LOCUS       sequence-330253-        3881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri NONO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-NONO
FEATURES             Location/Qualifiers
     source          1..3881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     370..387
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(546..573)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(665..751)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     800..822
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     814..831
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     815..831
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    847..946
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2284..2383)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2400..2419)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2401..2419)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2424..2440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2424..2440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2542..2561)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2553..3362
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2620..2639)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3509..3881,1..216)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
       61 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      241 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      301 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      361 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      481 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
      541 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
      601 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
      661 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      721 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      781 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      841 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      901 gagcaatgct tttttataat gccaactttg tacaaaaaag caggcttcga aggagataac
      961 aaaatgcaag gctacagagg cccccaacat aaccacggcc cgaatcgtcc gggagaccag
     1021 cgaggcgaag gagctaacgg aaacgggcag gagccggagc ccagtgaata catcaacccc
     1081 aatgcggcct taaccctgga cctacagagc ttcaggaagc ccggagaaaa aacgttcacc
     1141 caacggagca ggctgttcgt tggaaaccta ccgaccggaa tcaccgaaga agagctggag
     1201 aagatgttcg ccaaatacgg aaaagccggt gaggtttttg tcaacaagga gagaggtttc
     1261 ggtttcatcc ggttggagac gagaatcatt gcggagatcg ccaggtccga gctggatgat
     1321 tttgtggtca aaggccgacc catacgagtg aggtttgcta cgcacggtgc cgcattatct
     1381 gtcaagaatt tgccagagtt tgcttccaac gagctcctgg aggaggcctt cgccgtgttc
     1441 ggccaggtgg agagagctgt ggtgatagtg gatgaccgcg ggaggccaac ggggaaaggc
     1501 ttggtggagt tcacgtccaa acctgcagcg agaaaagctc tggataagtg cggtgacggt
     1561 gcctacttaa tgcagccttc ccccggccct acttaatgac agccttcccc cggcccatca
     1621 cagtggagcc tatggagcag tttgacgaag acgaggggtt gccagagaag ctgatcaaca
     1681 aaaaccaaca gttccacaaa gagcgcgagc agcctccacg gtttgctcag cctggctcgt
     1741 tcgagtacga gtacgccatg cgctggaaag ccctgatgga gatggagaag cagcagtatg
     1801 agatggtgga tcgcaacatg aaagaggctc aggagaagct ggaggctgag atggaggcag
     1861 ccagacacga gcaccaggtg atgctgatgc gtcaagacct gctgaggaga caggaggagc
     1921 tgcgaaggat ggaggagcag cacaatcaag aggtgcagaa gaggaagcag gctgagctcc
     1981 ggcaggagga ggaacgcagg aggagagagg aggaaatgat gaagaggcag caggagggct
     2041 tcaggggcgg cttctccgaa aacagagagc aggagatgag gatgcacatg gggggccatg
     2101 ggatggctat gaacagaaat tctttgggtg gaaattcggg ttctgctggt gctgctggca
     2161 taactgccga gaatactcca atgatgtcag cgtcaggaaa caacaattta ccaggaggag
     2221 gacagggggg cttccccaga ggcctcccag gctctggaga ctacaacaag cagcgcagat
     2281 ttgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2341 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2401 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2461 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     2521 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     2581 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2641 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     2701 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2761 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2821 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     2881 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2941 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     3001 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     3061 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     3121 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     3181 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     3241 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     3301 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     3361 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     3421 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3481 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3541 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3601 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3661 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3721 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3781 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3841 gcacacagcc cagcttggag cgaacgacct acaccgaact g
//
