LOCUS       sequence-330186-        1290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   van der Wal J, Habets R, Varnai P, Balla T, Jalink K
  TITLE     Monitoring agonist-induced phospholipase C activation in live cells 
            by fluorescence resonance energy transfer.
  JOURNAL   J Biol Chem. 2001 May 4. 276(18):15337-44.
  PUBMED    11152673
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 May 4. 276(18):15337-44."
COMMENT     Sequence Label: PIP2-CFP(ST) sensor
FEATURES             Location/Qualifiers
     source          1..1290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggactcagat ctcgagctca agcttcgaat tcccgggtct cagccatgga ctcgggccgg
      781 gacttcctga ccctgcacgg cctacaggat gatgaggatc tacaggcgct gctgaagggc
      841 agccagctcc tgaaggtgaa gtccagctca tggaggagag agcggttcta caagttgcag
      901 gaggactgca agaccatctg gcaggagtcc cgcaaggtca tgcggacccc ggagtcccag
      961 ctgttctcca tcgaggacat tcaggaggtg cgaatggggc accgcacgga gggtctggag
     1021 aagttcgccc gtgatgtgcc cgaggaccgc tgcttctcca ttgtcttcaa ggaccagcgc
     1081 aatacactag acctcatcgc cccatcgcca gctgatgccc agcactgggt gctggggctg
     1141 cacaagatca tccaccactc aggctccatg gaccagcgtc agaagctaca gcactggatt
     1201 cactcctgct tgcgaaaagc tgacaaaaac aaggacaaca agatgagctt caaggagctg
     1261 cagaacttcc tgaaggagct caacatctga
//
