LOCUS       sequence-330354-        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal inducible expression of Cas9 WT .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  TITLE     Burgess Lab Plasmids
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRR1
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     367..384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    501..522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(537..542,543..560,561..567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    575..591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     580..602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     599..615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     599..615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     634..654
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        636..654
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1063..1086
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1107..1123
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1107..1123
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1137..5240
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5253..5273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5289..5536
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5305..5323)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5554..5573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5555..5573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5583..5600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5583..5599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5592..5614)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5740..6195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5827..5846)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6037..6058
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6298..6972)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6973..7253)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(7274..7293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7393..7415
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7453..7471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7512..8015
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8052..8156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8157..8225,8226..9017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8375..8394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9188..9563,1..213)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      481 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      541 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      601 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
      661 aagctggagc tcgtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca
      721 ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat
      781 tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca
      841 aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta
      901 gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacggatata
      961 taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg gtttgtatta
     1021 cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc tctatacttt
     1081 aacgtcaagg agaaaaaacc ccggattcta gaactagtgg atcccccggg aaaaaaatgg
     1141 acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggttgg gccgtcatta
     1201 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
     1261 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca
     1321 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
     1381 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
     1441 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
     1501 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
     1561 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
     1621 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg
     1681 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca
     1741 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc
     1801 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta
     1861 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg
     1921 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga
     1981 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc
     2041 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga
     2101 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc
     2161 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat
     2221 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa
     2281 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac
     2341 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta
     2401 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga
     2461 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat
     2521 tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc gaggaagtcg
     2581 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc
     2641 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata
     2701 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg
     2761 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga
     2821 aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg
     2881 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta
     2941 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca
     3001 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc
     3061 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc
     3121 tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt
     3181 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc
     3241 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg
     3301 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta
     3361 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg
     3421 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga
     3481 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg
     3541 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca
     3601 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg
     3661 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata
     3721 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt
     3781 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta
     3841 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg
     3901 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca
     3961 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc
     4021 tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc
     4081 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc
     4141 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga
     4201 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata
     4261 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac
     4321 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga
     4381 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga
     4441 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac
     4501 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca
     4561 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg
     4621 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc
     4681 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact
     4741 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga
     4801 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact
     4861 atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc gtggaacaac
     4921 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc
     4981 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca
     5041 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg
     5101 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg
     5161 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc
     5221 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgatctc
     5281 ttctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
     5341 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
     5401 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
     5461 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
     5521 gaaggcttta atttgcggcc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5581 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5641 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5701 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     5761 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5821 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5881 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5941 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6001 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6061 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6121 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6181 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6241 ccgcatagat cggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta
     6301 tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc
     6361 cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt
     6421 ttctggcgtc agtccaccag ctaacataaa atgtaagctt tcggggctct cttgccttcc
     6481 aacccagtca gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc
     6541 aaacaaggga ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc
     6601 ttttggaaat acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca
     6661 cgactcatct ccatgcagtt ggacgatatc aatgccgtaa tcattgacca gagccaaaac
     6721 atcctcctta ggttgattac gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt
     6781 tttatatgct tttacaagac ttgaaatttt ccttgcaata accgggtcaa ttgttctctt
     6841 tctattgggc acacatataa tacccagcaa gtcagcatcg gaatctagag cacattctgc
     6901 ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt
     6961 aataacagac atactccaag ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa
     7021 tagtcaccaa tgccctccct cttggccctc tccttttctt ttttcgaccg aattaattct
     7081 taatcggcaa aaaaagaaaa gctccggatc aagattgtac gtaaggtgac aagctatttt
     7141 tcaataaaga atatcttcca ctactgccat ctggcgtcat aactgcaaag tacacatata
     7201 ttacgatgct gttctattaa atgcttccta tattatatat atagtaatgt cgtgatctat
     7261 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     7321 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7381 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7441 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     7501 tttcttagac ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa
     7561 taatttggga atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa
     7621 gtaaataaag aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa
     7681 aaatttcaac aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa
     7741 tagatataca ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt
     7801 cttctacaca gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga
     7861 taaaaggtag tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa
     7921 ctattttttc tttaatttct ttttttactt tctattttta atttatatat ttatattaaa
     7981 aaatttaaat tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc
     8041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9541 gaacgaccta caccgaactg aga
//
