LOCUS       sequence-330351-        9545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal inducible expression of D10A-Cas9 nickase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  TITLE     Burgess Lab Plasmids
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPH3
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(138..156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    197..700
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        737..841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(842..910,911..1702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1060..1079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1873..2461
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2362..2381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2615..2632
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2749..2770
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2785..2790,2791..2808,2809..2815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2823..2839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2828..2850
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2847..2863
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2847..2863
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2882..2902
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2884..2902
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3311..3334
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(3367..3393,3394..3396,3397..7470)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7483..7503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7519..7766
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7535..7553)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7784..7803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7785..7803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7813..7830)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7813..7829)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7822..7844)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7970..8425
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8057..8076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8267..8288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8528..9202)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(9203..9483)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(9504..9523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
       61 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      121 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      181 aatggtttct tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
      241 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
      301 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
      361 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
      421 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
      481 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag
      541 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
      601 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
      661 ttaaaaaatt taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac
      721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      841 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      901 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      961 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1021 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1081 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1141 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1201 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1261 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1321 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1381 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1441 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1501 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1561 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1621 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1681 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1741 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1801 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1861 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1921 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1981 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2041 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2101 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2161 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2221 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2281 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2341 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2401 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2461 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2521 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2581 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2641 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2701 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2761 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2821 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     2881 cgcaattaac cctcactaaa gggaacaaaa gctggagctc gtgacagccc tccgaaggaa
     2941 gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca gatgtgcctc
     3001 gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg ttatgaagag
     3061 gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg aacgaatcaa attaacaacc
     3121 ataggatgat aatgcgatta gttttttagc cttatttctg gggtaattaa tcagcgaagc
     3181 gatgattttt gatctattaa cggatatata aatgcaaaaa ctgcataacc actttaacta
     3241 atactttcaa cattttcggt ttgtattact tcttattcaa atgtaataaa agtatcaaca
     3301 aaaaattgtt aatatacctc tatactttaa cgtcaaggag aaaaaacccc ggattctaga
     3361 actagtatgg ataagaaata ctctattggt ttggctatcg gtacaaactc tgttggttgg
     3421 gctgttatta ctgatgaata caaggttcca tccaagaagt tcaaggtttt gggtaacact
     3481 gatagacact ccatcaaaaa gaacttgatt ggtgccttgt tgttcgattc tggtgaaact
     3541 gctgaagcta ctagattgaa aagaaccgct agaagaagat acaccagaag aaagaacaga
     3601 atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc
     3661 cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc
     3721 tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg
     3781 aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg
     3841 gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac
     3901 agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag
     3961 aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa
     4021 tcccggcggc tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt
     4081 ggtaatctta tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg
     4141 gccgaagatg ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg
     4201 ctggcccaga tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac
     4261 gccattctgc tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc
     4321 gctagtatga tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt
     4381 gtcagacagc aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc
     4441 tacgccggat acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc
     4501 atcttggaaa aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg
     4561 ttgcgcaaac agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa
     4621 ctgcacgcta tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa
     4681 aagattgaga aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga
     4741 aattccagat tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc
     4801 gaggaagtcg tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt
     4861 gataaaaatc tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc
     4921 acagtttata acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca
     4981 ttcctgtctg gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa
     5041 gttaccgtga aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt
     5101 gaaatcagcg gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg
     5161 aaaatcatta aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac
     5221 attgtcctca cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact
     5281 tacgctcatc tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga
     5341 tgggggcggc tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca
     5401 atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat
     5461 gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac
     5521 agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg
     5581 cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat
     5641 atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg
     5701 gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa
     5761 cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac
     5821 ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg
     5881 gatcatatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca
     5941 agatccgata aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa
     6001 atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat
     6061 aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa
     6121 aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc
     6181 atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg
     6241 aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc
     6301 aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc
     6361 aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt
     6421 aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt
     6481 tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg
     6541 aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg
     6601 gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc
     6661 gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag
     6721 ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca
     6781 gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa
     6841 agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc
     6901 atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt
     6961 cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc
     7021 gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg
     7081 gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc
     7141 gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa
     7201 agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg
     7261 gataagccca tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg
     7321 ggcgcgcctg cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct
     7381 acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca
     7441 agaatcgacc tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag
     7501 gtgtgatctc ttctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
     7561 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     7621 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     7681 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     7741 tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata gtgagtcgta
     7801 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7861 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7921 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt
     7981 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8041 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8101 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8161 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8221 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8281 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8341 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8401 aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     8461 gtatttcaca ccgcatagat cggcaagtgc acaaacaata cttaaataaa tactactcag
     8521 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac
     8581 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat
     8641 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt tcggggctct
     8701 cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct gtcccacctg
     8761 cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca ctgagtagta
     8821 tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg aactcttggt
     8881 attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa tcattgacca
     8941 gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat ttcggagtgc
     9001 ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata accgggtcaa
     9061 ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg gaatctagag
     9121 cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac
     9181 ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc acgtatactc
     9241 acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt ttttcgaccg
     9301 aattaattct taatcggcaa aaaaagaaaa gctccggatc aagattgtac gtaaggtgac
     9361 aagctatttt tcaataaaga atatcttcca ctactgccat ctggcgtcat aactgcaaag
     9421 tacacatata ttacgatgct gttctattaa atgcttccta tattatatat atagtaatgt
     9481 cgtgatctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     9541 cgaca
//
