LOCUS       sequence-330294-       11508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of mouse talin-1 (residues 1-2299) with C-terminal 
            deletion of dimerization domain and R13 (∆R13-DD) in mammalian 
            cells. Contains C-terminal EGFP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Azizi L, Cowell AR, Mykuliak VV, Goult BT, Turkki P, Hytonen VP
  TITLE     Cancer associated talin point mutations disorganise cell adhesion 
            and migration.
  JOURNAL   Sci Rep. 2021 Jan 11;11(1):347. doi: 10.1038/s41598-020-77911-4.
  PUBMED    33431906
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-11"; volume: "11(1):347. doi"; pages: " 
            10.1038/s41598-020-77911-4"
COMMENT     Sequence Label: pEGFP_∆R13–DD-talin1 (1-2299)
FEATURES             Location/Qualifiers
     source          1..11508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7562..7564,7565..7567,7568..8281)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7562..7564,7565..7567,7568..8278)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7607..7628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7868..7887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8215..8236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8296..8417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8333..8352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8387..8406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8424..8879)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8561..8582)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8773..8792
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8906..9010
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9012..9369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9012..9203
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9035..9055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9220..9355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9404..10198
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9404..10198
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9458..9477)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10068..10087
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10386..10405)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10430..10477
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10806..11394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11295..11314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtattag gcaccccagg ctttacacga attcgcagaa gctgccacca tggttgcgct
      661 ttcgctgaag attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat
      721 ggtgtacgac gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc
      781 caacgacttt gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc
      841 tgggaaagct ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa
      901 gcagagaccc ctgaagatcc ggatgttaga cggaacggtg aagactatca tggtggatga
      961 ctccaagacc gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca
     1021 tgatgagtat tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac
     1081 cctgagaaaa gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa
     1141 gttgcacaca gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg
     1201 agtggaagag catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt
     1261 ggattcccga gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct
     1321 gaatggctcc catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca
     1381 gatccagttt ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga
     1441 cttcctgccc aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa
     1501 gaattgtggg cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc
     1561 cctcaagact tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa
     1621 actggtgccc aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac
     1681 caaggaggtg attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa
     1741 gagctttact ctggactttg gagactatca ggatggctac tactcagtac agacgacaga
     1801 aggcgagcag atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa
     1861 aagcaaggac cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt
     1921 gtctcccaaa aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca
     1981 cggctctgtg gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca
     2041 ggtggggagc atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca
     2101 catgccacct ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca
     2161 ggctgtgcag gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca
     2221 ggatgctgcc tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca
     2281 ctcccaggta gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga
     2341 tcctgcagag acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct
     2401 gacggagatg tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa
     2461 tggccggccc cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg
     2521 cagtgctcag cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt
     2581 gggccaggcc agtggggagc tgttgcagca aattggggaa agtgacactg acccccactt
     2641 ccaggatgtt ctaatgcagc tagcaaacgc agtggcaagt gctgcagccg ccctggtcct
     2701 caaggccaag agtgtggccc agcgcacaga ggattccggg cttcagaccc aagtgattgc
     2761 tgcagctacg caatgtgctc tgtccacttc ccagctggtg gcctgtacca aggtggtagc
     2821 acctacaatc agctcacctg tgtgccaaga acagctagtc gaggctgggc gactggtggc
     2881 caaagctgtg gagggctgtg tgtctgcctc gcaagcagct acagaagatg gacagctgct
     2941 tcgaggggta ggagcagcag ccacggctgt cacccaggcc ctcaatgagc tgctgcagca
     3001 cgtgaaggcc cacgccaccg gagctgggcc tgccggtcgg tatgaccaag ccactgatac
     3061 catcctcacc gtcactgaaa acatcttcag ctccatgggt gatgctgggg agatggtgcg
     3121 ccaggcccgc atcctggccc aagccacctc agacctggtc aatgctatca aggccgatgc
     3181 tgagggggag agtgatctgg agaactctag aaagctcctg agtgctgcca agatcctcgc
     3241 tgatgccacc gccaagatgg tggaggcggc caagggagca gccgcccacc ctgacagtga
     3301 ggaacagcag cagcgactgc gtgaagcagc tgaggggctt cgcatggcca ccaatgcagc
     3361 tgcgcagaac gccatcaaga agaagttggt gcagcgcctg gagcatgcag ccaagcaagc
     3421 tgcagcctct gcaacacaga ccattgctgc agcccaacat gcagcctctg cccccaaggc
     3481 ctctgcgggc ccccagcccc tactggtgca gagctgtaag gccgtggcag agcagattcc
     3541 gttgctggtg cagggtgtcc gaggaagtca agctcagcct gacagcccta gtgctcagct
     3601 cgccctcatt gctgccagcc agagcttcct gcagccaggt gggaagatgg tggcagcggc
     3661 aaaagcctca gtgccaacaa ttcaggacca ggcttcagcg atgcagctga gtcagtgcgc
     3721 aaagaaccta ggcactgccc tggccgagct gcgcactgct gctcagaagg ctcaggaggc
     3781 atgtggacct ttggagatgg attctgcact gagtgttgta caaaatctag agaaagacct
     3841 acaggaaata aaagcagcag ctcgagatgg caagcttaag cccttacctg gggaaacaat
     3901 ggagaagtgt acccaagatc ttggcaacag caccaaagcg gtgagctctg ccatcgccaa
     3961 gctgctgggg gagattgccc agggcaacga gaactatgca ggtattgcag ctcgggatgt
     4021 agctggtgga ctaaggtcac tagcccaggc tgcgcggggt gtggctgcgc tgacatcaga
     4081 tcctgcagtg caggccattg tgcttgacac agccagcgac gttctggaca aggccagcag
     4141 cctcattgaa gaggcaaaaa aagcatctgg acacccaggg gacccagaaa gccagcaaag
     4201 gcttgctcag gtagctaaag cagtgaccca agccctgaac cgctgtgtca gctgcctgcc
     4261 tggccaaaga gacgtggata atgccctacg agccgttgga gatgccagca agcgactcct
     4321 gagtgacttg cttcctccaa gcacggggac atttcaagaa gcacagagcc gattgaatga
     4381 agctgcagct gggttaaatc aggccgccac agaactggtg caagcctccc gaggaactcc
     4441 tcaggacctg gctcgggcct ccggccgatt tggacaggac ttcagcacct tcctggaagc
     4501 tggtgtggag atggccggac aggccccgag ccaggaggac cgagcccagg tggtgtccaa
     4561 cctgaagggc atatccatgt cttcaagcaa acttctcctt gccgctaagg ccttgtccac
     4621 agaccctgct tctcccaacc tcaagagtca gctggctgcg gctgcccggg cagtgacgga
     4681 cagcatcaac cagctcatca ccatgtgcac ccagcaggca cctggccaga aggagtgtga
     4741 caatgcactt cggcagctag agacagtccg agaactcctg gagaatccag tccagcccat
     4801 caacgacatg tcctacttcg gttgcttgga cagtgtcatg gagaactcca aggtcctagg
     4861 tgaggccatg actggcatct cccaaaatgc caagaatgga aatctgccgg agtttggaga
     4921 tgccattgcc acagcctcca aggctctctg tggcttcacg gaggcagccg cacaggcagc
     4981 atatctagtt ggtgtctctg accccaacag ccaagctgga cagcaaggac tggtggaacc
     5041 cacacagttt gcccgtgcaa accaggcaat tcagatggcc tgtcagagtc tgggggagcc
     5101 tggctgtacc caggcccagg tgttatctgc agccactatt gtagccaaac acacatctgc
     5161 attgtgtaac agctgtcgcc tggcttccgc taggactgcc aatcccactg ccaagcgcca
     5221 gtttgtacag tcagccaagg aggtggccaa cagtacagcc aatcttgtca agaccatcaa
     5281 ggcactagat ggggacttca cagaagagaa ccgtgcccag tgccgagcag ccacagcccc
     5341 tctgctggaa gctgtggata acctgagtgc ctttgcctcc aaccctgagt tctccagcgt
     5401 ccctgcccag atcagccctg agggcagagc agccatggag cccattgtaa tctctgctaa
     5461 gacaatgttg gagagtgctg gagggctcat ccagacagct cgggcgttag cagtcaatcc
     5521 ccgagacccc ccacgttggt ctgtgctagc tggccactct cggactgtct cagattccat
     5581 caagaaactt attacaagca tgagggacaa agccccaggg cagctggagt gtgagacagc
     5641 cattgcggct ctgaacagct gcttgcggga cctagatcag gcttcgcttg ccgctgtcag
     5701 ccagcagctt gctccccgtg aaggaatctc tcaagaggct ttgcacaccc agatgctcac
     5761 tgcagtgcaa gaaatttctc atctcattga gccgctggcc agcgctgctc gggctgaagc
     5821 ctcccagctg ggacacaagg tgtcccaaat ggcccagtac tttgaaccac tcaccctggc
     5881 tgcagtgggt gctgcgtcta agaccctgag ccacccgcag cagatggcac ttctggacca
     5941 gactaaaacg ttggcagagt ctgccttgca gttgctatat actgccaagg aggctggtgg
     6001 taaccccaag caagcagcac acacccagga agccctggag gaggctgtgc agatgatgac
     6061 agaggccgta gaagacctga cgacgaccct caacgaagca gccagtgctg caggagtcgt
     6121 tggcggcatg gtggactcta tcactcaggc catcaaccag ctagatgaag gacctatggg
     6181 tgacccagaa ggctcattcg tagattacca gacaaccatg gtgaggacag ccaaggccat
     6241 tgctgtcact gttcaggaga tggtaaccaa gtcaaacacc agccctgaag agctaggccc
     6301 tcttgccaac cagctgacca gtgactatgg ccgactggcc tcacaagcca agcctgcagc
     6361 tgtggctgct gaaaatgaag agataggcgc tcacatcaaa caccgagtac aggagctggg
     6421 ccatggctgc tctgctctgg tcaccaaggc aggtgccttg cagtgtagcc ccagtgatgt
     6481 ctacaccaag aaggagctca tagagtgtgc ccgcagagtg tcagagaagg tctcccatgt
     6541 tctggctgca ctccaggctg ggaatcgtgg tacccaggcc tgcattacag cagccagtgc
     6601 tgtgtctggt atcattgctg acctcgacac caccatcatg ttcgctactg ctggcacact
     6661 taaccgtgag ggtgctgaaa cttttgctga ccaccgggag ggtatcttaa agacagcaaa
     6721 ggttcttgtg gaggacacca aggtcctagt gcagaatgca gctgggagcc aggagaagtt
     6781 ggcacaagcc gcccagtcct ccgtggccac cattacccgc ctcgctgatg tggtcaagct
     6841 cggtgcagcc agcctaggag ccgaagaccc tgaaactcag gtggtgctga tcaatgcagt
     6901 aaaggacgta gccaaggccc tgggtgacct catcagcgct acgaaggctg cagcgggcaa
     6961 agttggggat gaccctgcag tgtggcagct caagaactct gccaaggtga tggtgaccaa
     7021 tgtgacatca ttgctcaaga cagtgaaggc tgtggaagat gaggccacca aaggcacacg
     7081 ggccctagag gcaaccacag agcacatacg tcaggaactg gcggtcttct gttccccaga
     7141 gccacctgcc aagacctcta cccctgaaga cttcatccga atgaccaagg gtattactat
     7201 ggcaacagcc aaagccgttg ctgctggcaa ttcctgtcga caggaagatg tcattgccac
     7261 agccaatctg agccgacggg ctattgcgga catgcttcgg gcttgcaagg aagcagcttt
     7321 ccacccagaa gtggcgcctg atgtacggct ccgagccctg cactatggcc gggagtgtgc
     7381 caatggttac ctggaactgc tggaccacgt gctgctgacc cttcagaagc caaacccaga
     7441 cctgaagcag cagctaactg gacactcaaa gcgggttgct ggctctgtga ctgagctcat
     7501 ccaggctgct gaagccatga aaggaacaga gtgggtggac ccagaggacg gaggatccgg
     7561 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     7621 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7681 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     7741 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     7801 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     7861 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     7921 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     7981 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     8041 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     8101 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     8161 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     8221 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     8281 acaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8401 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     8461 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     8521 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     8581 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     8641 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     8701 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     8761 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     8821 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     8881 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8941 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9001 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     9061 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9361 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     9421 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     9481 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     9541 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     9601 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     9661 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     9721 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     9781 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     9841 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     9901 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9961 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    10021 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    10081 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    10141 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    10201 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
    10261 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    10321 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
    10381 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
    10441 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
    10501 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
    10561 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
    10621 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
    10681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10921 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11461 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
