LOCUS       sequence-330210-       10053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: APT3-PTEN (PTEN high)
FEATURES             Location/Qualifiers
     source          1..10053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1355..1807
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4673..5653)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5717..6796)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6796..8070)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     promoter        complement(8251..8269)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      8833..9055
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9103..10053
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaaaatggct cgtgtacagt ttaaacaacg
     1381 tgaatctact gacgcaatct ttgttcactg ctcggctacc aagccaagtc agaatgttgg
     1441 tgtccgtgag attcgccagt ggcacaaaga gcagggttgg ctcgatgtgg gataccactt
     1501 tatcatcaag cgagacggta ctgtggaggc aggacgagat gagatggctg taggctctca
     1561 cgctaagggt tacaaccaca actctatcgg cgtctgcctt gttggtggta tcgacgataa
     1621 aggtaagttc gacgctaact ttacgccagc ccaaatgcaa tcccttcgct cactgcttgt
     1681 cacactgctg gctaagtacg aaggcgctgt gcttcgcgcc catcatgagg tggcgccgaa
     1741 ggcttcccct tcgttcgacc ttaagcgttg gtgggagaag aacgaactgg tcacatctga
     1801 ccgtggtagc ggcggtggtg cgagtggtgg tgcggagaac ggttccttat gcgaccaaga
     1861 gatcgattcg atctgcagta tcgagagagc agataacggc ggtagcgcag gcagtggagc
     1921 gggcggtaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
     1981 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
     2041 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact
     2101 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
     2161 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc tcggcaggcg
     2221 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
     2281 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
     2341 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc
     2401 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
     2461 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
     2521 ggcgtggtct tcgtggtata agaaagactc tattcatgta ggagtacgct gcatcgagat
     2581 gctcattaag tcaactggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca
     2641 agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg
     2701 tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac
     2761 tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac
     2821 tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc
     2881 gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa
     2941 cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga
     3001 actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg
     3061 tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagacttga
     3121 gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa
     3181 catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat
     3241 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
     3301 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
     3361 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
     3421 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
     3481 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
     3541 ttgctctgtc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
     3601 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
     3661 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
     3721 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
     3781 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
     3841 cgggtccaaa gagttcggct tccgtcaaca agtgctggac gataccattc agccagctat
     3901 tgattccggc aagggtctga tgttcaccca gccgaatcag gctgctagat acatggctaa
     3961 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
     4021 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
     4081 tcgcaagcgt tgcgctgtgc attggataac tcctgatggt ttccctgtgt ggcaggaata
     4141 caagaagcct attcagacgc gcttggacct gatgttcctc ggtcagttcc gcttacagct
     4201 taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagg ctggtatcgc
     4261 tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca
     4321 cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc
     4381 tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg
     4441 tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga
     4501 caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga
     4561 cttcgcgttc gcgtaatgga gattttcaac atgacgccat gggcattaaa aaatgccctc
     4621 ttgggttatc aagagggtca ttatatttgc tgaggagtgc cgttaattaa gtttaggtat
     4681 attccgtgtg gtacttctta atattatcat caacaatatt gattacattt ttttggctca
     4741 tcaaatcatt cattggttca aaggacagca atacactttt cgcaccacac ttttcaattg
     4801 ccaacttagc cgcagttata cactccgtat aatttccgac agcgttttct gcaattattt
     4861 cttcaagttt attttcgaaa ttttcattag ggtgcatttc aagaacataa tcactaataa
     4921 atgcacgcgt ctcttgttta gctttattac tatcttcgtt atagttaact aatatcatta
     4981 actgatggtc tatctctgat aggtcaacgt catatttatc cgcaacggct ttatatcttt
     5041 cagcatattc atatctaaca tcattagaat catcccactt aaagatgaga ggaatacctt
     5101 ttttggccgc ccactcaaca atatgatgac tggttgctgt tacatatttc cgaggtccgc
     5161 ctggcgtata agcatgggga tttacagata ttttagggaa gctataaaaa tcgttatctg
     5221 gattacaata gcctgttgtt aaagcatcgt taatgatttc ataacactct tcaaatagtt
     5281 gctgttgata ttcaaccggg cgattaaaaa aatgcatttc atcttttttt tcgcaatcac
     5341 taaaccctaa aataaatctc ccttcactta actgatccaa taagcaagct tcctccgcta
     5401 tgcggacagg atgatgagtt gtaatgatgt gatttaatga accaatttta attttctctg
     5461 ttaaaccgag cagaaaacca gaaacagtca gaggagcgcc gacaacacca ttatctgaaa
     5521 aatgattttc atacactaaa atctgttcaa aattcaactt atcaacatac tccgttattt
     5581 cctgcatgcg aactatactc tgttcttgaa cagttgttga attgatgaag ttaaggaaga
     5641 acaatccaaa tttgctaccg ccaccgccgc taccgccacc gccgctaccg ccaccgccgc
     5701 taccgccacc gccataatat aatagcgaac gttgtttttc tttaagaaat ggcatgacat
     5761 cagactggaa gagcttcatg gaagcaataa tttcgtctac tgttccatta gcttcaaatc
     5821 cacaacaaat atttgatatt cctgtagcat caatgtcttt ttgaattatg tcaatacatt
     5881 cctgcggcgt tcccacggga ttgatttcgt aactgtaatc aatacggcga ttagtatctt
     5941 tatgtccttt taatacaaag tcacgccact gccctttatt gaaatcataa cctcttgttt
     6001 ggtctgaatc atcaaaaata gtcgtagcat tcacataaga atcataccaa tgccccagaa
     6061 atttccggca aatctctttc gctttaattg agtcatgatc tacagatgtt atatatgata
     6121 agcaatggtc gatattatga atatcgtgcc catattcttg agccacttca ttataaagct
     6181 caagttgtgc tttcttttcg ttagtattta taatccaact taatatcatc ggtaggccaa
     6241 attgagcagc ccactcagtc gtcgaagctg attcagccac cacataaacc ggtgcgccac
     6301 ctctgctata cgccgcgggg tttactttta ccttatggaa cttgatatgt tcattatcag
     6361 cttccatata tccctctgtc atgccattct ttatcagccc gtaccagcat tccgctaagg
     6421 cgcgactgtt attcatatct gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc
     6481 aaataccaaa ccgaaatcgt ccttttgaca tttgatccaa taaattcaca tcttcaagtt
     6541 ggcgtactgg atgggctgtg ggaagaacaa tagcggcagt tcctacattc aattttttag
     6601 tcgcgccaag taaatatgca gcagcgacat aagggttacc aagcaaacca aactccgtga
     6661 aatgatgctc cagtaaccat acggtatcaa aaccacactc ctcagagatg cgacctaatt
     6721 taaccaaacg tttcattacc tctgtttggg aaaattgggg aggttggtat gtaagcaaaa
     6781 agtttccaaa tttcattaag actccttatt acgcagtatg ttagcaaacg tagaaaatac
     6841 atacataaag gtggcaacat ataaaagaaa cgcaaagaca ccgcggaaca ggttgatctt
     6901 atcgcagtcg atactgaact cgtaaggttt accagcgcca aagacaaaag ggcgacattc
     6961 aaccgattga gggagggaag gtaaatattg acggaaatta ttcattaaag gtgaattatc
     7021 accgtcaccg acttgagcca tttgggaatt agagccagca aaatcaccag tagcaccatt
     7081 accattagca aggccggaaa cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt
     7141 agcgacagaa tcaagtttgc ctttagcgtc agactgtagc gcgttttcat cggcattttc
     7201 ggtcatagcc cccttattag cgtttgccat cttttcataa tcaaaatcac cggaaccaga
     7261 gccaccaccg gaaccgcctc cctcagagcc gccaccctca gaaccgccac cctcagagcc
     7321 accaccctca gagccgccac cagaaccacc accagagccg ccgccagcat tgacaggagg
     7381 ttgaggcagg tcagacgatt ggccttgata ttcacaaacg aatggatcct cattaaagcc
     7441 agaatggaaa gcgcagtctc tgaatttacc gttccagtaa gcgtcataca tggcttttga
     7501 tgatacagga gtgtactggt aataagtttt aacggggtca gtgccttgag taacagtgcc
     7561 cgtataaaca gttaatgccc cctgcctatt tcggaaccta ttattctgaa acatgaaagt
     7621 attaagaggc tgagactcct caagagaagg attaggatta gcggggtttt gctcagtacc
     7681 aggcggataa gtgccgtcga gagggttgat ataagtatag cccggaatag gtgtatcacc
     7741 gtactcagga ggtttagtac cgccaccctc agaaccgcca ccctcagaac cgccaccctc
     7801 agagccacca ccctcatttt cagggatagc aagcccaata ggaacccatg taccgtaaca
     7861 ctgagtttcg tcaccagtac aaactacaac gcctgtagca ttccacagac agccctcata
     7921 gttagcgtaa cgatctaaag ttttgtcgtc tttccagacg ttagtaaatg aattttctgt
     7981 atggggtttt gctaaacaac tttcaacagt ttcagcggag tgagaataga aaggaacaac
     8041 taaaggaatt gcgaataata attttttcat tttttccttt ttactgcacc tgcaggtctt
     8101 tcttatgagt tacctcctga acttggtaaa cagaacaaca aagctgcctg atagtggcct
     8161 ttatttttgg cataaataac agaataaaca ctgcactgtg tattcattcc aacgagtgaa
     8221 tacacggagc aatgtcgctc cttctttctc cctttagtga gggttaattg tagggtccct
     8281 aagtctcctc agcaaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgac
     8341 cggatgtcct cttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg
     8401 tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat
     8461 cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca
     8521 taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat
     8581 gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg
     8641 cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa
     8701 ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa
     8761 ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact
     8821 gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt tttttattct
     8881 ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta
     8941 gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat
     9001 ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc
     9061 aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt
     9121 ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta
     9181 attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg
     9241 acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct
     9301 tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat
     9361 cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa
     9421 aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc
     9481 ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat
     9541 gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc
     9601 cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt
     9661 aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg
     9721 gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga actagataga
     9781 caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata
     9841 ccaacaacca ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat
     9901 gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa
     9961 agtaagcatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta
    10021 gagaacatac tggctaaata cggaaggatc tga
//
