LOCUS       sequence-330359-        9848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP fused to progerin (d50) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Feric M, Demarest TG, Tian J, Croteau DL, Bohr VA, Misteli T
  TITLE     Self-assembly of multi-component mitochondrial nucleoids via phase 
            separation.
  JOURNAL   EMBO J. 2021 Mar 15;40(6):e107165. doi: 10.15252/embj.2020107165. 
            Epub 2021 Feb 23.
  PUBMED    33619770
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-03-15"; volume: "40(6):e107165. doi"; pages: " 
            10.15252/embj.2020107165. Epub 2021 Feb 23"
COMMENT     Sequence Label: pCDH_blast_MCS_Nard_GFP_D50
FEATURES             Location/Qualifiers
     source          1..9848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2088..2097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2094..2096,2097..2099,2100..2810)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2094..2096,2097..2099,2100..2810)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2139..2160)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2400..2419)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2747..2768
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4749..4960
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4973..5241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5273..5671
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5695..6283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5748..5768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6166..6177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6357..6590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6662..6783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6699..6718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6753..6772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6823..6958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6991..7007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6991..7007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7004..7026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7015..7031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7039..7045,7046..7063,7064..7069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7084..7105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7222..7239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7393..7981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8152..8943,8944..9012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8775..8794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9013..9117)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9185..9203
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9241..9263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9363..9382
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9576..9598
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9590..9607
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9591..9607
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9622..9848
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagagctagc gaattgggat ccatttaaat accggtcgcc accatggtga
     2101 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2161 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2221 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2281 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2341 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2401 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2461 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2521 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2581 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2641 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2701 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2761 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     2821 gatctcgaga tttcgaaaaa ggcgccgggt cgaccgagac cccgtcccag cggcgcgcca
     2881 cccgcagcgg ggcgcaggcc agctccactc cgctgtcgcc cacccgcatc acccggctgc
     2941 aggagaagga ggacctgcag gagctcaatg atcgcttggc ggtctacatc gaccgtgtgc
     3001 gctcgctgga aacggagaac gcagggctgc gccttcgcat caccgagtct gaagaggtgg
     3061 tcagccgcga ggtgtccggc atcaaggccg cctacgaggc cgagctcggg gatgcccgca
     3121 agacccttga ctcagtagcc aaggagcgcg cccgcctgca gctggagctg agcaaagtgc
     3181 gtgaggagtt taaggagctg aaagcgcgca ataccaagaa ggagggtgac ctgatagctg
     3241 ctcaggctcg gctgaaggac ctggaggctc tgctgaactc caaggaggcc gcactgagca
     3301 ctgctctcag tgagaagcgc acgctggagg gcgagctgca tgatctgcgg ggccaggtgg
     3361 ccaagcttga ggcagcccta ggtgaggcca agaagcaact tcaggatgag atgctgcggc
     3421 gggtggatgc tgagaacagg ctgcagacca tgaaggagga actggacttc cagaagaaca
     3481 tctacagtga ggagctgcgt gagaccaagc gccgtcatga gacccgactg gtggagattg
     3541 acaatgggaa gcagcgtgag tttgagagcc ggctggcgga tgcgctgcag gaactgcggg
     3601 cccagcatga ggaccaggtg gagcagtata agaaggagct ggagaagact tattctgcca
     3661 agctggacaa tgccaggcag tctgctgaga ggaacagcaa cctggtgggg gctgcccacg
     3721 aggagctgca gcagtcgcgc atccgcatcg acagcctctc tgcccagctc agccagctcc
     3781 agaagcagct ggcagccaag gaggcgaagc ttcgagacct ggaggactca ctggcccgtg
     3841 agcgggacac cagccggcgg ctgctggcgg aaaaggagcg ggagatggcc gagatgcggg
     3901 caaggatgca gcagcagctg gacgagtacc aggagcttct ggacatcaag ctggccctgg
     3961 acatggagat ccacgcctac cgcaagctct tggagggcga ggaggagagg ctacgcctgt
     4021 cccccagccc tacctcgcag cgcagccgtg gccgtgcttc ctctcactca tcccagacac
     4081 agggtggggg cagcgtcacc aaaaagcgca aactggagtc cactgagagc cgcagcagct
     4141 tctcacagca cgcacgcact agcgggcgcg tggccgtgga ggaggtggat gaggagggca
     4201 agtttgtccg gctgcgcaac aagtccaatg aggaccagtc catgggcaat tggcagatca
     4261 agcgccagaa tggagatgat cccttgctga cttaccggtt cccaccaaag ttcaccctga
     4321 aggctgggca ggtggtgacg atctgggctg caggagctgg ggccacccac agccccccta
     4381 ccgacctggt gtggaaggca cagaacacct ggggctgcgg gaacagcctg cgtacggctc
     4441 tcatcaactc cactggggaa gaagtggcca tgcgcaagct ggtgcgctca gtgactgtgg
     4501 ttgaggacga cgaggatgag gatggagatg acctgctcca tcaccaccac ggctcccact
     4561 gcagcagctc gggggacccc gctgagtaca acctgcgctc gcgcaccgtg ctgtgcggga
     4621 cctgcgggca gcctgccgac aaggcatctg ccagcggctc aggagcccag agcccccaga
     4681 actgcagcat catgtaagaa ttcacgcgta acgaacaagg atctgcgatc gctccggtgc
     4741 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     4801 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     4861 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     4921 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     4981 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     5041 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     5101 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     5161 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     5221 tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgcccatgg gcatggccaa
     5281 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     5341 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     5401 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     5461 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     5521 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     5581 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     5641 gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcgcgt cgacaatcaa
     5701 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5761 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5821 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5881 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5941 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6001 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6061 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6121 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6181 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6241 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     6301 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6361 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     6421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6601 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6661 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6781 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6841 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6901 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6961 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     7021 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7081 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8161 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8221 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8281 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8341 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8401 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8461 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8521 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8581 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8641 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8701 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8761 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9001 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9061 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9121 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9181 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9241 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9301 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9361 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     9421 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     9481 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     9541 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     9601 ggccagtgcc aagctgacgc gtgtagtctt atgcaatact cttgtagtct tgcaacatgg
     9661 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     9721 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     9781 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     9841 acataaac
//
