LOCUS       sequence-330193-        1662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear 
            export signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MCP-ADAR2-DD(E488Q, N496F)
FEATURES             Location/Qualifiers
     source          1..1662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgctgccac cattggaaag gcttacgctg gggagcgact acaaagacga tgacgacaag
       61 gggagcggat ccggctctat ggcgtccaat ttcactcagt ttgtgctggt tgacaacggc
      121 gggaccgggg acgttacggt agccccctca aactttgcca acggtatagc ggagtggata
      181 agcagcaatt ctaggagtca agcatacaaa gttacatgca gcgtgcgcca atctagcgct
      241 cagaatcgca agtacaccat taaagtagag gtccccaagg gagcctggag aagctatctt
      301 aacatggagt tgaccatacc aatcttcgct accaactctg actgtgaact cattgtgaaa
      361 gccatgcaag gtctgctcaa ggatggtaac ccaattccgt ccgctatcgc tgccaactct
      421 gggatttacg ggggcagtgg gagcggtgca ggatctggta gtccagctgg gggaggagca
      481 ccgggtagcg gtggggggtc tcagctgcac ctgccccagg ttctcgcaga cgccgtatcc
      541 cgccttgtac tgggcaagtt tggtgatctt actgacaatt tttcatctcc tcatgcgagg
      601 cggaaagtac tcgcaggcgt cgtcatgacg accggaactg acgtgaaaga cgccaaagtc
      661 atctctgtct ccacgggcac aaagtgcata aacggggagt acatgagcga ccgggggctg
      721 gcactgaatg attgtcacgc tgaaataata tctaggcgat ctctgcttag atttctctac
      781 actcaactcg aattgtacct taacaacaaa gatgaccaga aacgcagtat atttcagaaa
      841 tcagaacgcg gcggatttcg acttaaggaa aacgttcagt tccacttgta tatcagcaca
      901 tccccttgcg gtgacgcccg aatcttttcc ccgcacgagc cgatattgga ggagcccgcg
      961 gacagacatc ctaataggaa ggctagaggc caacttcgga cgaagattga aagtggcgag
     1021 ggtactatcc cggtgcggtc cttcgctagt attcaaacgt gggacggagt ccttcaaggt
     1081 gaacggctgt tgacaatgag ctgctcagac aaaatcgcgc gctggaatgt agtgggaatc
     1141 caaggcagcc tcttgagcat attcgtagaa cccatatatt tctcatccat tattttgggc
     1201 tctctgtatc atggtgacca tctgtcaagg gctatgtacc aacgaatttc taatatcgag
     1261 gatcttcctc cactctatac actcaataag cctctcttgt ccgggatatc aaacgctgag
     1321 gcccgccagc cagggaaagc tcctaacttc agtgttaact ggaccgttgg tgattctgcg
     1381 atagaggtca tcaacgccac gacaggtaag gatgagctcg gtagagcctc acgcctgtgt
     1441 aaacacgcgt tgtattgtag atggatgaga gtacatggga aggtcccatc tcacttgctc
     1501 cgaagcaaga tcactaagcc taatgtgtat catgagtcaa aactcgcggc taaagaatac
     1561 caggcagcca aagctcgact ttttacagct tttattaagg cagggctcgg ggcatgggtc
     1621 gagaagccga ccgagcagga ccaattctct ctgacgccct ga
//
