LOCUS       sequence-33018-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Trichas G, Begbie J, Srinivas S
  TITLE     Use of the viral 2A peptide for bicistronic expression in transgenic
            mice.
  JOURNAL   BMC Biol. 2008 Sep 15;6:40. doi: 10.1186/1741-7007-6-40.
  PUBMED    18793381
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-09-15"; volume: "6:40. doi"; pages: " 
            10.1186/1741-7007-6-40"
COMMENT     Sequence Label: pCS2-TAG
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..419)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(438..491)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(584..607)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tctccagatc cacccccgcc ggagcctcca cccccagatc ccttagcgct ggtgtacttg
       61 gtgacggcct tagtaccctc ggacacggcg tgcttggcca actccccagg cagcagcagg
      121 cgcacggccg tctggatctc cctggaggtg atggtcgagc gcttgttgta atgcgccagg
      181 cgggaagcct cacctgcgat gcgctcgaaa atgtcgttca caaacgaatt catgatgccc
      241 atggccttgg acgaaatgcc ggtgtcaggg tggacctgct tcagaacctt gtacacatag
      301 atggaatagc tctccttgcg gctgcgcttg cgcttcttgc cgcctttctt ctgcgcctta
      361 gtcaccgcct tcttggagcc ctttttcggg gcgggagcag acttcgctgg ctctggcatg
      421 gtgatattca gatctcctgg gccaggattc tcctcgacgt caccgcatgt tagcagactt
      481 cctctgccct ctccactgcc cttgtacagc tcgtccatgc cgtacaggaa caggtggtgg
      541 cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      601 tccagcttgg tgtccacgta gtagtagccg ggcagttgca cgggcttctt ggccatgtag
      661 atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc ctg
//
