LOCUS       sequence-33015-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, 
            Roberts AG, Oparka KJ, Christie JM
  TITLE     The photoreversible fluorescent protein iLOV outperforms GFP as a 
            reporter of plant virus infection.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Dec 16. 105(50):20038-43.
  PUBMED    19060199
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Dec 16. 105(50):20038-43."
COMMENT     Sequence Label: pUC57 iLOV CHLAMY
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..360
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MIEKNFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNARFLQ
                     GPETDQATVQKIRDAIRDQRETTVQLINYTKSGKKFWNLLHLQPVRDQKGELQYFIGVQ
                     LDG"
     primer_bind     complement(447..463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(460..482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    471..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(495..501,502..519,520..525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgcgaatgc atctagatcc aatgacaccc aatgcaaaca tgatcgagaa gaacttcgtg
       61 atcaccgacc cccgcctgcc cgacaacccc atcatcttcg ccagcgacgg cttcctggag
      121 ctgaccgagt acagccgcga ggagatcctg ggccgcaacg cccgcttcct gcagggcccc
      181 gagaccgacc aggccaccgt gcagaagatc cgcgacgcca tccgcgacca gcgcgagacc
      241 accgtgcagc tgatcaacta caccaagagc ggcaagaagt tctggaacct gctgcacctg
      301 cagcccgtgc gcgaccagaa gggcgagctg cagtacttca tcggcgtgca gctggacggc
      361 agcgaccacg tgtaaccaat gaccccaata tcggatcccg ggcccgtcga ctgcagaggc
      421 ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      901 cagaagtggc gaaacccgac aggactataa agataccagg cgttttcccc ctggaagctc
      961 ccttcgtggt tctgt
//
