LOCUS       sequence-330219-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 
            variant for C-U editing) with a nuclear export signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: Split-RESCUE-N
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..30
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgctgccac cattggaaag gcttacgctg gggagcgact acaaagacga tgacgacaag
       61 gggagcggat ccggctctat ggcgtccaat ttcactcagt ttgtgctggt tgacaacggc
      121 gggaccgggg acgttacggt agccccctca aactttgcca acggtatagc ggagtggata
      181 agcagcaatt ctaggagtca agcatacaaa gttacatgca gcgtgcgcca atctagcgct
      241 cagaatcgca agtacaccat taaagtagag gtccccaagg gagcctggag aagctatctt
      301 aacatggagt tgaccatacc aatcttcgct accaactctg actgtgaact cattgtgaaa
      361 gccatgcaag gtctgctcaa ggatggtaac ccaattccgt ccgctatcgc tgccaactct
      421 gggatttacg ggggcagtgg gagcggtgca ggatctggta gtccagctgg gggaggagca
      481 ccgggtagcg gtggggggag ccagctgcac ctgccccagg ttctcgcaga cgccgtatcc
      541 cgccttgtaa tcggcaagtt tggtgatctt actgacaatt tttcatctcc tcatgcgagg
      601 cggatcggcc tcgcaggcgt cgtcatgacg accggaactg acgtgaaaga cgccaaagtc
      661 atctgcgtct ccacgggcag caagtgcata aacggggagt acctgagcga ccgggggctg
      721 gcactgaatg attgtcacgc tgaaatagtg tctaggcgat ctctgcttag atttctctac
      781 actcaactcg aattgtacct taacaacgag gatgaccaga aacgcagtat atttcagaaa
      841 tcagaacgcg gcggatttcg acttaaggaa aacatccagt tccacttgta tatcagcaca
      901 tccccttgcg gtgacgcccg aatcttttcc ccgcacgagg ccatattgga ggagcccgcc
      961 tag
//
