LOCUS       sequence-33025-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: GFPZFN1.4-B2H
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             149..169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 caccatggac tacaaagacg atgacgaccc aaaaaagaag cgaaaggtac ccttccagtg
      181 tcgcatttgc atgcggaact tttcgacgcg gcagaagctt ggcgtgcata cccgtactca
      241 taccggtgaa aaaccgtttc agtgtcggat ctgtatgcga aatttctccg tcgcgcacaa
      301 cttgacccgc catctacgta cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat
      361 gcgcaacttc agtcagcacc ccaacctgac gaggcaccta aaaacccaca caggacagaa
      421 ggaccaacta gtcaaaagtg aactggagga gaagaaatct gaacttcgtc ataaattgaa
      481 atatgtgcct catgaatata ttgaattaat tgaaattgcc agaaattcca ctcaggatag
      541 aattcttgaa atgaaggtaa tggaattttt tatgaaagtt tatggatata gaggtaaaca
      601 tttgggtgga tcaaggaaac cggacggagc aatttatact gtcggatctc ctattgatta
      661 cggtgtgatc gtggatacta aagcttatag cggaggttat aatctgccaa ttggccaagc
      721 agatgaaatg caacgatatg tcgaagaaaa tcaaacacga aacaaacata tcaaccctaa
      781 tgaatggtgg aaagtctatc catcttctgt aacggaattt aagtttttat ttgtgagtgg
      841 tcactttaaa ggaaactaca aagctcagct tacacgatta aatcatatca ctaattgtaa
      901 tggagctgtt cttagtgtag aa
//
