LOCUS       sequence-330232-        8853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_FLAG.Ollas.VSVg_NGFR
FEATURES             Location/Qualifiers
     source          1..8853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     813..833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1016..1039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1058..1099
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1121..1153
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     misc_feature    1916..2504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1969..1989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2387..2398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2579..2812
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2884..3005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2921..2940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2975..2994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3045..3180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3200..3219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3201..3219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3229..3246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3229..3245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3238..3260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3387..3842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3474..3493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3684..3705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3868..3972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3973..4041,4042..4833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4191..4210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5004..5592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5493..5512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5746..5763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5880..5901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5916..5921,5922..5939,5940..5946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5954..5970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5959..5981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5978..5994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5978..5994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6013..6033
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6015..6033
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6061..6287
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6288..6468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6515..6640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7133..7366
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7551..7595
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7893..8141
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7893..7913
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8064..8083
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8160..8235
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    8283..8400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(8540..8853,1..865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(8771..8853,1..856)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
       61 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
      181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
      241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
      301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
      361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
      421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
      481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
      541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
      601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
      661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
      721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
      781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
      841 ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc
      901 gggatccacg gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg
      961 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcgacta
     1021 caaagacgat gacgacaagg gaggaggcgg gggagggagc ggcttcgcca acgagctggg
     1081 ccccaggctg atgggcaagt gcggaggagg cgggggaggg tatacagata tcgaaatgaa
     1141 tagactcgga aagcccacag gcctgtacac acacagcggt gagtgctgca aagcctgcaa
     1201 cctgggcgag ggtgtggccc agccttgtgg agccaaccag accgtgtgtg agccctgcct
     1261 ggacagcgtg acgttctccg acgtggtgag cgcgaccgag ccgtgcaagc cgtgcaccga
     1321 gtgcgtgggg ctccagagca tgtcggcgcc gtgcgtggag gccgacgacg ccgtgtgccg
     1381 ctgcgcctac ggctactacc aggatgagac gactgggcgc tgcgaggcgt gccgcgtgtg
     1441 cgaggcgggc tcgggcctcg tgttctcctg ccaggacaag cagaacaccg tgtgcgagga
     1501 gtgccccgac ggcacgtatt ccgacgaggc caaccacgtg gacccgtgcc tgccctgcac
     1561 cgtgtgcgag gacaccgagc gccagctccg cgagtgcaca cgctgggccg acgccgagtg
     1621 cgaggagatc cctggccgtt ggattacacg gtccacaccc ccagagggct cggacagcac
     1681 agcccccagc acccaggagc ctgaggcacc tccagaacaa gacctcatag ccagcacggt
     1741 ggcaggtgtg gtgaccacag tgatgggcag ctcccagccc gtggtgaccc gaggcaccac
     1801 cgacaacctc atccctgtct attgctccat cctggctgct gtggttgtgg gccttgtggc
     1861 ctacatagcc ttcaagaggt ggaacagggg tatcctctag agcggccgcg tcgacaatca
     1921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     1981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2281 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     2341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc atcttcgcct
     2461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgggt acctttaaga
     2521 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2581 gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac tgggtctctc
     2641 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2701 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2761 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     2821 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     2881 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2941 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3001 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     3061 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3121 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3181 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3241 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3301 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3361 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     3421 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3481 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3541 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3601 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3661 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3721 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3781 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     3841 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3901 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3961 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4021 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4081 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4141 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4201 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4261 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4321 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4381 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4441 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4501 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4561 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4621 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4681 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4741 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4801 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4861 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4921 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4981 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5041 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5101 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5161 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5221 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5281 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5341 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5401 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5461 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5521 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5581 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5641 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5701 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5761 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5821 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     5881 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     5941 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6001 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     6061 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     6121 ccttacaagg agagaaaaag caccgtgcat ggcgattggt ggaagtaagg tggtacgatc
     6181 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     6241 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     6301 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6361 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6421 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6481 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6541 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6601 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6661 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6721 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6781 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6841 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6901 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6961 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7021 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7081 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7141 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7201 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7261 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7321 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7381 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7441 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7501 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7561 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7621 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7681 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7741 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     7801 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     7861 gagactagta tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     7921 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     7981 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     8041 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     8101 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggtcttcg agaagacctg
     8161 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     8221 gcaccgagtc ggtgcttttt ttaattctcg acctcgagac aaatggcagt attcatccac
     8281 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     8341 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     8401 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggc gataagcttt
     8461 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     8521 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     8581 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     8641 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     8701 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     8761 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     8821 cccttgcgtg ccttgaatta cttccacctg gct
//
