LOCUS       sequence-330218-        8361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for Candida albicans containing a red-shifted 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Dorsaz S, Coste AT, Sanglard D
  TITLE     Red-Shifted Firefly Luciferase Optimized for Candida albicans In 
            vivo Bioluminescence Imaging.
  JOURNAL   Front Microbiol. 2017 Aug 3;8:1478. doi: 10.3389/fmicb.2017.01478. 
            eCollection 2017.
  PUBMED    28824601
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2017-08-3"; volume: "8:1478. doi"; pages: " 
            10.3389/fmicb.2017.01478. eCollection 2017"
COMMENT     Sequence Label: pDS1928
FEATURES             Location/Qualifiers
     source          1..8361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2759..2778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2760..2778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2785..2802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2785..2801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2794..2816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2942..3397)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3079..3100)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3291..3310
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3424..3528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3529..3597,3598..4389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3747..3766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4560..5148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5049..5068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5302..5319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5436..5457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5472..5477,5478..5495,5496..5502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5510..5526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5515..5537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5534..5550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5534..5550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5571..5589
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgcgtagatc caactcaagt acaacatggc tgtcggtaaa aacaagagat tgtccaaagg
       61 aaagaaagga ttaaaaaaga aggtcgttga cccattcacc agaaaagatt ggtttgacat
      121 caaagctcca accacttttg aaaacagaaa tgttggtaaa actttgatca acagatctac
      181 cggtttaaag aatgccgctg atggcttgaa aggtagagtt ttcgaagttt gtttggccga
      241 cttacaaggt tccgaagacc actcttacag aaaaatcaaa ttgagagttg atgaagtcca
      301 aggtaaaaac ttgttgacca acttccatga ggcctccatg gaggcctcat ggaggcctca
      361 tggaggcctc atggaggcct catggtttgg atttcacttc tgacaaatta agatcattgg
      421 tcagaaaatg gcaatcatta gttgaagcta atgtcactgt taaaacttcc gacgattacg
      481 ttttgagagt ttttgccatt gctttcacca aaagacaacc aaaccaaatc aagaaaacta
      541 cttacgctca atcctctaaa ttgagagaag tcagaaagaa gatgattgaa attatgcaaa
      601 gagaagtttc caactgtact ttagctcaat taacttccaa attgattcca gaagtcattg
      661 gccgtgaaat tgaaaaatcc acccaaacca ttttcccatt acaaaatgtc cacatcagaa
      721 aagtcaaatt gttgaaacaa ccaaaattcg acttgggttc attattggct ttgcacggtg
      781 aaggttcaac cgaagaaaaa ggtaagaaag tttcttctgg tttcaaagat gttgttttag
      841 aatctgttta aggataagga tctgggggat ccggaccacc tttgattgta aatagtaata
      901 attaccaccc ttatctaatt atttatttaa cttatttatt tatttattat acatatatac
      961 aaatctaata aagtgaaaat ctcccccttc acacttcaca tatgttaggc gtcatcctgt
     1021 gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta
     1081 tacgtgaaga ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca
     1141 ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac
     1201 tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca
     1261 acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca
     1321 agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag
     1381 atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat
     1441 aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc
     1501 acctgctcag ggatcaccga aattttcata tgagaaccgt tatcgataac taaagcagca
     1561 acttcttcta taaaaatggg ttagtatgac agtcatttaa ataaggaatt tttcagttgg
     1621 cttggtttca attcaatgtt cgtttttttt tttcttgctg tgtttgtgtt tgtgttgtct
     1681 atagttgtgt gcactgagcg tcgaaaaaaa aaattcatag tgagccggga aatctgtata
     1741 gcccagataa caacacaagt ccaaactaga aactcgtcaa acaccaaaag caatgttgaa
     1801 tcaattgcct tgcacaagta cacgtaggaa aacataaaac attgcaattt tgaatattga
     1861 gccttttgtc gtaacattga ttgataggat tactcaccga atggttttga aaccactgcc
     1921 gacagatcaa tcaatcaatc aaaaaacgtg aactttgaaa aaggggaaga acagatacat
     1981 tgaagttagc catttccact gatcgtcaca acatatctga taaattactt tcaaaattat
     2041 aagctgatgt gtgtgtatta ttaatgtgac agtaacatcc caaacgagaa atattatgtc
     2101 gacaacaaaa aagtttgatc tgaattgaaa atgaagtttt cccaccctac ccatttgtca
     2161 tattgaaacc aatcaactga ttaatcaatc aattagaatt gaagctaaac taaaacatac
     2221 caccgtccat tttgaatgat tatatttttt taatattaat atcgagataa tgtttctaag
     2281 aaagaaagaa aaccaggagt gaaaattaga aaaggaaagg aaaggaaaaa aagaaaaatc
     2341 tgaaaatata taaaaaaaaa ttgtttcgtt ggcaataaat cttggtgaga acagcgaccg
     2401 aaagcaaata agaacaaaat atgagtgtat tacgttgaac aactaattaa cgtgtgtgta
     2461 tggatctttt tttctttttt ctctttaacc gactataaac aacaaacatt tttgggcagt
     2521 gcacacacta cttaatatac acagcataaa ttacacgatt agaaacaaat tagcttatta
     2581 aaataaccta atcaaaccga atattttatg gtattatgag taaactatat aatataaata
     2641 gcacacaccc acaacaacaa caaaggaaaa ctaaaaggtt ttttcttttt gaaaagatcg
     2701 ttttctttat tattctctag ttttgacgcg gccgccaccg cggtggagct ccaattcgcc
     2761 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2821 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2881 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2941 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3001 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3061 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3121 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3181 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3241 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3301 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3361 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     3421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4681 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5341 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5401 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5461 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5521 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct
     5581 cactaaaggg aacaaaagct ggtaccgggc cccccctcga gctattaaga tcaccagcct
     5641 cgtttataat aaacttagtc taatatttct tttggaccaa aatagaaact gttctcaggt
     5701 ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc ggctatgcca atcaaaaagg
     5761 agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg cgaaacaaca attttaatgc
     5821 ttactgtgcc gccaccaata tcctatggcc aaggggcctt taaacatgcc acggaaatat
     5881 acagtcaaag taaacctact atgtctttag agccttcagg atcaattgtt tttaaatgaa
     5941 tgactgcaaa actagaaaag tagcctctta cataagcaag tcatttgtac accgagagtt
     6001 tagtaaccag tgaagcaagt atatctggca aacctgaaac ttatctgtga attataaagc
     6061 tcataagtaa aaagtgtaga aaagaagata aggcagacca aatacaaatt tgcaacagta
     6121 caacgaatat ttcagcgtca aaactagaga ataataaaga aaacgatctt ttcaaaaaga
     6181 aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg tgctatttat attatatagt
     6241 ttactcataa taccataaaa tattcggttt gattaggtta ttttaataag ctaatttgtt
     6301 tctaatcgtg taatttatgc tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt
     6361 gttgtttata gtcggttaaa gagaaaaaag aaaaaaagat ccatacacac acgttaatta
     6421 gttgttcaac gtaatacact catattttgt tcttatttgc tttcggtcgc tgttctcacc
     6481 aagatttatt gccaacgaaa caattttttt ttatatattt tcagattttt ctttttttcc
     6541 tttcctttcc ttttctaatt ttcactcctg gttttctttc tttcttagaa acattatctc
     6601 gatattaata ttaaaaaaat ataatcattc aaaaagctta aaatggaaga tgctaagaac
     6661 atcaagaaag gtccagctcc attctaccca ttggaagatg gtactgctgg tgaacaattg
     6721 cacaaagcta tgaaaagata cgctttggtt ccaggtacta ttgctttcac tgatgctcac
     6781 attgaagttg atatcactta cgctgaatac tttgaaatgt ctgtcagatt ggctgaagct
     6841 atgaaaagat acggtttgaa caccaaccac agaattgttg tttgtagtga aaactctttg
     6901 caattcttca tgccagtttt gggtgctttg ttcattggtg ttgctgttgc tccagctaac
     6961 gatatttaca acgaaagaga attgttgaac tccatgggta tttctcaacc aactgttgtt
     7021 ttcgtttcta agaaaggttt gcaaaagatc ttgaacgttc aaaagaaatt gccaattatt
     7081 caaaagatca tcatcatgga ttctaaaact gattaccaag gtttccaatc tatgtacact
     7141 ttcgttactt ctcacttgcc accaggtttc aacgaatacg atttcgttcc agaatctttt
     7201 gacagagaca aaaccattgc tttgattatg aactcttctg gttctactgg tttgccaaaa
     7261 ggtgttgctt tgccacacag agctttgtgt gttagattct ctcacgctag agatccaatt
     7321 ttcggtaacc aaatcattcc agatactgct attttgtctg ttgttccatt ccaccacggt
     7381 ttcggtatgt tcactacttt gggttacttg atttgtggtt tcagagttgt tttgatgtac
     7441 agattcgaag aagaattgtt cttgagatct ttgcaagatt acaagattca aactgctttg
     7501 ttggttccaa ctttgttctc tttcttggct aaatctactt tgattgataa atacgatttg
     7561 tctaacttgc acgaaattgc ttctggtggt gctccattgt ctaaagaagt tggtgaagct
     7621 gttgctaaaa gattccactt gccaggtatt agacaaggtt acggtttgac tgaaaccact
     7681 tctgctattt tgatcactcc agaaggtgat gacaaaccag gtgctgttgg taaagttgtt
     7741 ccattctttg aagctaaagt tgttgatttg gatactggta aaactttggg tgttaaccaa
     7801 agaggtgaat tgtgtgttag aggtccaatg atcatgtctg gttacgttaa caacccagaa
     7861 gctaccaacg ctttgattga taaagatggt tggttacact ctggtgatat tgcttactgg
     7921 gatgaagatg aacacttctt cattgttgac agattgaaat ctttgatcaa atacaaaggt
     7981 taccaagttg ctccagctga attggaatct atcttgttgc aacacccaaa catttttgat
     8041 gctggtgttg ctggtttgcc agatgatgat gctggtgaat tgccagctgc tgttgttgtt
     8101 ttggaacacg gtaaaaccat gactgaaaaa gaaattgttg attacgttgc ttctcaagtt
     8161 accactgcta agaaattgag aggtggtgtt gttttcgttg atgaagttcc aaaaggtttg
     8221 actggtaaat tggatgctag aaagatcaga gaaattttga tcaaagctaa gaaaggtggt
     8281 aaaattgctg tataggctag cgtccctatt tattttttta tagttatgtt agtattaaga
     8341 acgttattta tatttcaaat t
//
