LOCUS       sequence-330177-        4479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure 
            realtime changes in FRET reflecting fluctuations in cAMP levels in 
            living cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Klarenbeek J, Goedhart J, van Batenburg A, Groenewald D, Jalink K
  TITLE     Fourth-generation epac-based FRET sensors for cAMP feature 
            exceptional brightness, photostability and dynamic range: 
            characterization of dedicated sensors for FLIM, for ratiometry and 
            with high affinity.
  JOURNAL   PLoS One. 2015 Apr 14;10(4):e0122513. doi: 
            10.1371/journal.pone.0122513. eCollection 2015.
  PUBMED    25875503
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-14"; volume: "10(4):e0122513. doi"; pages: " 
            10.1371/journal.pone.0122513. eCollection 2015"
COMMENT     Sequence Label: Epac-S-H189
FEATURES             Location/Qualifiers
     source          1..4479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             2917..3651
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             3709..4443
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 atggcggccg caatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac tttagcgaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgcccgat atcagcccgt gggaactcat
      721 gagatggagg aggagttggc cgaagctgtg gccctgctct cccagcgggg gcctgacgcc
      781 ctgctcactg tggcacttcg aaagccccca ggtcagcgca cggatgaaga gctggacctc
      841 atctttgagg agctgctgca catcaaggct gtggcccacc tctccaactc ggtgaagcga
      901 gaattagcgg ctgttctgct ctttgaacca cacagcaagg cagggaccgt gttgttcagc
      961 cagggggaca agggcacttc gtggtacatt atctggaagg gatctgtcaa cgtggtgacc
     1021 catggcaagg ggctggtcac caccctgcat gagggagatg attttggaga gctggctctg
     1081 gtgaatgatg caccccgggc agccaccatc atcctgcgag aagacaactg tcatttcctg
     1141 cgtgtggaca agcaggactt caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg
     1201 ctggaagaac atggcaaagt ggtgctggtg ctggagagag cctctcaggg cgccggccct
     1261 tcccgacccc caaccccagg caggaaccgg tatacagtga tgtctggcac cccagagaag
     1321 atcctagagc ttctgttgga ggccatggga ccagattcca gtgctcatga cccaacagag
     1381 acattcctca gcgacttcct cctgacccac agggtcttca tgcccagcgc ccaactctgc
     1441 gctgcccttc tgcaccactt ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc
     1501 acctacgtct gcaacaagag gcagcagatc ttgcggctgg tcagccagtg ggtggccctg
     1561 tatggctcca tgctccacac tgaccctgtg gccaccagct tcctccagaa actctcagac
     1621 ctggtgggca gggacacccg actcagcaac ctgctgaggg agcagtggcc agagaggcgg
     1681 cgatgccaca ggttggagaa tggctgtggg aatgcatctc ctcagatgaa ggcccggaac
     1741 ttgcctgttt ggctccccaa ccaggacgag ccccttcctg gcagcagctg tgccatccaa
     1801 gttggggata aagtccccta tgacatctgc cggccagacc actcagtgtt gaccctgcag
     1861 ctgcctgtga cagcctccgt gagagaggtg atggcagcgt tggcccagga ggatggctgg
     1921 accaaggggc aggtgctggt gaaggtcaat tctgcaggtg atgccattgg cctgcagcca
     1981 gatgcccgtg gtgtggccac atctctgggg ctcaatgagc gtctctttgt tgtcaaccca
     2041 caggaagcgc atgagctgat cccacaccct gaccagctgg ggcccactgt gggctctgct
     2101 gaggggctgg acctggtgag tgccaaggac ctggcaggcc agctgacgga ccacgactgg
     2161 agcctcttca acagtatcca ccaggtggag ctgatccact atgtgctggg cccccagcat
     2221 ctgcgggatg tcaccaccgc caacctggag cgcttcatgc gccgcttcaa tgagctgcag
     2281 tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc
     2341 aggaagttca ttaagctggc ggcccacctc aaggagcaga agaatctcaa ttccttcttt
     2401 gccgtcatgt ttggcctcag caactcggcc atcagccgcc tagcccacac ctgggagcgg
     2461 ctgccccaca aagtccggaa gctgtactcc gccctcgaga ggctgctgga tccctcatgg
     2521 aaccaccggg tataccgact ggccctcgcc aagctctccc ctcctgtcat ccccttcatg
     2581 ccccttcttc tcaaagacat ggccgccatt catgagggaa accacacact agtggagaat
     2641 ctcatcaact ttgagaagat gagaatgatg gccagagccg cgcggatgct gcaccactgc
     2701 cgaagccaca accctgtgcc tctctcacca ctcagaagcc gagtttccca cctccacgag
     2761 gacagccagg tggcgaggat ttccacatgc tcggagcagt ccctgagcac ccggagtcca
     2821 gccagcacct gggcttatgt ccagcagctg aaggtcattg acaaccagcg ggaactctcc
     2881 cgcctctccc gagagctgga gccagctagc gagctcatgg acggcggcgt gcagctcgcc
     2941 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3001 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3061 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     3121 ggcagcggtg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3181 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3241 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     3301 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     3361 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3421 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3481 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3541 atcctggggc acaagctgga gtacaactgg aacagccaca acgtctatat caccgccgac
     3601 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga gccgaatttt
     3661 gtcttcctga tcggcgccgc aggaatactc ttcgtatcta gcgagctcat ggacggcggc
     3721 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     3781 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     3841 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     3901 ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagctgtt caccggggtg
     3961 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4021 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     4081 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     4141 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4201 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4261 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4321 gaggacggca acatcctggg gcacaagctg gagtacaact ggaacagcca caacgtctat
     4381 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     4441 gagccgaatt ccctcgaggt taacgcggcg cgcctctaa
//
