LOCUS       sequence-330161-        4509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure 
            realtime changes in FRET reflecting fluctuations in cAMP levels in 
            living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   van der Krogt GN, Ogink J, Ponsioen B, Jalink K
  TITLE     A comparison of donor-acceptor pairs for genetically encoded FRET 
            sensors: application to the Epac cAMP sensor as an example.
  JOURNAL   PLoS ONE. 2008  . 3(4):e1916.
  PUBMED    18382687
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(4):e1916."
COMMENT     Sequence Label: Epac-S-H96
FEATURES             Location/Qualifiers
     source          1..4509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..729)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2965..3699
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(3757..3759,3760..3762,3763..4473)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atggcggccg caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      721 ctgtacaagt ccggactcag atctcgatat cagcccgtgg gaactcatga gatggaggag
      781 gagttggccg aagctgtggc cctgctctcc cagcgggggc ctgacgccct gctcactgtg
      841 gcacttcgaa agcccccagg tcagcgcacg gatgaagagc tggacctcat ctttgaggag
      901 ctgctgcaca tcaaggctgt ggcccacctc tccaactcgg tgaagcgaga attagcggct
      961 gttctgctct ttgaaccaca cagcaaggca gggaccgtgt tgttcagcca gggggacaag
     1021 ggcacttcgt ggtacattat ctggaaggga tctgtcaacg tggtgaccca tggcaagggg
     1081 ctggtgacca ccctgcatga gggagatgat tttggacagc tggctctggt gaatgatgca
     1141 ccccgggcag ccaccatcat cctgcgagaa gacaactgtc atttcctgcg tgtggacaag
     1201 caggacttca accgtatcat caaggatgtg gaggcaaaga ccatgcggct ggaagaacat
     1261 ggcaaagtgg tgctggtgct ggagagagcc tctcagggcg ccggcccttc ccgaccccca
     1321 accccaggca ggaaccggta tacagtgatg tctggcaccc cagagaagat cctagagctt
     1381 ctgttggagg ccatgggacc agattccagt gctcatgacc caacagagac attcctcagc
     1441 gacttcctcc tgacccacag ggtcttcatg cccagcgccc aactctgcgc tgcccttctg
     1501 caccacttcc atgtggagcc tgcgggtggc agcgagcagg agcgcagcac ctacgtctgc
     1561 aacaagaggc agcagatctt gcggctggtc agccagtggg tggccctgta tggctccatg
     1621 ctccacactg accctgtggc caccagcttc ctccagaaac tctcagacct ggtgggcagg
     1681 gacacccgac tcagcaacct gctgagggag cagtggccag agaggcggcg atgccacagg
     1741 ttggagaatg gctgtgggaa tgcatctcct cagatgaagg cccggaactt gcctgtttgg
     1801 ctccccaacc aggacgagcc ccttcctggc agcagctgtg ccatccaagt tggggataaa
     1861 gtcccctatg acatctgccg gccagaccac tcagtgttga ccctgcagct gcctgtgaca
     1921 gcctccgtga gagaggtgat ggcagcgttg gcccaggagg atggctggac caaggggcag
     1981 gtgctggtga aggtcaattc tgcaggtgat gccattggcc tgcagccaga tgcccgtggt
     2041 gtggccacat ctctggggct caatgagcgt ctctttgttg tcaacccaca ggaagcgcat
     2101 gagctgatcc cacaccctga ccagctgggg cccactgtgg gctctgctga ggggctggac
     2161 ctggtgagtg ccaaggacct ggcaggccag ctgacggacc acgactggag cctcttcaac
     2221 agtatccacc aggtggagct gatccactat gtgctgggcc cccagcatct gcgggatgtc
     2281 accaccgcca acctggagcg cttcatgcgc cgcttcaatg agctgcagta ctgggtggcc
     2341 accgagctgt gtctctgccc cgtgcccggc ccccgggccc agctgctcag gaagttcatt
     2401 aagctggcgg cccacctcaa ggagcagaag aatctcaatt ccttctttgc cgtcatgttt
     2461 ggcctcagca actcggccat cagccgccta gcccacacct gggagcggct gccccacaaa
     2521 gtccggaagc tgtactccgc cctcgagagg ctgctggatc cctcatggaa ccaccgggta
     2581 taccgactgg ccctcgccaa gctctcccct cctgtcatcc ccttcatgcc ccttcttctc
     2641 aaagacatgg ccgccattca tgagggaaac cacacactag tggagaatct catcaacttt
     2701 gagaagatga gaatgatggc cagagccgcg cggatgctgc accactgccg aagccacaac
     2761 cctgtgcctc tctcaccact cagaagccga gtttcccacc tccacgagga cagccaggtg
     2821 gcgaggattt ccacatgctc ggagcagtcc ctgagcaccc ggagtccagc cagcacctgg
     2881 gcttatgtcc agcagctgaa ggtcattgac aaccagcggg aactctcccg cctctcccga
     2941 gagctggagc cagctagcga gctcatggac ggcggcgtgc agctcgccga ccactaccag
     3001 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     3061 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3121 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg cagcggtggc
     3181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3241 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3301 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3361 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     3421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3481 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3601 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     3661 ggcatcaagg ccaacttcaa gatccgccac aacatcgagc cgaattttgt cttcctgatc
     3721 ggcgccgcag gaatactctt cgtatctagc gagctcatgg tgagcaaggg cgaggagctg
     3781 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3841 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     3901 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccactct gggctacggc
     3961 ctacagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4021 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4081 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4141 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4201 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     4261 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     4321 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     4381 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4441 gggatcactc tcggcatgga cgagctgtac aagccgaatt ccctcgaggt taacgcggcg
     4501 cgcctctaa
//
