LOCUS       sequence-33020-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Smith SP, Barber KR, Dunn SD, Shaw GS
  TITLE     Structural influence of cation binding to recombinant human brain 
            S100b: evidence for calcium-induced exposure of a hydrophobic 
            surface.
  JOURNAL   Biochemistry. 1996 Jul 9. 35(27):8805-14.
  PUBMED    8688416
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 1996 Jul 9. 35(27):8805-14."
COMMENT     Sequence Label: pSS2 (hS100B)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(639..661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    650..666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(674..680,681..696,697..702))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aacgccagct agcttgacct taatttaagc gtcaggtgga tgtttttgct cttatttcga
       61 tcaatgaata aacaggaaaa caggggggtt ttatatcagc aggatctatg tgaacgctat
      121 tcaggacggg tcacacgcgc aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt
      181 ttcaagccgg tgttacatcg cttttttggt ccaagcttcc taattagcta caacacggct
      241 ggaaagctca gctcctacta ggctgcaagc ccttgctgtc tgctttcttg catgaccatc
      301 tctgttacag gaaaggtttg gctgctttct aatctcactc atgttcaaag aactcgtggc
      361 aggcagtagt aaccatggca acaaaggcca tgaattcctg gaagtcacat tcgccgtctc
      421 catcattgtc cagtgtttcc atgactttgt ccacaacctc ctgctctttg atttcctcta
      481 agaaatggga aagctcattg ttgatgagct ccttcagttc tgacttcttc agcttgtgct
      541 tgtcaccctc tctccctgaa tactgatgga agacatcaat gagggcaacc atggccttct
      601 caagctcaga cattttaaaa tcctccagaa ttctgtttcc tgtgtgaaat tgttatccgc
      661 tcacaattcc acacattata cgagccgatg attaattgtc aacagctcat ttcagaatat
      721 ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg
      781 agcgacacga aattatgcag tgatttacga cctgcacagc cataccacag cttccgatgg
      841 ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat aagcccggat
//
