LOCUS       sequence-33017-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Trichas G, Begbie J, Srinivas S
  TITLE     Use of the viral 2A peptide for bicistronic expression in transgenic
            mice.
  JOURNAL   BMC Biol. 2008 Sep 15;6:40. doi: 10.1186/1741-7007-6-40.
  PUBMED    18793381
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-09-15"; volume: "6:40. doi"; pages: " 
            10.1186/1741-7007-6-40"
COMMENT     Sequence Label: pCAG-TAG
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..461)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(480..533)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(626..649)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtggcccgcg gtagcgctag cccacctccg ccgctgcccc cacctccaga tccacccccg
       61 ccggagcctc cacccccaga tcccttagcg ctggtgtact tggtgacggc cttagtaccc
      121 tcggacacgg cgtgcttggc caactcccca ggcagcagca ggcgcacggc cgtctggatc
      181 tccctggagg tgatggtcga gcgcttgttg taatgcgcca ggcgggaagc ctcacctgcg
      241 atgcgctcga aaatgtcgtt cacaaacgaa ttcatgatgc ccatggcctt ggacgaaatg
      301 ccggtgtcag ggtggacctg cttcagaacc ttgtacacat agatggaata gctctccttg
      361 cggctgcgct tgcgcttctt gccgcctttc ttctgcgcct tagtcaccgc cttcttggag
      421 ccctttttcg gggcgggagc agacttcgct ggctctggca tggtgatatt cagatctcct
      481 gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc ctctccactg
      541 cccttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc ggagcgctcg
      601 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg
      661 tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt gaactccacc
      721 aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt cagcacgccg
      781 tcgcgggggt acaggcgctc ggtggaaggc t
//
