LOCUS       sequence-330192-        1263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PH-YFP(UST) fusion used to measure realtime changes in PIP2 levels. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1263)
  TITLE     Jalink Lab Biosensor Plasmids
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIP2-YFP(UST) sensor
FEATURES             Location/Qualifiers
     source          1..1263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccaa actgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagccg
      721 aattcccggg tctcagccat ggactcgggc cgggacttcc tgaccctgca cggcctacag
      781 gatgatgagg atctacaggc gctgctgaag ggcagccagc tcctgaaggt gaagtccagc
      841 tcatggagga gagagcgctt ctacaagttg caggaggact gcaagaccat ctggcaggag
      901 tcccgcaagg tcatgcggac cccggagtcc cagctgttct ccatcgagga cattcaggag
      961 gtgcgaatgg ggcaccgcac ggagggtctg gagaagttcg cccgtgatgt gcccgaggac
     1021 cgctgcttct ccattgtctt caaggaccag cgcaatacac tagacctcat cgccccatcg
     1081 ccagctgatg cccagcactg ggtgctgggg ctgcacaaga tcatccacca ctcaggctcc
     1141 atggaccagc gtcagaagct acagcactgg attcactcct gcttgcgaaa agctgacaaa
     1201 aacaaggaca acaagatgag cttcaaggag ctgcagaact tcctgaagga gctcaacatc
     1261 tga
//
