LOCUS       sequence-330335-        9779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-inducible lentiviral vector expressing hM3Dq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Klawonn AM, Fritz M, Castany S, Pignatelli M, Canal C, Simila F, 
            Tejeda HA, Levinsson J, Jaarola M, Jakobsson J, Hidalgo J, Heilig M,
            Bonci A, Engblom D
  TITLE     Microglial activation elicits a negative affective state through 
            prostaglandin-mediated modulation of striatal neurons.
  JOURNAL   Immunity. 2021 Feb 9;54(2):225-234.e6. doi: 
            10.1016/j.immuni.2020.12.016. Epub 2021 Jan 20.
  PUBMED    33476547
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.12.016"; journalName: "Immunity"; date: 
            "2021-02-9- 9"; volume: "54"; issue: "2"; pages: "225-234.e6"
COMMENT     Sequence Label: pLV.CMV.flex.hM3Dq.Cherry
FEATURES             Location/Qualifiers
     source          1..9779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3115..3240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3733..3966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4151..4195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        4474..4777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4778..4981
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    5066..5099
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    5162..5195
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5208..5918)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5486..5505)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5755..5773
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5915..5924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7728..7761)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7832..7865)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7942..7962)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8360..8371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8520..8753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8825..8946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8862..8881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8916..8935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8965..9100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9132..9151)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9133..9151)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9161..9178)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9161..9177)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9170..9192)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9322..9777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9409..9428)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9619..9640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct
     3121 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3181 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3241 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3301 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3361 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3421 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3481 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3541 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3601 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3661 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3721 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3781 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3841 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3901 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3961 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     4021 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4081 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4141 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4201 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4261 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4321 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     4381 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     4441 atctcgacgg tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     4501 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4561 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4621 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4681 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     4741 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     4801 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4861 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4921 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     4981 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     5041 aagacaccga ctctagagga tccccataac ttcgtataaa gtatcctata cgaagttata
     5101 tcaaaatagg aagaccaatg cttcaccatc gacccgaatt gccaagcatc accatcgacc
     5161 cataacttcg tataatgtat gctatacgaa gttattgtac aaccggttta cttgtacagc
     5221 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
     5281 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
     5341 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
     5401 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
     5461 tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
     5521 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg
     5581 gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg
     5641 gggaaggaca gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag
     5701 ccgtacatga actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc
     5761 accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc
     5821 tcgaactcgt ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg
     5881 atgatggcca tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg gggatccttc
     5941 aaggcctgct cgggtgcgcg cttgtgaaaa atgaccgact gtctctgctg gtactgctgc
     6001 ttgcgcctct tttttttgtc acactggcac agcagcagca tcttgaaagt ggttctgaat
     6061 gttttgttgc acagagcata gcacacgggg ttcacggtgc tgttgatgta gcacagccag
     6121 tagcccagat tccaaaaggt tttgggtatg cagctgtcac aaaaggtgtt caccagaacc
     6181 atgatgttgt atggggtcca agtgatgatg aaggcaagca agatcgcact gagggtctgg
     6241 gccgctttct tctccttgac cagggacatc cttttccgct tagtgatctg acttctggtc
     6301 ttcagagcaa acctcttggc cagagtggct tccttgaagg acagaggtag agtggccgtg
     6361 ctcttaccca ctgaggagtt gacgtcagaa gtcttagctg tgtccacggc tgactctagc
     6421 tggatgggaa gcttggagaa gctttttgga aaactgcctc catcgtccac gctcttctgg
     6481 gcctgcagct tgtcggcttt cctctccaag tccaccatcc ccagctcctc ctcaggcacc
     6541 tgcaggttgt ccgatgaggg taacttggtg gagttgagga tggtgctgtg acccggaagc
     6601 ttgagcacga tggagtagat ggctctcgtc tcggagccaa tgtcctcctc gtcggaggag
     6661 gcggagttct ccagggaggc agcagcatca ttgttgttcc aactgtcact gctgctgtgg
     6721 tcttggtcca tctgctcgga gctgggtttc cagctcttgg ttgtgaacca gaagtggcag
     6781 cggccatact tcctcctgtt ggagcgtttc atgctttgct gttgaagttc gtaactgctg
     6841 cagcttcgag aactgcccgt ggggtggaca aagttttctg tctctgcctc tgtcccagag
     6901 gcttgcaggc cagcaagctc tttggtacgc ttttcagttt ccttatagat cctccagtat
     6961 aaaatagtca taatggtgac aggcatataa aaaccagcga tggctgtgcc aaaagtaatg
     7021 gtgggctcac tgaggaactg aatgaagcac tctcccggag gcacagttct ctttccaaca
     7081 aagtattgcc agaacaagat ggcaggagcc caaaggacaa aggagatgac ccaagccaga
     7141 ccgatcatca caccggctct ctttgttgtt cgtttggctc ggtacgtgag cggcctcgtg
     7201 atggaaaagt atctgtcaaa gctgatgacc agaagattca taacagaggc attgctggct
     7261 acgcagtcaa tggcaagcca gaggtcacag gccaagttcc ctaaggccca tcgattcatg
     7321 atgatgtagg tcgtaaacag attcattgaa atgaccccga taatcagatc ggcacaggcc
     7381 aggcttaaga ggaagtagtt gttgaccgtc ttcagctgct tgttgacctt aaatgacaca
     7441 attaccagga tgttgccgat gatggtcacc aaggccagga tgcccgttaa gaaagcgatg
     7501 aagaccactt gccagacggt atgacctccc agagggtcat cggtggtacc gtctggagag
     7561 gagaaattgc cagctgctcg agaaacattg tagctgccga aatgagtgac ggttcccggg
     7621 ggcagccctg catcggaggg gctgtgtatc caggaggagc tgatgtttgg aaacaaaggc
     7681 gaggttgtac tgttattgtg caaggtcatg gtggcgctag caccggtata acttcgtata
     7741 ggatacttta tacgaagtta tcattgggat tcttcctatt ttgatccaag catcaccatc
     7801 gaccctctag tccagatctc accatcgacc cataacttcg tatagcatac attatacgaa
     7861 gttatgtccc tcgaagaggt tcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7981 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8041 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8101 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8161 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8221 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8281 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8341 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8401 gccggctcta gagcctcttc cgcgtcttcg ccttcccggg tcgagctcgg tacctttaag
     8461 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8521 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     8581 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8641 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8701 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8761 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8821 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8941 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
     9001 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9061 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg cgtcgagacg
     9121 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     9181 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9241 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9301 gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9361 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9421 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9481 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9541 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9601 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9661 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9721 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcc
//
