LOCUS       sequence-330163-        4491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure 
            realtime changes in FRET reflecting fluctuations in cAMP levels in 
            living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4491)
  TITLE     Jalink Lab Biosensor Plasmids
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Epac-S-H127
FEATURES             Location/Qualifiers
     source          1..4491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(43..45,46..48,49..561)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2947..3681
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(3739..3741,3742..3744,3745..4455)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtgcggccg caatggtgag caagggcgag
       61 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      121 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc
      241 tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
      481 tttagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
      541 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
      601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      661 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      721 gccgcccgat atcagcccgt gggaactcat gagatggagg aggagttggc cgaagctgtg
      781 gccctgctct cccagcgggg gcctgacgcc ctgctcactg tggcacttcg aaagccccca
      841 ggtcagcgca cggatgaaga gctggacctc atctttgagg agctgctgca catcaaggct
      901 gtggcccacc tctccaactc ggtgaagcga gaattagcgg ctgttctgct ctttgaacca
      961 cacagcaagg cagggaccgt gttgttcagc cagggggaca agggcacttc gtggtacatt
     1021 atctggaagg gatctgtcaa cgtggtgacc catggcaagg ggctggtgac caccctgcat
     1081 gagggagatg attttggaca gctggctctg gtgaatgatg caccccgggc agccaccatc
     1141 atcctgcgag aagacaactg tcatttcctg cgtgtggaca agcaggactt caaccgtatc
     1201 atcaaggatg tggaggcaaa gaccatgcgg ctggaagaac atggcaaagt ggtgctggtg
     1261 ctggagagag cctctcaggg cgccggccct tcccgacccc caaccccagg caggaaccgg
     1321 tatacagtga tgtctggcac cccagagaag atcctagagc ttctgttgga ggccatggga
     1381 ccagattcca gtgctcatga cccaacagag acattcctca gcgacttcct cctgacccac
     1441 agggtcttca tgcccagcgc ccaactctgc gctgcccttc tgcaccactt ccatgtggag
     1501 cctgcgggtg gcagcgagca ggagcgcagc acctacgtct gcaacaagag gcagcagatc
     1561 ttgcggctgg tcagccagtg ggtggccctg tatggctcca tgctccacac tgaccctgtg
     1621 gccaccagct tcctccagaa actctcagac ctggtgggca gggacacccg actcagcaac
     1681 ctgctgaggg agcagtggcc agagaggcgg cgatgccaca ggttggagaa tggctgtggg
     1741 aatgcatctc ctcagatgaa ggcccggaac ttgcctgttt ggctccccaa ccaggacgag
     1801 ccccttcctg gcagcagctg tgccatccaa gttggggata aagtccccta tgacatctgc
     1861 cggccagacc actcagtgtt gaccctgcag ctgcctgtga cagcctccgt gagagaggtg
     1921 atggcagcgt tggcccagga ggatggctgg accaaggggc aggtgctggt gaaggtcaat
     1981 tctgcaggtg atgccattgg cctgcagcca gatgcccgtg gtgtggccac atctctgggg
     2041 ctcaatgagc gtctctttgt tgtcaaccca caggaagcgc atgagctgat cccacaccct
     2101 gaccagctgg ggcccactgt gggctctgct gaggggctgg acctggtgag tgccaaggac
     2161 ctggcaggcc agctgacgga ccacgactgg agcctcttca acagtatcca ccaggtggag
     2221 ctgatccact atgtgctggg cccccagcat ctgcgggatg tcaccaccgc caacctggag
     2281 cgcttcatgc gccgcttcaa tgagctgcag tactgggtgg ccaccgagct gtgtctctgc
     2341 cccgtgcccg gcccccgggc ccagctgctc aggaagttca ttaagctggc ggcccacctc
     2401 aaggagcaga agaatctcaa ttccttcttt gccgtcatgt ttggcctcag caactcggcc
     2461 atcagccgcc tagcccacac ctgggagcgg ctgccccaca aagtccggaa gctgtactcc
     2521 gccctcgaga ggctgctgga tccctcatgg aaccaccggg tataccgact ggccctcgcc
     2581 aagctctccc ctcctgtcat ccccttcatg ccccttcttc tcaaagacat ggccgccatt
     2641 catgagggaa accacacact agtggagaat ctcatcaact ttgagaagat gagaatgatg
     2701 gccagagccg cgcggatgct gcaccactgc cgaagccaca accctgtgcc tctctcacca
     2761 ctcagaagcc gagtttccca cctccacgag gacagccagg tggcgaggat ttccacatgc
     2821 tcggagcagt ccctgagcac ccggagtcca gccagcacct gggcttatgt ccagcagctg
     2881 aaggtcattg acaaccagcg ggaactctcc cgcctctccc gagagctgga gccagctagc
     2941 gagctcatgg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3001 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     3061 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3121 actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag caagggcgag
     3181 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3241 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3301 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
     3361 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     3421 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3481 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3541 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3601 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     3661 aagatccgcc acaacatcga gccgaatttt gtcttcctga tcggcgccgc aggaatactc
     3721 ttcgtatcta gcgagctcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3781 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3841 gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg
     3901 cccgtgccct ggcccaccct cgtgaccact ctgggctacg gcctacagtg cttcgcccgc
     3961 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4021 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4081 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4141 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
     4201 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     4261 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4321 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     4381 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4441 gacgagctgt acaagccgaa ttccctcgag gttaacgcgg cgcgcctcta a
//
