LOCUS       sequence-330220-        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector carrying the RESCUE-DDC-lambda-N (RESCUE is the evolved 
            ADAR2 variant for C-U editing) with a nuclear export signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: Split-RESCUE-C
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1165..1191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1198..1224
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 gacagacatc ctaataggaa ggctagaggc caacttcgga cgaagattga agccggccag
       61 ggtactatcc cggtgcggaa caacgctagt attcaaacgt gggacggagt ccttcaaggt
      121 gaacggctgt tgacaatgag ctgctcagac aaaatcgcgc gctggaatgt agtgggaatc
      181 caaggcagcc tcttgagcat attcgtagaa cccatatatt tctcatccat tattttgggc
      241 tctctgtatc atggtgacca tctgtcaagg gctatgtacc aacgaatttc taatatcgag
      301 gatcttcctc cactctatac actcaataag cctctcttga ccgggatatc aaacgctgag
      361 gcccgccagc cagggaaagc tcctatcttc agtgttaact ggaccgttgg tgattctgcg
      421 atagaggtca tcaacgccac gacaggtaag ggcgagctcg gtagagcctc acgcctgtgt
      481 aaacacgcgt tgtattgtag atggatgaga gtacatggga aggtcccatc tcacttgctc
      541 cgaagcaaga tcactaagcc taatgtgtat catgagacca aactcgcggc taaagaatac
      601 caggcagcca aagctcgact ttttacagct tttattaagg cagggctcgg ggcatgggtc
      661 gagaagccga ccgagcagga ccaattctct ctgacgcccg ggggcagtgg gagcggtgca
      721 ggatctggta gtccagctgg gggaggagca ccgggtagcg gtggggggag caatgcgagg
      781 acacggcgac gagaacgacg cgctgaaaag caagcgcaat ggaaagctgc aaacggaggt
      841 ggaggatctg gcggtggagg gtcaggtggc ggcggttcaa atgctcggac tcggagacgc
      901 gagcggagag cggagaagca ggcacaatgg aaagccgcta atggtggcgg cgggagtggg
      961 ggaggcggat ctggaggtgg cgggtccaac gcgagaaccc gccgacgaga gcgcagagcg
     1021 gagaaacaag cacagtggaa ggcggccaac gggggtgggg gatctggcgg cgggggatcc
     1081 ggcggaggag ggtccaatgc ccgaacccga cgaagggaaa gacgggctga gaaacaggct
     1141 caatggaaag cggctaatgg gagctaccca tacgatgttc cagattacgc tggatccctg
     1201 ccaccattgg aaaggcttac gctgtga
//
