LOCUS       sequence-33026-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Coschi CH, Martens AL, Ritchie K, Francis SM, Chakrabarti S, Berube 
            NG, Dick FA.
  TITLE     Mitotic chromosome condensation mediated by the retinoblastoma 
            protein is tumor-suppressive
  JOURNAL   Genes Dev. 2010 Jul 1;24(13):1351-63. Epub 2010 Jun 15.
  PUBMED    20551166
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2010-07-1"; volume: "24(13)"; pages: "1351-63. Epub 
            2010 Jun 15"
COMMENT     Sequence Label: pBABE-H2BGFP
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..420
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      433..442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(484..505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(745..764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggatcgtgt ggtggtcgta gaatttcgac ggtaccgcca ccatgccaga gccagcgaag
       61 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
      121 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
      181 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
      241 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
      301 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
      361 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
      421 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      481 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      541 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      601 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      661 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      721 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      781 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      841 ggcaacatcc tggggcacaa gctggagtac aactacaaca gcccacaacg tctatatcat
      901 ggccgacaag cag
//
