LOCUS       sequence-330271-       10852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of TRE3GS-->mCherry_PGK-->BFP 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_TRE3GS-->mCherry_PGK-->BFP
FEATURES             Location/Qualifiers
     source          1..10852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    174..291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        370..737
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    378..396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    414..432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    450..468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    486..504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    522..540
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    558..576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    594..612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             782..1492
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(927..945)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1195..1214
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        1523..2022
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1558..1581)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1955..1974
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2043..2744
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2840..3428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2893..2913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3311..3322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3431..3447)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3432..3448)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3538..3771
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3796..3814)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3797..3814)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4104..4123)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4337..4356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4456..4478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4516..4534)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4602..4706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4707..4775,4776..5567)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4925..4944)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5738..6326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6227..6246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6480..6497
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6567..6587)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6572..6901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6752..6887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8035..8100
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8230..8250
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8675..8809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8712..8731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8766..8785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8977..9610
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9657..9782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10279..10512
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10697..10741
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
       61 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
      121 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
      181 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      241 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      301 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt cccgtggaat
      361 tatcacctcg agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat
      421 cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag
      481 tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga
      541 acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat
      601 cagtgataga gaacgtataa gctttgctta tgtaaaccag ggcgcctata aaagagtgct
      661 gattttttga gtaaacttca attccacaac acttttgtct tataccaact ttccgtacca
      721 cttcctaccc tcgtaaagaa ttcccgtata caccggtgca attctcacgc gtgccgccac
      781 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      841 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      901 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      961 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1021 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1081 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1141 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1201 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1261 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1321 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1381 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1441 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc
     1501 ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     1561 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     1621 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     1681 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     1741 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     1801 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     1861 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     1921 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     1981 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     2041 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     2101 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     2161 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     2221 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     2281 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     2341 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     2401 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     2461 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     2521 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     2581 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     2641 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     2701 gatactgcga cctccctagc aaactggggc acaagcttaa ttaaggccgc aactcccacc
     2761 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     2821 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2881 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2941 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3001 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3061 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3121 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3181 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3241 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3301 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3361 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3421 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     3481 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3541 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3601 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3661 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3721 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     3781 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     3841 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     3901 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     3961 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     4021 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     4081 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     4141 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     4201 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     4261 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     4321 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4381 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4441 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4501 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4561 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4621 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4681 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4741 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4801 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4861 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4921 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4981 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5041 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5101 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5161 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5221 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5281 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5341 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5401 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5461 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5521 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5881 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6361 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6421 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6481 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6541 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6841 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6901 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     6961 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     7021 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     7081 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     7141 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     7201 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     7261 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     7321 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     7381 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     7441 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     7501 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     7561 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     7621 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     7681 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     7741 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     7801 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     7861 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     7921 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     7981 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     8041 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     8101 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     8161 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     8221 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     8281 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     8341 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     8401 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     8461 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     8521 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     8581 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     8641 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8701 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8761 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     8821 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     8881 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8941 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     9001 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9061 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9121 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     9181 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9241 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9301 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9361 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     9421 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9481 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9541 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9601 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9661 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9721 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9781 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9841 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9901 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9961 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10021 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10081 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
    10141 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10201 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10261 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10321 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10381 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10441 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10501 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10561 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10621 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10681 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10741 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10801 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga at
//
