaggaggag gagaagaaga agatcaatgt agacctctac
     1321 ttggctgagc gtggtctgaa atccattgac gaggccctca actcactgga gaagatgcgt
     1381 tcaaaggtta aagtgaacaa ctacttcgag gaggcacaaa ctgaagacaa gaagctgaag
     1441 gagcagaagg agaagaatca gcagaaagtt gacaatgagg atcaaattcg ccaagagatc
     1501 atcaagcagg gtctggagca actgaagcag aaggagcagg gagatcaact cgtccaattt
     1561 cacgagaatc agatcaagaa ggacatcaag gaactgaatg agattatcgg tgacaataag
     1621 agcaagaatg ttaagaacca aacgcagcaa cccaacaaag cagtagagga agaagaggaa
     1681 atcatcctgg agaagaagaa tgaccagagc aagaaagtac ccacaaagaa gggcatcttc
     1741 tctcaatttg cggaagaaga agactccgga tcagaggacg aagtaggaaa cgatgaggag
     1801 gagttggatg acgaactcta cctgcaagca caacgtattg gactcaagag cgatgcggag
     1861 atcgatctgt tcatcactaa gaagaaagaa gaacgcaagg ccaacatgct caacaagaag
     1921 aaagtacagt tctctgagtt ctcagagcag aagcaaatct ccgaagacac cgacgagctg
     1981 ctggctaagc aagagaaaca aatcaagcag aagcagcaat tcctggagaa gttgaagaag
     2041 aagagcgaga acgaagctgg cgagaacgac gagtcaaacc aggagggtag ccagaactgg
     2101 cgtgacctgg agttctccgc tggcatggct ttcaagcgcc aactggagga agagaagatg
     2161 cagaggatga tcaaggaaca gttcgaggag gaacaacgtc gtaaagccga ccaggagcgc
     2221 atccgccaga tccaagagga ataccacctg aagaaactcg acatcgaaac caagatcaag
     2281 ctcagagagc cactgaacct ggagaaggtg aaccaagaga agtggaaaga gtatatcgaa
     2341 gcacagcaga agagaagaga agagatcgag aaggaatttg aggagaagca acgtattcaa
     2401 actgagcaag aggagaaaga gcagaaagag aatctgatca aagagaagat ccacttcttt
     2461 gagcagaaga tcaagaactt tccctccaac gatcccttca agtgcttctt cgacgaccaa
     2521 gtatcttact tgtcccagat cagctccttg ccaagcaaga tcacgacgaa gatcgccgat
     2581 ctgaagaagt acgacatctc cagagatggt atcacgagca ggtatcaaat cctgatcttc
     2641 ccttacttgg acaaatgcca agagttcgta ttccaatttc tctccttctt cctccgtaac
     2701 tctttcttca gacaaccacc ctcagagaag gaactcggag aaattgtctc agagaatcct
     2761 ctggacgaaa ttccttgtca gaggctcagc agcaaaggct ttcccttctg tctggtgtca
     2821 ttctatatcg gctcacacac gagcgagatt aacgacgaac aggacgagca atacttgtcc
     2881 aattacctga atgacgcaat caagttcgat aaactcatca acgacgataa gctccagaag
     2941 agcatctacc tcttcttcga tacctacatt ggccgtggcc aagatactct gagcaagcag
     3001 ttggacttgg cactcacaga gttcctctca gatggttgct tggaccaagc tatccgtaag
     3061 tatggtgaga aggtcattaa cagggatatg aagctctcag acttcaagac agcttactat
     3121 aagaacatgg agatcgctaa gaagctgttc aacgagagca acaccttcca ttcaatctgt
     3181 cctaccaaga cctcatacca ccaaaccaat caactggcga ttcaccagaa taagaagaag
     3241 caactgctgc ccacatcacc atcaaatagc ccttcaatca tctctcactc acatggaaac
     3301 aagtgggaga catctgtcat tatcatccgt aactacagcg agattcagaa tccttcagct
     3361 ttgagacaac aacagaatcg tggcgtggac ttctctaacg cgagcgacct catgcagcag
     3421 gatgtgacca cggaacaata ctcaaacctg agcgagtctc tctcaaactt ggtgccttca
     3481 cataccgaaa tctcacagag gcagaaacag cagatgactg acgtgattca ctacacactg
     3541 aacaaactcc aagagaaggg tattcaaatc ttgggcatga agtccttctt cttcgatcag
     3601 aagacagtgc aagagttcaa atcttatgtg gagaacatta actttaacac gaacacaaat
     3661 cagcccatcg tcgtgatctc tgttgctggc ttcgatctcc actccaagct gaagaagatg
     3721 gtcggatctg aggacgcgca gctggcgaag cgcgtggagc caaattccct gaacgcgctc
     3781 ttctccacag gacgcgacga ctccatcatc attccctaca acaaggccct gtcccgtcgt
     3841 ctgatctcat acttcttctg tggacgcctg gagaagatcc agactgtaca ggacatccaa
     3901 gtgatcgagg gtctcaccaa caagaagcaa ctgtacctca ccaaacctaa accattcaat
     3961 cccgtattct tgttgaactc acaacccatc agagagcaca tttgcttgct ctcatccaag
     4021 ttcttgccac agcagttctg cgaagttatt cgcaagattc aagctcgcgg tttcgaactc
     4081 ctgaacatta gagtcatcga tagctccaac ctctccgcca tcctgaagaa agatgaggac
     4141 tttagcgcat tcattgagaa cgagaacttg caagaattga actctggtga gaacgactac
     4201 gagcgtatca acctcgttct gcatatccaa ggagccaata tcagctcact gcgcaacact
     4261 ctgctgaaca agattatcga ttacaatccc tttctccatc cctctaagag ggaaatcttc
     4321 tactacacca aggatgattt gtcattgacg ctgttgaagg acatcttctt caacaggcac
     4381 attaagtcca atttcacacc tagcgaacgt ttcccattgt ttatcgcact gtggccattc
     4441 ctcaatgact acgaggagaa agagggtctg acggcatccc aggctgtgcc tctcggcaac
     4501 gacttgaaga ttatgtcatc ctacggcctg gagctgctgt ctgtatccaa aatcttcaaa
     4561 tcccagatcc agcagaagct caacacttca atcgaagaag aaacagagca tgtggatttg
     4621 aaatccatcc tccaccagct gatccagaac aacattcaga tcaacagcat caccaagaag
     4681 aagcccgagt tctcaacctt ctgtagccgt ctgggtgaag agattctctc ccaagacttc
     4741 tatttggccg tattccgtta tcaaggcgat cgccagtcaa agcacctgga taagttgttt
     4801 acagaggtgc cattcgtcca ctccaacaag acgatcaacg aaagcacagt ggtaacgcat
     4861 ggcgctaaac aaacgcctca cacacaagca aagccactgc ccacggagtt cctcacgctc
     4921 ttctttctgg actcttctgc gcagcaagta tccaacttca ccaagctgtt gggcattgga
     4981 gccttcgacg attggtcttt caagggctcc tttatcttcg acctgccatc atctggatac
     5041 acaggcgaaa tcgagacact gatcattctg tcagaggacg tcgtcaagac cttgctgctg
     5101 gtgggattgg ttaagattat cgagaataac cagttcaaga tcaagagctt cttcaagcat
     5161 cgttgttcta gggagttctc agaggtcatt caagagaacc tgctccaggc gttctccggc
     5221 tacgaatccc aaatgcacga ccagggcacg aagttgcaga agattaaggc atctttggac
     5281 aacctcatga ctaggctcca gggagaatca atgtacttcc tggtactgtc taaggagaat
     5341 ggcatcttta acggtaagaa gatcctgttc gagttggcgg agaagtactc agtcttcaag
     5401 aaggaaacca tcaacgtaga atcttccacc gagtatatgc tggacaaagg catgtactgt
     5461 tctctgaatt acaaatgtca gcagcgcgag ttcaaagaac tccagtttat gtctcagcaa
     5521 attctgtcaa tggctcatga gaacaatcaa gaacagcagg acgtggacaa gatgtacgac
     5581 aatcaactgg tgatggttat ccgtatcgac cagcagatcc aactgtacga agtcttcagg
     5641 aaactccagt ctgacttgaa attcaagatc atccatttcc tggtcatgag gcaatttgag
     5701 tggactgaca ttcagtacat ctcttcacac tttacaccca agtttgaggc ccaacacatt
     5761 cagaaatact tcgagatcaa caacgatggt atctattgca ttgtccatct ggattccaag
     5821 aactcagtca gcctgtcaga tgcgtttggc accttgaggc tgaacgagaa gggcgagttc
     5881 aagcaggtga tccgttcctc attgttcgga aagcaggagg tgttcttcat ctacactaac
     5941 aatccacagc acgtgaagaa gctgtcagcc tacgccttca agtcttccga gctggaagtt
     6001 ctgtttcagg gtccgtggtc acatccgcag tttgaaaaag gtagcgcagg tagtgcagca
     6061 ggtagcggtg caggttggag ccatcctcag tttgagaaat aaggtacctc tagagcctgc
     6121 agtctcgaca agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
     6181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     6241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6361 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
     6421 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     6481 atcataaccc ctagggtata cccatctaat tggaaccaga taagtgaaat ctagttccaa
     6541 actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag ttcccatcta
     6601 ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc ccagttccca
     6661 actattttgt ccgcccacaa ccggttgact tgggtcaact gtcagaccaa gtttactcat
     6721 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6781 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7441 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7501 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttgacttggg tcgctcttcc
     7561 tgtggatgcg cagatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga
     7621 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
     7681 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
     7741 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat
     7801 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     7861 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     7921 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     7981 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     8041 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     8101 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     8161 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     8221 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     8281 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     8341 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     8401 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     8461 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     8521 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     8581 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactggg
//
