LOCUS       sequence-330115-        6273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3
            with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag 
            II at the C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, 
            Rappsilber J, Skehel M, Carter AP
  TITLE     Shulin packages axonemal outer dynein arms for ciliary targeting.
  JOURNAL   Science. 2021 Feb 26;371(6532):910-916. doi: 
            10.1126/science.abe0526.
  PUBMED    33632841
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-26- 26"; volume: "371"; 
            issue: "6532"; pages: "910-916"
COMMENT     Sequence Label: pACEBac1-Q22YU3 del C3-2xStrep
FEATURES             Location/Qualifiers
     source          1..6273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3628..3651
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3652..3675
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3712..3735
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    3895..4029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3932..3951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3986..4005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4150..4315
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4505..5093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4994..5013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5228..5451
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5518..6051)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6240..6245,6246..6262,6263..6268))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcgatccct cgagcatgtt taacttcttc tcctctgcta acatcaatca gaacatccca
      361 aagtattccg taaatgactt cgtgttccgc ctgaagaaga tcgagaagat cgtggtcaag
      421 gaaggattgg acggtttctt gctgatcaat ggcgtagatt cacgcgagaa cacagagtac
      481 gttaaactca cgaattggct gttcctgggt aactctggcc tggagatcga ggagaatgag
      541 tacctgaacc aaatctacag cgacatgatt gtgttgatta agaagggaac cacccacatc
      601 ttcattgacc ctgaggctct caattccttg caaaccctga tctactcaat tcccaacgtg
      661 gatgtcttct gtcccaccga gaagcaatac gaggacaagg atgaaatgga actcttgaag
      721 atggccttct tcctgagagt aatgaagcca accaagaagg tgggcatcct gttgggccag
      781 aaggataagg gtaagatcaa cagcatcgag aagtggcctt tgattcaaag ctacggtctg
      841 gaagaactcg gtgtgggctt cttctcaatg aatcatgagg ttgtggatct gacactgcgt
      901 ctgaacgcgg tgtacaagaa ctatgacaaa ttctttgttt ctaaactcat ctacgtagtt
      961 gccaagagac tgactggtca ctttaactca gccgctggtc aactcggtga catgaagatg
     1021 cataaacgca acttggccac ggaatcacaa ctcacggaaa tctttcgtga cacttatgag
     1081 attgaggaga ttagcaaatg ggtccaaatt cgcggtgtga atgcagcgtt gcccaaaccc
     1141 agagtgttgt tcggcaagaa cacgtcagct gattgctcaa aggagccctc tgtagcacca
     1201 ctgaaggatc tcaagtattc tgaaacgttc cactctttcc acgctacatt cgagacattt
     1261 gatctcagga catgtctcag ggcagctcgc acctacttcc tggctaaagg tgttaaagag
     1321 gagagaaact tgatcacact gaatgacgac gagggagtcc ctcaaggtta tgagttgaac
     1381 attgatgaga accaacagta caaggatcag gacttcctcg ctaacttgta cttgagcatc
     1441 atcattggct ttaacgaggt tatgcagttg attaccaaag actacaagaa catgaccgag
     1501 gagttcatcc aggattacat cttccagaag gtctccaaag tgtatgctgg cttccaaatc
     1561 cctgaatcag aaatcacatt ggacaagatc caaatcattc tgaaagcgta caattcattc
     1621 ggtgaagagg tgaagattga cttcaaagac actatctcat tcaagttgac accatacttc
     1681 ttcatggttc gtatcgaaca gaagaacatc aagagccaaa tcctgaacaa tacggtattg
     1741 ggttctctcg tgttcgctga gtctttcatt ctccaggagg gatgctacct gctgctcact
     1801 aaagagatcc catacttcga tctgtggaac tgtcagaacg actactcaga gaagatcgag
     1861 aagatgaaga agcgtatctt gtgggaacca ctgggcaaac aaatctccga cgaactgccc
     1921 aagaacagga tctttgtcca gactggcaga aagagcaatt acggctttga cattcccatt
     1981 atgcaggctt cctattacat gcacgaactc ggtctgcgca tcgaaactca acgcctgggc
     2041 tggttcatct tgttcttcaa ggagatgaag gagatccaaa tcacacagaa gatgaatcac
     2101 acctggctga tcttcaaagt ggattccaac atcaccttta actcaattag caaggacaca
     2161 atcgcgttgg agttcactgg agatgccttg gagcagagct tctttaagat caagaattac
     2221 ttcgaggaga atcaaatcaa atacgagtac caagtagaca tcccagcaat cttccaggag
     2281 tctcagatcg ccaagaaaca aatcctcaat cagcaatcac aaggtcagaa actcattacc
     2341 atgaactcaa tccagaatga acagttcttc atctcttaca ttgaaagcaa acagctcatg
     2401 attctcaacc agatgaagga cttgaaattg tccgcctaca agaacctgta cgaacaaatg
     2461 cagatttctc aggctatcac tcccgttgag aaccacatcg gtgtcatcct ggttaacggt
     2521 agctactgct ccggtaagcg taagttcgct gagaacctca tccgcttcgg cagcgacaac
     2581 aatctccgcc tgcacctgta caaattcgat ctgaacgaga tgtcagaact cacagagaag
     2641 tcctacttga gcggactgct caaattcgcg agcgagaaga agatccagaa cacggacgtt
     2701 attgtggctt ccgtccctca ctttatcaac acgaagattt tgatcgacta tttcagcaag
     2761 tcagagaaga tttcaaatgc cttctacatt cgcacaatcg cgaccaagat caacatcaac
     2821 aacatctaca gcaactttaa caagaatccc gtcaacaacg tgttcactta tggtgtggaa
     2881 ggctactccc aattcctcct cctggatacc tacaacaact acgatgcaga cgtgaacgct
     2941 ctgaacaaga cgctctccgg agtcctccct ggtgccaaaa tttacaagat tatgaacaat
     3001 atcctgaatc ccgcattggc aaaggatatt ctgacgtcca ttaccttcat cagcgaacag
     3061 aacaatttga accgtctgaa gtactcagtg caatacgacc tgctcacttc caatggtcct
     3121 tcttcagtcg tgttcattcc ctttaagttg ccaattctga gggagaagat ccgtgacctc
     3181 atctataaga agattttgca gaacggaaac cagactttgg tggacacgat tgaagccgag
     3241 cagaagatcg ccgagttcaa ggagctgaat aagaactcta aagacccttt gatgatcgag
     3301 atcattaagc tgaaggagaa gatcgagatc cagaacgcac agacttccga ccaagccatc
     3361 aagatcgatt atgttaaagg tatcctgcgc tacgactcca agctgaagga aggtctcgaa
     3421 gagattacta tcacaccaaa ctacttcatt gaacgcactg tgaagggtgt tgacgcaaag
     3481 gagtttactg aagaactcaa cggagtctca ttcaagaatg tgaagtacac aggaatcact
     3541 aattccatca ttaacgatat gggattcgtg ttcgctggca agaacctgaa caaagagaag
     3601 ttgctggagc tgctgtacaa attggtcctg gaagttctgt ttcagggtcc gtggtcacat
     3661 ccgcagtttg aaaaaggtag cgcaggtagt gcagcaggta gcggtgcagg ttggagccat
     3721 cctcagtttg agaaataagg tacctctaga gcctgcagtc tcgacaagct tgtcgagaag
     3781 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3841 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4021 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     4081 agctgagttg gctgctgcca ccgctgagca ataactatca taacccctag ggtataccca
     4141 tctaattgga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
     4201 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
     4261 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacaaccgg
     4321 ttgacttggg tcaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4621 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5161 ctgcgttatc ccctgattga cttgggtcgc tcttcctgtg gatgcgcaga tgccctgcgt
     5221 aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca
     5281 ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa
     5341 aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt
     5401 gcccgtcgta ttaaagaggg gcgtggccaa gggcatgtaa agactatatt cgcggcgttg
     5461 tgacaattta ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta
     5521 ggtggcggta cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt
     5581 atagagagcc actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc
     5641 aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg
     5701 gtgctcgccg gagactgcga gatcatagat atagatctca ctacgcggct gctcaaactt
     5761 gggcagaacg taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc
     5821 gatgaatgtc ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga
     5881 gtaggtggct acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt
     5941 gacttggtca gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt
     6001 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc
     6061 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     6121 tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac
     6181 cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca
     6241 gtttacgaac cgaacaggct tatgtcaact ggg
//
