LOCUS       sequence-330275-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_TRE3GS-->sGFP-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..410
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    51..69
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    87..105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    123..141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    159..177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    195..213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    231..249
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    267..285
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      449..458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(506..508,509..1219)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1235..1291
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1295..2005
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1440..1458)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1708..1727
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2103..2691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2156..2176)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2574..2585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2694..2710)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2695..2711)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2801..3034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3059..3077)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3060..3077)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3367..3386)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3600..3619)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3719..3741
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3779..3797)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3865..3969
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3970..4038,4039..4830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4188..4207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5001..5589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5490..5509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5743..5760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5830..5850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5835..6164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6015..6150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7298..7363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7493..7513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7938..8072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7975..7994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8029..8048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8240..8873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8920..9045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9542..9775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9960..10004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10289..10406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcgataagc ttgatatcga attcccgtgg aattatcacc tcgagtttac tccctatcag
       61 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
      121 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
      181 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
      241 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc
      301 ttatgtaaac cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac
      361 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcccgt
      421 atacaccggt gcaattctca cgcgtgccgc caccatgggc gttaaagtcc ttttcgccct
      481 gatctgcatt gccgtcgcag aggccgtttc taagggtgaa gaattgttca ccggagttgt
      541 gcccatcctg gttgagctgg acggagacgt taacggacac aagttctcag tgagcggcga
      601 aggagagggc gacgctacgt acggtaaact gacgttgaag ttcatctgta caacaggaaa
      661 gctgccagtg ccctggccta cactcgtgac caccctcacc tacggagtcc agtgcttctc
      721 ccggtacccc gaccacatga aacagcacga tttcttcaag tccgcgatgc ctgagggcta
      781 tgttcaggaa cgcaccatat tcttcaagga cgacggtaac tataaaacca gggctgaagt
      841 taagttcgag ggggacacgt tggtaaatag aattgagttg aaaggaatcg atttcaaaga
      901 agacggcaat attttgggcc acaagctgga gtacaactac aacagccaca acgtgtatat
      961 tatggctgat aagcagaaga acggcatcaa ggtgaacttc aaaatccgac acaatatcga
     1021 ggatggctct gtgcaactgg ccgaccatta ccagcaaaac acgcctatcg gcgacggtcc
     1081 ggttttgctg ccagataacc actacctctc cacgcaatca gccctctcca aggatcctaa
     1141 cgaaaaaaga gaccatatgg ttctgcttga attcgtgacg gctgctggga tcaccctggg
     1201 aatggatgag ctgtataagg ccagcggcag cggcgccaca aacttctctc tgctaaagca
     1261 agcaggtgat gttgaagaaa accccgggcc tcatatggtg agcaagggcg aggaggataa
     1321 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     1381 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1441 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     1501 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     1561 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     1621 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     1681 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     1741 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     1801 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     1861 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     1921 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     1981 cggcatggac gagctgtaca agtaagcggc cgcaactccc acctgcaaca tgcgtgactg
     2041 actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     2101 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2161 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2221 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2281 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     2341 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     2401 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     2461 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     2521 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     2581 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     2641 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     2701 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     2761 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     2821 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     2881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     2941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3001 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     3061 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     3121 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     3181 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     3241 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     3301 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     3361 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     3421 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     3481 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     3541 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     3601 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3661 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3721 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     3781 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3841 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3901 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3961 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4021 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4081 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4141 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4201 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4261 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4321 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4381 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4441 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4501 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4561 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4681 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4741 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4801 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5701 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5761 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5821 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5881 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6001 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6061 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6121 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
     6181 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     6241 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     6301 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     6361 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     6421 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     6481 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     6541 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     6601 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
     6661 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     6721 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
     6781 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
     6841 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
     6901 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     6961 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     7021 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     7081 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     7141 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
     7201 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     7261 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     7321 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     7381 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     7441 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     7501 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     7561 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     7621 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     7681 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     7741 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     7801 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     7861 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     7921 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8041 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     8101 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     8161 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     8221 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     8281 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     8341 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     8401 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     8461 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     8521 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     8581 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8641 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8701 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8761 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8821 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8881 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8941 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9001 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9061 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9121 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     9181 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9241 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     9301 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     9361 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     9421 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     9481 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     9541 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     9601 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     9661 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     9721 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     9781 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     9841 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     9901 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     9961 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    10021 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    10081 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    10141 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    10201 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
    10261 atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10321 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10381 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
    10441 gg
//
