LOCUS       sequence-33024-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: GFPZFN2-B2H
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccaccat ggactacaaa gacgatgacg acccaaaaaa gaagcgaaag gtacccttcc
      181 agtgtcgcat ttgcatgcgg aacttttcgg cgccgagcaa gcttgacagg catacccgta
      241 ctcatactgg tgaaaaaccg tttcagtgtc ggatctgtat gcgaaatttc tccgatcgct
      301 ctaatctgac ccgccatcta cgtacgcaca ccggcgagaa gccattccaa tgccgaatat
      361 gcatgcgcaa cttcagtgag ggggggaacc tgatgaggca cctaaaaacc cacacaggac
      421 agaaggacca actagtcaaa agtgaactgg aggagaagaa atctgaactt cgtcataaat
      481 tgaaatatgt gcctcatgaa tatattgaat taattgaaat tgccagaaat tccactcagg
      541 atagaattct tgaaatgaag gtaatggaat tttttatgaa agtttatgga tatagaggta
      601 aacatttggg tggatcaagg aaaccggacg gagcaattta tactgtcgga tctcctattg
      661 attacggtgt gatcgtggat actaaagctt atagcggagg ttataatctg ccaattggcc
      721 aagcagatga aatgcaacga tatgtcgaag aaaatcaaac acgaaacaaa catatcaacc
      781 ctaatgaatg gtggaaagtc tatccatctt ctgtaacgga atttaagttt ttatttgtga
      841 gtggtcactt taaaggaaac tacaaagctc agcttacacg attaaatcat atcactaatt
      901 gtaatggagc tgttcttagt gtagaagagc ttttaattgg tggagaaatg attaaagccg
      961 gcacattaac cttagaggaa gtgagacgga aatttaat
//
