LOCUS       sequence-330317-        9593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid encodes a Cas9 protein as well as two sgRNAs, one 
            targets the C-terminus of Whi5 and the other targets the C-terminus 
            of Hta2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9593)
  TITLE     Heinemann Lab Plasmids
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HCP7
FEATURES             Location/Qualifiers
     source          1..9593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             862..4965
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             862..4965
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4978..4998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5002..5022
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_RNA        5582..5657
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6053..6128
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      7216..7413
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    7418..7921
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(7965..8780)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7965..8780)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8688..8707
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8772..8791
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9005..9593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9494..9513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcccc tgaattcgca
      121 tctagactga actggccgat aattgcagac gaacggtgag taaggaaaga gtgaggaact
      181 atcgcatacc tgcatttaaa gatgccgatt tgggcgcgaa tcctttattt tggcttcacc
      241 ctcatactat tatcagggcc agaaaaagga agtgtttccc tccttcttga attgatgtta
      301 ccctcataaa gcacgtggcc tcttatcgag aaagaaatta ccgtcgctcg tgatttgttt
      361 gcaaaaagaa caaaactgaa aaaacccaga cacgctcgac ttcctgtcat cctattgatt
      421 gcagcttcca atttcgtcac acaacaaggt cctagcgacg gctcacaggt tttgtaacaa
      481 gcaatcgaag gttctggaat ggcgggaaag ggtttagtac cacatgctat gatgcccact
      541 gtgatctcca gagcaaagtt cgttcgatcg tactgttact ctctctcttt caaacagaat
      601 tgtccgaatc gtgtgacaac aacagcctgt tctcacacac tcttttcttc taaccaaggg
      661 ggtggtttag tttagtagaa cctcgtgaaa cttacattta catatatata aacttgcata
      721 aattggtcaa tgcaagaaat acatatttgg tcttttctaa ttcgtagttt ttcaagttct
      781 tagatgcttt ctttttctct tttttacaga tcatcaagga agtaattatc tactttttac
      841 aacaaatata aaacaagatc tatggacaag aagtattcta tcggactgga tatcgggact
      901 aatagcgtcg ggtgggccgt gatcactgac gagtacaagg tgccctctaa gaagttcaag
      961 gtgctcggga acaccgaccg gcattccatc aagaaaaatc tgatcggagc tctcctcttt
     1021 gattcagggg aaaccgctga agcaacccgc ctcaagcgga ctgctagacg gcggtacacc
     1081 aggaggaaga accggatttg ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc
     1141 gacgacagct tcttccatag gctggaagaa tcattcctcg tggaagagga taagaagcat
     1201 gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg cctatcacga gaaataccca
     1261 accatctacc atcttcgcaa aaagctggtg gactcaaccg acaaggcaga cctccggctt
     1321 atctacctgg ccctggccca catgatcaag ttcagaggcc acttcctgat cgagggcgac
     1381 ctcaatcctg acaatagcga tgtggataaa ctgttcatcc agctggtgca gacttacaac
     1441 cagctctttg aagagaaccc catcaatgca agcggagtcg atgccaaggc cattctgtca
     1501 gcccggctgt caaagagccg cagacttgag aatcttatcg ctcagctgcc gggtgaaaag
     1561 aaaaatggac tgttcgggaa cctgattgct ctttcacttg ggctgactcc caatttcaag
     1621 tctaatttcg acctggcaga ggatgccaag ctgcaactgt ccaaggacac ctatgatgac
     1681 gatctcgaca acctcctggc ccagatcggt gaccaatacg ccgacctttt ccttgctgct
     1741 aagaatcttt ctgacgccat cctgctgtct gacattctcc gcgtgaacac tgaaatcacc
     1801 aaggcccctc tttcagcttc aatgattaag cggtatgatg agcaccacca ggacctgacc
     1861 ctgcttaagg cactcgtccg gcagcagctt ccggagaagt acaaggaaat cttctttgac
     1921 cagtcaaaga atggatacgc cggctacatc gacggaggtg cctcccaaga ggaattttat
     1981 aagtttatca aacctatcct tgagaagatg gacggcaccg aagagctcct cgtgaaactg
     2041 aatcgggagg atctgctgcg gaagcagcgc actttcgaca atgggagcat tccccaccag
     2101 atccatcttg gggagcttca cgccatcctt cggcgccaag aggacttcta cccctttctt
     2161 aaggacaaca gggagaagat tgagaaaatt ctcactttcc gcatccccta ctacgtggga
     2221 cccctcgcca gaggaaatag ccggtttgct tggatgacca gaaagtcaga agaaactatc
     2281 actccctgga acttcgaaga ggtggtggac aagggagcca gcgctcagtc attcatcgaa
     2341 cggatgacta acttcgataa gaacctcccc aatgagaagg tcctgccgaa acattccctg
     2401 ctctacgagt actttaccgt gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg
     2461 atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg caattgtgga ccttctgttc
     2521 aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa
     2581 tgcttcgact ctgtggagat tagcggggtc gaagatcggt tcaacgcaag cctgggtact
     2641 taccatgatc tgcttaagat catcaaggac aaggattttc tggacaatga ggagaacgag
     2701 gacatccttg aggacattgt cctgactctc actctgttcg aggaccggga aatgatcgag
     2761 gagaggctta agacctacgc ccatctgttc gacgataaag tgatgaagca acttaaacgg
     2821 agaagatata ccggatgggg acgccttagc cgcaaactca tcaacggaat ccgggacaaa
     2881 cagagcggaa agaccattct tgatttcctt aagagcgacg gattcgctaa tcgcaacttc
     2941 atgcaactta tccatgatga ttccctgacc tttaaggagg acatccagaa ggcccaagtg
     3001 tctggacaag gtgactcact gcacgagcat atcgcaaatc tggctggttc acccgctatt
     3061 aagaagggta ttctccagac cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc
     3121 cataaaccag agaacattgt catcgagatg gccagggaaa accagactac ccagaaggga
     3181 cagaagaaca gcagggagcg gatgaaaaga attgaggaag ggattaagga gctcgggtca
     3241 cagatcctta aagagcaccc ggtggaaaac acccagcttc agaatgagaa gctctatctg
     3301 tactaccttc aaaatggacg cgatatgtat gtggaccaag agcttgatat caacaggctc
     3361 tcagactacg acgtggacca tatcgtccct cagagcttcc tcaaagacga ctcaattgac
     3421 aataaggtgc tgactcgctc agacaagaac cggggaaagt cagataacgt gccctcagag
     3481 gaagtcgtga aaaagatgaa gaactattgg cgccagcttc tgaacgcaaa gctgatcact
     3541 cagcggaagt tcgacaatct cactaaggct gagaggggcg gactgagcga actggacaaa
     3601 gcaggattca ttaaacggca acttgtggag actcggcaga ttactaaaca tgtcgcccaa
     3661 atccttgact cacgcatgaa taccaagtac gacgaaaacg acaaacttat ccgcgaggtg
     3721 aaggtgatta ccctgaagtc caagctggtc agcgatttca gaaaggactt tcaattctac
     3781 aaagtgcggg agatcaataa ctatcatcat gctcatgacg catatctgaa tgccgtggtg
     3841 ggaaccgccc tgatcaagaa gtacccaaag ctggaaagcg agttcgtgta cggagactac
     3901 aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc aggagatcgg aaaggccacc
     3961 gcaaagtact tcttctacag caacatcatg aatttcttca agaccgaaat cacccttgca
     4021 aacggtgaga tccggaagag gccgctcatc gagactaatg gggagactgg cgaaatcgtg
     4081 tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac
     4141 atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta aggaatcaat cctccccaag
     4201 cgcaactccg acaagctcat tgcaaggaag aaggattggg accctaagaa gtacggcgga
     4261 ttcgattcac caactgtggc ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag
     4321 tctaagaagc tcaagagcgt gaaggaactg ctgggtatca ccattatgga gcgcagctcc
     4381 ttcgagaaga acccaattga ctttctcgaa gccaaaggtt acaaggaagt caagaaggac
     4441 cttatcatca agctcccaaa gtatagcctg ttcgaactgg agaatgggcg gaagcggatg
     4501 ctcgcctccg ctggcgaact tcagaagggt aatgagctgg ctctcccctc caagtacgtg
     4561 aatttcctct accttgcaag ccattacgag aagctgaagg ggagccccga ggacaacgag
     4621 caaaagcaac tgtttgtgga gcagcataag cattatctgg acgagatcat tgagcagatt
     4681 tccgagtttt ctaaacgcgt cattctcgct gatgccaacc tcgataaagt ccttagcgca
     4741 tacaataagc acagagacaa accaattcgg gagcaggctg agaatatcat ccacctgttc
     4801 accctcacca atcttggtgc ccctgccgca ttcaagtact tcgacaccac catcgaccgg
     4861 aaacgctata cctccaccaa agaagtgctg gacgccaccc tcatccacca gagcatcacc
     4921 ggactttacg aaactcggat tgacctctca cagctcggag gggatgaggg agctgatcca
     4981 aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg tataaggatc ctaactcgag
     5041 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
     5101 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
     5161 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
     5221 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgc caaaccagat
     5281 gtcaacacag ctacaacgat gtgcttcagt attacatttt ttgccttcaa cgccttgatt
     5341 gttctatttt tgctaataat aaatctattt catcggacta aaagtccatt agttgtaagc
     5401 ggatttagct cagttgggag agcgccagac tgaagaaaaa cttcggtcaa gtcatctgga
     5461 ggtcctgtgt tcgatccaca gaattcgcag atggccggca tggtcccagc ctcctcgctg
     5521 gcgccggctg ggcaacacct tcgggtggcg aatgggactt tctccacttc ggtatccgac
     5581 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     5641 tggcaccgag tcggtgcttt ttttattttt tgtcactatt gttatgtaaa atgccacctc
     5701 tgacagtatg gaacgcaaac ttctgtctag tggatagctg gatgcacaca ctggcttaag
     5761 atgacaacga tgtgcttcag tattacattt tttgccttca acgccttgat tgttctattt
     5821 ttgctaataa taaatctatt tcatcggact aaaagtccat tagttgtaag cggatttagc
     5881 tcagttggga gagcgccaga ctgaagaaaa acttcggtca agtcatctgg aggtcctgtg
     5941 ttcgatccac agaattcgca gatggccggc atggtcccag cctcctcgct ggcgccggct
     6001 gggcaacacc ttcgggtggc gaatgggact ttttgagaag ctttggcagt ctgttttaga
     6061 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     6121 gtcggtgctt tttttatttt ttgtcactat tgttatgtaa aatgccacct ctgacagtat
     6181 ggaacgcaaa cttctgtcta gtggatagct gagcagttac agagatgtta cgaaccacta
     6241 gtgcactgca gtacagttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     6301 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     6361 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     6421 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tccagacctg cgagcaggga
     6481 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     6541 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     6601 ggatacagtt ctcacatcac atccgaacat aaacaaaaat gggtactacc ttagatgata
     6661 cagcctacag atacagaaca tcagtccctg gtgatgctga agcaattgag gctttagacg
     6721 gttcattcac caccgacacc gtctttagag taaccgccac cggtgatgga tttaccttaa
     6781 gagaagtccc agtcgaccct ccattaacta aagtctttcc agatgatgaa tctgatgacg
     6841 aaagcgacga cggagaagat ggtgacccag attcaagaac tttcgtagca tacggtgatg
     6901 acggtgattt ggctggtttt gtagtcgttt cttattcagg ttggaataga aggttgaccg
     6961 ttgaagatat agaagtcgcc ccagagcata gaggtcatgg tgtaggaaga gctttgatgg
     7021 gtttggctac agaatttgca agagagagag gagccggtca tttatggtta gaagttacta
     7081 atgttaacgc ccctgctatc catgcttata gaagaatggg tttcacatta tgtggtttag
     7141 atactgcttt atatgatgga acagcatctg acggtgaaca ggccttgtat atgtctatgc
     7201 cttgccctta aagtaactga caataaaaag attcttgttt tcaagaactt gtcatttgta
     7261 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     7321 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     7381 gtcaatcgta tgtgaatgct ggtcgctata ctggagtatc acgtgctata aaaataatta
     7441 taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta
     7501 aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa
     7561 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
     7621 tctgtgtaga aaaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
     7681 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
     7741 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
     7801 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
     7861 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat
     7921 ccgtcccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     7981 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     8041 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     8101 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     8161 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     8221 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     8281 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga
     8341 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     8401 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     8461 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     8521 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     8581 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     8641 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     8701 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt
     8761 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     8821 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     8881 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc atgaccaaaa
     8941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaa
//
