LOCUS       sequence-330360-       10009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP fused to lamin A (LA) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Feric M, Demarest TG, Tian J, Croteau DL, Bohr VA, Misteli T
  TITLE     Self-assembly of multi-component mitochondrial nucleoids via phase 
            separation.
  JOURNAL   EMBO J. 2021 Mar 15;40(6):e107165. doi: 10.15252/embj.2020107165. 
            Epub 2021 Feb 23.
  PUBMED    33619770
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-03-15"; volume: "40(6):e107165. doi"; pages: " 
            10.15252/embj.2020107165. Epub 2021 Feb 23"
COMMENT     Sequence Label: pCDH_blast_MCS_Nard_GFP_LAMIN
FEATURES             Location/Qualifiers
     source          1..10009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2008..2211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2208..2232
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2315..2324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2321..2323,2324..2326,2327..3037)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2321..2323,2324..2326,2327..3037)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2366..2387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2627..2646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2974..2995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5137..5348
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5361..5629
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5661..6059
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6083..6671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6136..6156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6554..6565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6745..6978
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7050..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7141..7160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7211..7346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7379..7395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7379..7395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7392..7414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7403..7419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7427..7433,7434..7451,7452..7457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7610..7627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7781..8369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8540..9331,9332..9400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9163..9182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9401..9505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9573..9591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9629..9651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9751..9770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9964..9986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9978..9995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9979..9995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccactgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgatactagt attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1981 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2041 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2101 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2161 acgcaaatgg gcggtaggcg tgtacggtgg gaggtttata taagcagagc tcgtttagtg
     2221 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagattctag
     2281 agctagcgaa ttgggatcca tttaaatacc ggtcgccacc atggtgagca agggcgagga
     2341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2401 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2581 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2701 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2761 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2821 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2881 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2941 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3001 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagattt
     3061 cgaaaaaggc gccgggtcga ccgagacccc gtcccagcgg cgcgccaccc gcagcggggc
     3121 gcaggccagc tccactccgc tgtcgcccac ccgcatcacc cggctgcagg agaaggagga
     3181 cctgcaggag ctcaatgatc gcttggcggt ctacatcgac cgtgtgcgct cgctggaaac
     3241 ggagaacgca gggctgcgcc ttcgcatcac cgagtctgaa gaggtggtca gccgcgaggt
     3301 gtccggcatc aaggccgcct acgaggccga gctcggggat gcccgcaaga cccttgactc
     3361 agtagccaag gagcgcgccc gcctgcagct ggagctgagc aaagtgcgtg aggagtttaa
     3421 ggagctgaaa gcgcgcaata ccaagaagga gggtgacctg atagctgctc aggctcggct
     3481 gaaggacctg gaggctctgc tgaactccaa ggaggccgca ctgagcactg ctctcagtga
     3541 gaagcgcacg ctggagggcg agctgcatga tctgcggggc caggtggcca agcttgaggc
     3601 agccctaggt gaggccaaga agcaacttca ggatgagatg ctgcggcggg tggatgctga
     3661 gaacaggctg cagaccatga aggaggaact ggacttccag aagaacatct acagtgagga
     3721 gctgcgtgag accaagcgcc gtcatgagac ccgactggtg gagattgaca atgggaagca
     3781 gcgtgagttt gagagccggc tggcggatgc gctgcaggaa ctgcgggccc agcatgagga
     3841 ccaggtggag cagtataaga aggagctgga gaagacttat tctgccaagc tggacaatgc
     3901 caggcagtct gctgagagga acagcaacct ggtgggggct gcccacgagg agctgcagca
     3961 gtcgcgcatc cgcatcgaca gcctctctgc ccagctcagc cagctccaga agcagctggc
     4021 agccaaggag gcgaagcttc gagacctgga ggactcactg gcccgtgagc gggacaccag
     4081 ccggcggctg ctggcggaaa aggagcggga gatggccgag atgcgggcaa ggatgcagca
     4141 gcagctggac gagtaccagg agcttctgga catcaagctg gccctggaca tggagatcca
     4201 cgcctaccgc aagctcttgg agggcgagga ggagaggcta cgcctgtccc ccagccctac
     4261 ctcgcagcgc agccgtggcc gtgcttcctc tcactcatcc cagacacagg gtgggggcag
     4321 cgtcaccaaa aagcgcaaac tggagtccac tgagagccgc agcagcttct cacagcacgc
     4381 acgcactagc gggcgcgtgg ccgtggagga ggtggatgag gagggcaagt ttgtccggct
     4441 gcgcaacaag tccaatgagg accagtccat gggcaattgg cagatcaagc gccagaatgg
     4501 agatgatccc ttgctgactt accggttccc accaaagttc accctgaagg ctgggcaggt
     4561 ggtgacgatc tgggctgcag gagctggggc cacccacagc ccccctaccg acctggtgtg
     4621 gaaggcacag aacacctggg gctgcgggaa cagcctgcgt acggctctca tcaactccac
     4681 tggggaagaa gtggccatgc gcaagctggt gcgctcagtg actgtggttg aggacgacga
     4741 ggatgaggat ggagatgacc tgctccatca ccaccacggc tcccactgca gcagctcggg
     4801 ggaccccgct gagtacaacc tgcgctcgcg caccgtgctg tgcgggacct gcgggcagcc
     4861 tgccgacaag gcatctgcca gcggctcagg agcccaggtg ggcggaccca tctcctctgg
     4921 ctcttctgcc tccagtgtca cggtcactcg cagctaccgc agtgtggggg gcagtggggg
     4981 tggcagcttc ggggacaatc tggtcacccg ctcctacctc ctgggcaact ccagcccccg
     5041 aacccagagc ccccagaact gcagcatcat gtaatctgtc tagaagaatt cacgcgtaac
     5101 gaacaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     5161 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     5221 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     5281 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     5341 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     5401 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     5461 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     5521 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     5581 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     5641 cggcgcctac gcccatgggc atggccaagc ctttgtctca agaagaatcc accctcattg
     5701 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     5761 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     5821 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     5881 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     5941 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     6001 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     6061 cacaattcga gctcgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6661 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6721 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc
     6781 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6841 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6901 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6961 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7021 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7321 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt
     7381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9541 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9601 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9661 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9721 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9781 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9841 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9901 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9961 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgacgcg
//
