LOCUS       sequence-33019-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Trichas G, Begbie J, Srinivas S
  TITLE     Use of the viral 2A peptide for bicistronic expression in transgenic
            mice.
  JOURNAL   BMC Biol. 2008 Sep 15;6:40. doi: 10.1186/1741-7007-6-40.
  PUBMED    18793381
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-09-15"; volume: "6:40. doi"; pages: " 
            10.1186/1741-7007-6-40"
COMMENT     Sequence Label: pCS2-Tm2AG
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..415)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     primer_bind     complement(580..603)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cagatccacc cccgccggag cctccacccc cagatccctt agcgctggtg tacttggtga
       61 cggccttagt accctcggac acggcgtgct tggccaactc cccaggcagc agcaggcgca
      121 cggccgtctg gatctccctg gaggtgatgg tcgagcgctt gttgtaatgc gccaggcggg
      181 aagcctcacc tgcgatgcgc tcgaaaatgt cgttcacaaa cgaattcatg atgcccatgg
      241 ccttggacga aatgccggtg tcagggtgga cctgcttcag aaccttgtac acatagatgg
      301 aatagctctc cttgcggctg cgcttgcgct tcttgccgcc tttcttctgc gccttagtca
      361 ccgccttctt ggagcccttt ttcggggcgg gagcagactt cgctggctct ggcatggtga
      421 tattcagatc tcctggggca gcattctcct cgacgtcacc gcatgttagc agacttcctc
      481 tgccctctcc actgcccttg tacagctcgt ctatgccgta caggaacagg tggtggcggc
      541 cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      601 gcttggtgtc cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg
      661 tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc
      721 ccttcagcac gccgtcgcgg gggtacaggc gctc
//
