LOCUS       sequence-330242-        7261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For light-inducible Cre recombinase (LiCre) expression under the 
            Gal1 promoter in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Duplus-Bottin H, Spichty M, Triqueneaux G, Place C, Mangeot PE, 
            Ohlmann T, Vittoz F, Yvert G
  TITLE     A single-chain and fast-responding light-inducible Cre recombinase 
            as a novel optogenetic switch.
  JOURNAL   Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.
  PUBMED    33620312
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-23"; volume: "10. pii: 61268. doi"; pages: " 
            10.7554/eLife.61268"
COMMENT     Sequence Label: pGY416
FEATURES             Location/Qualifiers
     source          1..7261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2754..2774
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2798..3239
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2798..2915
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3210..3233
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     3254..3270
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3254..3270
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             3380..3397
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5107..5354
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5123..5141)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5372..5391)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5373..5391)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5401..5418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5401..5417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5410..5432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5558..6013
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5645..5664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5855..5876
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6116..6790)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6791..7071)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(7092..7111)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7211..7233
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagtagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     2761 accctcacta aagggaacaa aagctggagc tctagtacgg attagaagcc gccgagcggg
     2821 tgacagccct ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc
     2881 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     2941 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga
     3001 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3061 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac
     3121 tgcataacca ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa
     3181 tgtaataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3241 aaaaaccccg gattctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct
     3301 tatcgatacc gtcgaggggc agagccgatc ctgtacactt tacttaaaac cattatctga
     3361 gtgttaaatg agaggttcac atcatcacca ccatcacggt tctttagcaa caactctgga
     3421 aagaatcgag aaaaacttcg tcatcacgga cccgaggcta cccgacaacc ctatcatttt
     3481 cgcatccgac tcttttttac aattaactga atattctaga gaagaaattc tgggccgtaa
     3541 ttgtaggttt ttacagggtc cagaaacaga tagagcaaca gttagaaaga ttagagatgc
     3601 tattgataat cagactgagg taacagttca actaataaac tatactaaat ccgggaagaa
     3661 attttggaat gtcttccact tacagccaat gagagattac aaaggtgatg tccagtattt
     3721 tatcggagtt cagttagacg gcacagaacg tctgcacggt gccgcggaga gggaagctgt
     3781 ttgtcttatt aagaaaaccg catttcaaat cgacagtgat gaggttcgca agaacctgat
     3841 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt
     3901 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
     3961 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat
     4021 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag
     4081 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
     4141 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
     4201 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta
     4261 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
     4321 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
     4381 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc
     4441 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
     4501 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
     4561 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
     4621 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
     4681 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
     4741 ggatagtgaa acaggggcaa tggtgcgcct gctggcagct ggcgattagc cattaacgcg
     4801 taaatgattg ctataattat ttgatattta tggtgacata tgagaaagga tttcaacatc
     4861 gacggaaaat atgtagtgct gtctgtaagc actaatattc agtcgccagc cgtcattgtc
     4921 actgtaaagc tgagcgatag aatgcctgat attgactcaa tatccgttgc gtttcctgtc
     4981 aaaagtatgc gtagtgctga acatttcgtg atgaatgcca ccgaggaaga agcacggcgc
     5041 ggttttgcta aagtgatgtc tgagtttggc gaactcttgg gtaaggttgg aattgtcgac
     5101 ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     5161 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     5221 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     5281 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     5341 aggctttaat ttgcggccgg tacccaattc gccctatagt gagtcgtatt aggcgcgctc
     5401 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5461 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5521 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     5581 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5641 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5701 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5761 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5821 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5881 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5941 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6001 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6061 gcatagatcg gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
     6121 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
     6181 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
     6241 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
     6301 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
     6361 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
     6421 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
     6481 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     6541 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     6601 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     6661 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
     6721 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     6781 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
     6841 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa ttaattctta
     6901 atcggcaaaa aaagaaaagc tccggatcaa gattgtacgt aaggtgacaa gctatttttc
     6961 aataaagaat atcttccact actgccatct ggcgtcataa ctgcaaagta cacatatatt
     7021 acgatgctgt tctattaaat gcttcctata ttatatatat agtaatgtcg tgatctatgg
     7081 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     7141 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7201 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7261 a
//
